FastQCFastQC Report
Fri 10 Feb 2017
ERR1631342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634840
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT207503.2685401045932836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143682.263247432423918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139392.1956713502614833No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.7171885829500346No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA43150.6796988217503623No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27060.42624913363997224No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA24610.3876567324050154No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT23900.37647281204712996No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT21980.3462289710793271No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT21020.3311070505954256No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT19410.30574632978388255No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA19390.3054312897738013No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18560.2923571293554281No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC18400.2898368092747779No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA18090.28495368911851804No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC17630.2777077688866486No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.26384600844307227No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA15910.2506143280196585No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA15120.23817024762144792No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA14610.2301367273643753No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14050.22131560708209944No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA13810.21753512696112406No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.1909142461092559No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT12040.18965408606893075No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT11530.18162056581185812No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA11400.17957280574632978No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.17547728561527312No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT10690.16838888538844432No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT10670.16807384537836303No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT10440.16445088526242832No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA9420.14838384474828303No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA9230.14539096465251086No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG9180.14460336462730766No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.14444584462226703No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA8670.13656984437023503No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.13089912418877198No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8200.12916640413332495No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.12853632411316238No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC8100.12759120408291852No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC7640.12034528385104909No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC7580.11940016382080525No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7460.11750992376031756No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT7330.11546216369478925No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT7240.11404448364942348No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA7120.11215424358893579No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.10837376346796042No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA6760.10648352340747276No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT6540.10301808329657867No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT6510.10254552328145675No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT6360.10018272320584715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAG200.001841315437.02
GTTATAC507.3214323E-933.3000033
TTTACGC250.005494511729.63
TAACCCT250.005494511729.635
AACGAGG250.005494511729.629
GTCCCTC250.005494511729.61
TAGGACG250.005494511729.64
ATATGCT1350.028.77777935
CAGTATC652.9849616E-928.4615381
TATGCTG1450.028.06896636
CTTATTC604.316462E-827.74999828
TATACCA604.316462E-827.7499985
CTAGAGA358.865002E-426.4285724
AACAGAG358.865002E-426.4285722
TACCCTC509.082716E-625.9000025
GGTACAT509.082716E-625.9000021
GCGTTAT601.3358313E-624.6666661
ATTGACT5100.024.3039231
ATGCCCC3750.024.17333437
TATGGCA701.920107E-723.7857154
Produced by FastQC (version 0.11.5)