##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631342.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634840
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78922405645517	34.0	31.0	34.0	31.0	34.0
2	32.89910056077122	34.0	33.0	34.0	31.0	34.0
3	32.91875433180014	34.0	33.0	34.0	31.0	34.0
4	36.33844275723017	37.0	37.0	37.0	35.0	37.0
5	36.35709155062693	37.0	37.0	37.0	35.0	37.0
6	36.44200901014429	37.0	37.0	37.0	35.0	37.0
7	36.4403062188898	37.0	37.0	37.0	35.0	37.0
8	36.46442410686157	37.0	37.0	37.0	35.0	37.0
9	38.27957280574633	39.0	39.0	39.0	37.0	39.0
10	38.27041144225316	39.0	39.0	39.0	37.0	39.0
11	38.31736658055573	39.0	39.0	39.0	37.0	39.0
12	38.11198097158339	39.0	39.0	39.0	37.0	39.0
13	38.23181904101821	39.0	39.0	39.0	37.0	39.0
14	39.58616501795728	41.0	39.0	41.0	37.0	41.0
15	39.54663852309243	41.0	39.0	41.0	37.0	41.0
16	39.4796531409489	41.0	39.0	41.0	37.0	41.0
17	39.486371369163884	41.0	39.0	41.0	37.0	41.0
18	39.682384537836306	41.0	39.0	41.0	38.0	41.0
19	39.716131623716215	41.0	40.0	41.0	38.0	41.0
20	39.75554785457753	41.0	40.0	41.0	38.0	41.0
21	39.739928170877704	41.0	40.0	41.0	37.0	41.0
22	39.660960241950725	41.0	40.0	41.0	37.0	41.0
23	39.57363587675635	41.0	40.0	41.0	36.0	41.0
24	39.531871022619875	41.0	39.0	41.0	36.0	41.0
25	39.491349001323165	41.0	39.0	41.0	36.0	41.0
26	39.33367935227774	41.0	39.0	41.0	35.0	41.0
27	39.17329563354546	41.0	39.0	41.0	35.0	41.0
28	38.99905172956966	41.0	38.0	41.0	35.0	41.0
29	38.74430880221788	40.0	38.0	41.0	35.0	41.0
30	38.46362863083612	40.0	37.0	41.0	35.0	41.0
31	38.134364564299666	40.0	36.0	41.0	35.0	41.0
32	37.86482105727428	40.0	35.0	41.0	35.0	41.0
33	37.612831579610614	40.0	35.0	41.0	35.0	41.0
34	37.4056029865793	40.0	35.0	41.0	34.0	41.0
35	37.16208493478672	39.0	35.0	41.0	33.0	41.0
36	36.939906118077	39.0	35.0	41.0	33.0	41.0
37	36.682531031440995	39.0	35.0	41.0	33.0	41.0
38	36.40855963707391	39.0	35.0	41.0	33.0	41.0
39	36.05278652888917	39.0	35.0	41.0	31.0	41.0
40	35.731226765799256	39.0	35.0	41.0	30.0	41.0
41	35.31562755970008	39.0	35.0	41.0	25.0	41.0
42	34.88744250519816	38.0	35.0	41.0	21.0	41.0
43	33.81234799319513	37.0	34.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	6.0
12	7.0
13	8.0
14	14.0
15	4.0
16	7.0
17	22.0
18	15.0
19	35.0
20	59.0
21	125.0
22	227.0
23	385.0
24	756.0
25	1145.0
26	1822.0
27	2860.0
28	4430.0
29	6398.0
30	8020.0
31	9479.0
32	11152.0
33	14388.0
34	19402.0
35	27008.0
36	43536.0
37	112329.0
38	125598.0
39	245596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.335297082729504	13.330760506584335	16.97293806313402	23.36100434755214
2	19.256505576208177	22.22512759120408	34.23618549555793	24.282181337029805
3	19.357318379434187	24.495778463864912	30.351269611240628	25.795633545460273
4	12.840715770902905	19.55280070568962	30.82445970638271	36.782023817024765
5	13.019500976624032	40.22289080713251	29.37291285993321	17.38469535631025
6	26.069875874236025	32.810471929935105	21.779188456934033	19.34046373889484
7	29.324239178375656	32.25048831201563	17.553714321718857	20.871558187889864
8	35.71561338289963	28.74267531976561	16.475804927225756	19.065906370109005
9	32.04917774557369	12.872692331926155	16.41972780543129	38.65840211706887
10	21.09019595488627	28.237351143595234	21.20109003843488	29.471362863083613
11	33.36147690756726	27.445340558250898	14.909425997101632	24.283756537080208
12	25.36402873164892	31.592842290970953	23.308077625858484	19.735051351521644
13	35.97315859114107	19.139940772478102	24.582729506647343	20.304171129733476
14	24.162938693214038	21.820143658244596	26.337344842795034	27.67957280574633
