##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631343.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	271163
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80682836522682	34.0	31.0	34.0	31.0	34.0
2	32.97235242271254	34.0	33.0	34.0	31.0	34.0
3	33.08667480445341	34.0	33.0	34.0	31.0	34.0
4	36.4228121093217	37.0	37.0	37.0	35.0	37.0
5	36.385251675191675	37.0	37.0	37.0	35.0	37.0
6	36.42714160855279	37.0	37.0	37.0	35.0	37.0
7	36.43072616839318	37.0	37.0	37.0	35.0	37.0
8	36.43001810719014	37.0	37.0	37.0	35.0	37.0
9	38.28094172140004	39.0	39.0	39.0	37.0	39.0
10	38.24078137504011	39.0	39.0	39.0	37.0	39.0
11	38.295645054819424	39.0	39.0	39.0	37.0	39.0
12	38.08173681512596	39.0	39.0	39.0	37.0	39.0
13	38.21159966514605	39.0	39.0	39.0	37.0	39.0
14	39.72983777285249	41.0	40.0	41.0	38.0	41.0
15	39.71722174485457	41.0	40.0	41.0	38.0	41.0
16	39.67787640644188	41.0	40.0	41.0	37.0	41.0
17	39.68354458388497	41.0	40.0	41.0	37.0	41.0
18	39.662575646382436	41.0	40.0	41.0	37.0	41.0
19	39.666658061756216	41.0	40.0	41.0	37.0	41.0
20	39.623930993535254	41.0	40.0	41.0	37.0	41.0
21	39.60102595118065	41.0	40.0	41.0	37.0	41.0
22	39.54139023391834	41.0	40.0	41.0	37.0	41.0
23	39.48300468721765	41.0	39.0	41.0	37.0	41.0
24	39.431260902114225	41.0	39.0	41.0	36.0	41.0
25	39.438964755516054	41.0	39.0	41.0	36.0	41.0
26	39.347093814421584	41.0	39.0	41.0	36.0	41.0
27	39.280974911768936	41.0	39.0	41.0	36.0	41.0
28	39.208243750069144	41.0	39.0	41.0	35.0	41.0
29	39.14357047237271	41.0	39.0	41.0	35.0	41.0
30	39.08664161408452	41.0	39.0	41.0	35.0	41.0
31	39.02744474725534	40.0	39.0	41.0	35.0	41.0
32	38.951342919203576	40.0	39.0	41.0	35.0	41.0
33	38.852037335477185	40.0	38.0	41.0	35.0	41.0
34	38.804925450743646	40.0	38.0	41.0	35.0	41.0
35	38.70519576785919	40.0	38.0	41.0	35.0	41.0
36	38.64245121937728	40.0	38.0	41.0	35.0	41.0
37	38.57244535574544	40.0	38.0	41.0	35.0	41.0
38	38.47680546387228	40.0	38.0	41.0	35.0	41.0
39	38.38567208653098	40.0	38.0	41.0	35.0	41.0
40	38.284736486910084	40.0	38.0	41.0	34.0	41.0
41	38.18169883059267	40.0	37.0	41.0	34.0	41.0
42	38.05530621803122	40.0	37.0	41.0	34.0	41.0
43	37.15090185607919	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	1.0
15	1.0
16	3.0
17	8.0
18	0.0
19	9.0
20	16.0
21	38.0
22	79.0
23	129.0
24	208.0
25	316.0
26	441.0
27	640.0
28	996.0
29	1384.0
30	1855.0
31	2383.0
32	3088.0
33	4030.0
34	5946.0
35	8478.0
36	13605.0
37	25608.0
38	54956.0
39	146942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86019847840598	18.34984861503966	12.659544259356917	26.130408647197438
2	19.55170875082515	20.141022189605515	33.365540283888286	26.941728775681046
3	20.340164402960582	21.632007316632432	28.91839963416838	29.109428646238612
4	15.218152919092946	15.686137120477351	32.974262712833244	36.121447247596464
5	16.515158779036962	34.43168868909106	31.835833059820107	17.217319472051866
6	34.74994744858259	33.28330192541018	15.790871173427053	16.175879452580183
7	29.970165546184397	29.646006276667542	20.44489845591028	19.93892972123778
8	28.177516844112212	31.38223135162246	19.673037988221107	20.767213816044226
9	27.321205326685426	14.358522364776904	19.635791018686177	38.68448128985149
10	19.242300756371627	25.191121207539375	30.49494215656266	25.071635879526337
11	37.064422506020364	21.11239365252634	20.315087235352905	21.50809660610039
12	23.05144875960216	25.16346256679562	27.086291271301764	24.698797402300464
13	31.097900524776612	18.415491789071517	24.077768722133918	26.40883896401795
14	23.75582214387656	20.232848876874794	23.862768888085764	32.14856009116288
15	26.879404638538446	26.24251833767881	21.261381530666057	25.616695493116687
16	26.05222688936175	24.970220863465887	23.33946740521384	25.638084841958523
17	24.930761202671455	25.579448523581753	24.142674332412607	25.34711594133418
18	25.132116107286024	23.58175709812917	25.78227855570266	25.50384823888215
19	25.49020330944856	24.83008375036417	25.181532878748207	24.498180061439058
20	25.96888218525389	23.882314327544687	25.01816250742174	25.130640979779688
21	27.10878696577335	24.070024302725667	24.478634621980138	24.34255410952084
22	26.60503092236035	23.96381512226963	24.20610481518496	25.225049140185057
23	25.60858229183185	23.766516818297482	24.80316267337358	25.821738216497085
24	25.11588970471635	24.65712505024653	24.722030660525217	25.504954584511896
25	25.389525857141276	23.68981018796812	24.925598256399287	25.995065698491317
26	25.177845059982374	24.61250244317993	25.029963527472404	25.179688969365287
27	25.719585636683473	23.784218348373486	24.637210828911023	25.85898518603202
28	24.720555533018885	24.37869473342602	25.547364500319	25.353385233236096
29	24.598857513746346	24.37758838779627	25.76236433436715	25.261189764090236
30	24.175127137551954	24.899414743161863	25.735074475499975	25.190383643786205
31	25.06942318826684	24.340341418261342	24.829714968487586	25.760520424984236
32	24.217168271482468	24.0224514406464	25.466601269347215	26.293779018523917
33	24.167013936267114	23.605727920107096	26.18351323742546	26.043744906200327
34	25.236481378359144	23.290050633751655	25.872998897342185	25.600469090547012
35	24.794311908335576	23.249853409204057	26.389293524559026	25.566541157901334
36	23.608678175119763	23.493618229625724	26.57220933534442	26.325494259910094
37	24.03093342380782	22.324948462732745	27.299815977843583	26.344302135615848
38	23.235470916017302	21.739322842718217	28.5835456902306	26.44166055103388
39	23.33430445894167	21.561938760081574	28.846487168234603	26.25726961274215
40	22.754579348952475	22.07970851480475	29.366838396093865	25.798873740148913
41	21.82377389245583	22.06606358537116	30.05904197844101	26.051120543731997
42	21.89863661340227	22.492006652825054	29.95357036173814	25.655786372034534
43	20.947916935570117	21.99894528383297	30.216143057865562	26.836994722731344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	76.0
1	67.0
2	58.0
3	116.5
4	175.0
5	175.0
6	217.0
7	259.0
8	267.0
9	275.0
10	393.5
11	512.0
12	512.0
13	853.0
14	1194.0
15	1684.5
16	2175.0
17	1991.5
18	1808.0
19	1808.0
20	2045.0
21	2282.0
22	1984.5
23	1687.0
24	1902.5
25	2118.0
26	2118.0
27	2440.0
28	2762.0
29	3337.5
30	3913.0
31	4607.5
32	5302.0
33	5302.0
34	6275.0
35	7248.0
36	8577.0
37	9906.0
38	11210.0
39	12514.0
40	12514.0
41	13408.0
42	14302.0
43	15174.0
44	16046.0
45	16899.5
46	17753.0
47	17753.0
48	18587.0
49	19421.0
50	19879.5
51	20338.0
52	20227.5
53	20117.0
54	20117.0
55	19722.0
56	19327.0
57	18536.0
58	17745.0
59	17208.5
60	16672.0
61	16672.0
62	15442.5
63	14213.0
64	12978.0
65	11743.0
66	10397.0
67	9051.0
68	9051.0
69	7938.5
70	6826.0
71	5894.0
72	4962.0
73	3973.5
74	2985.0
75	2985.0
76	2400.0
77	1815.0
78	1504.5
79	1194.0
80	1051.5
81	909.0
82	909.0
83	743.0
84	577.0
85	499.0
86	421.0
87	360.0
88	299.0
89	299.0
90	220.5
91	142.0
92	85.5
93	29.0
94	18.0
95	7.0
96	7.0
97	6.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	271163.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.54684494781179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.83898315150606	67.54467454008515
2	4.5212349172880115	6.650451268688282
3	1.25988998364037	2.7798279983429834
4	0.6257924422741237	1.8410023888578975
5	0.34302901936859975	1.2614351050051167
6	0.24394595259341925	1.0764873090620672
7	0.16523098558120367	0.8506552373980439
8	0.13705318614378525	0.8063863544736449
9	0.09027681506764837	0.5975617428146353
>10	0.678526774705735	9.722462080936191
>50	0.07290749693984071	3.699860297347594
>100	0.021620843920090685	2.206603337758429
>500	0.0010056206474460783	0.5805877727971733
>1k	5.028103237230392E-4	0.38200456643278985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1033	0.38095167851071127	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	851	0.31383337697252206	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	719	0.2651541692635057	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	330	0.12169801927254087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	7.375637531669144E-4	0.0
13	0.0	0.0	0.0	7.375637531669144E-4	0.0
14	0.0	0.0	0.0	7.375637531669144E-4	0.0
15	0.0	0.0	0.0	0.0014751275063338287	3.687818765834572E-4
16	0.0	0.0	0.0	0.001843909382917286	3.687818765834572E-4
17	0.0	0.0	0.0	0.0029502550126676574	3.687818765834572E-4
18	0.0	0.0	0.0	0.0029502550126676574	3.687818765834572E-4
19	0.0	0.0	0.0	0.0033190368892511146	3.687818765834572E-4
20	0.0	0.0	0.0	0.005900510025335315	3.687818765834572E-4
21	0.0	0.0	0.0	0.007006855655085686	3.687818765834572E-4
22	0.0	0.0	0.0	0.009588328791169887	3.687818765834572E-4
23	0.0	0.0	0.0	0.016226402569672116	3.687818765834572E-4
24	0.0	0.0	0.0	0.020283003212090145	3.687818765834572E-4
25	0.0	0.0	0.0	0.025445949484258545	3.687818765834572E-4
26	0.0	0.0	0.0	0.03909087891784646	3.687818765834572E-4
27	0.0	0.0	0.0	0.10805308983895295	3.687818765834572E-4
28	0.0	0.0	0.0	0.3271095245295265	3.687818765834572E-4
29	0.0	0.0	0.0	0.6188159889070411	3.687818765834572E-4
30	0.0	0.0	0.0	0.9931295936392502	3.687818765834572E-4
31	0.0	0.0	0.0	1.8339522722495325	3.687818765834572E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGGA	55	5.129178E-4	20.181818	7
GGTATCA	435	0.0	19.988506	1
CTATAAT	50	0.0070213648	18.5	1
CCGTCGT	50	0.0070213648	18.5	9
GAACACT	70	1.2148431E-4	18.5	6
TACCCTA	130	1.3793397E-8	17.076923	5
TCGGTGA	65	0.001575729	17.076923	13
CTATTCC	100	5.844542E-6	16.650002	4
ACAGTGC	80	3.3706563E-4	16.1875	8
ATGGTCG	80	3.3706563E-4	16.1875	5
GAGCGAA	70	0.0025856502	15.857142	16
CGATGCG	70	0.0025856502	15.857142	33
ATAACGC	70	0.0025856502	15.857142	3
CAGTCGG	70	0.0025856502	15.857142	10
ACCCGTT	130	2.5675035E-7	15.653846	30
CGCGGGT	145	5.2930773E-8	15.310346	22
CCCGTTG	135	3.9393854E-7	15.074075	31
GCGGGTA	135	3.9393854E-7	15.074075	23
CCGGCAG	150	8.021743E-8	14.8	14
CGGTGAT	75	0.0040936605	14.8	14
>>END_MODULE