15	31.029865792955707	25.816268666120596	21.42004284544137	21.733822695482328
16	21.803604057715333	27.22418247117384	26.415002205280068	24.557211265830762
17	17.43147879780732	32.05894398588621	27.487083359586666	23.0224938567198
18	21.471709407094703	23.28539474513263	31.462573246802343	23.780322600970326
19	20.990958351710667	30.00661584021171	32.38170247621448	16.620723331863147
20	23.92476844559259	23.32934282653897	34.32345157835045	18.42243714951799
21	27.619557683825846	21.473284607145107	31.514712368470793	19.39244534055825
22	25.515405456492974	22.893642492596562	27.828114170499653	23.76283788041081
23	21.41972780543129	25.981034591393108	28.517106672547417	24.08213093062819
24	20.399785772793145	25.53241761703736	33.61130363556172	20.456492974607777
25	19.573593346354986	25.291884569340304	30.404511372944366	24.730010711360343
26	24.09914309117258	23.780795160985445	29.80278495368912	22.317276794152857
27	21.60418373133388	24.507434944237918	28.975332367210637	24.91304895721757
28	19.509167664293365	25.106641043412516	32.715802406905674	22.668388885388442
29	19.58194190662214	26.029393232940585	34.54555478545775	19.843110074979524
30	19.02526620880852	26.57630269044169	35.90763026904417	18.490800831705627
31	19.241383655724277	28.567985634175542	31.380505324176173	20.810125385924014
32	18.046594417491022	27.838510490832334	35.43743305399786	18.677462037678787
33	20.191544326129417	25.423098733539156	35.16523848528763	19.220118455043792
34	21.018209312582698	26.795255497448178	29.93415663789301	22.252378552076113
35	18.847268603112596	28.10440425934094	31.14406779661017	21.9042593409363
36	16.250708840022682	27.144319828618237	33.06817465818159	23.53679667317749
37	17.245447671854325	27.37508663600277	33.42007434944238	21.959391342700524
38	17.921838573498835	23.480246991367903	35.4712998550816	23.126614580051665
39	18.406527629008885	23.468748030999937	36.49927540797681	21.625448932014365
40	17.293648793396763	23.464179950853758	33.8883813244282	25.35378993132128
41	13.935794845945434	25.26605128851364	36.96143910276605	23.83671476277487
42	14.28911221725159	24.309904857916955	35.60393169932581	25.79705122550564
43	13.074632978388257	27.27317119274148	37.096433747085875	22.555762081784387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	92.5
2	157.0
3	787.5
4	1418.0
5	1418.0
6	2079.5
7	2741.0
8	2918.5
9	3096.0
10	4979.0
11	6862.0
12	6862.0
13	11489.0
14	16116.0
15	27359.5
16	38603.0
17	33682.0
18	28761.0
19	28761.0
20	30520.0
21	32279.0
22	22449.5
23	12620.0
24	10454.0
25	8288.0
26	8288.0
27	7196.0
28	6104.0
29	5983.0
30	5862.0
31	5806.0
32	5750.0
33	5750.0
34	5747.0
35	5744.0
36	5873.5
37	6003.0
38	7365.0
39	8727.0
40	8727.0
41	12016.5
42	15306.0
43	20741.5
44	26177.0
45	34639.5
46	43102.0
47	43102.0
48	58574.5
49	74047.0
50	69975.5
51	65904.0
52	51836.0
53	37768.0
54	37768.0
55	31181.5
56	24595.0
57	23966.5
58	23338.0
59	22892.0
60	22446.0
61	22446.0
62	22391.5
63	22337.0
64	21944.0
65	21551.0
66	20590.0
67	19629.0
68	19629.0
69	18109.0
70	16589.0
71	14636.0
72	12683.0
73	10811.0
74	8939.0
75	8939.0
76	7266.0
77	5593.0
78	4386.5
79	3180.0
80	2368.5
81	1557.0
82	1557.0
83	1100.5
84	644.0
85	425.0
86	206.0
87	138.0
88	70.0
89	70.0
90	43.0
91	16.0
92	9.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	634840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.87019383566459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.82125603508602	40.69329099420346
2	7.383599800617581	6.921415077198407
3	2.0278219641133424	2.8513322556663128
4	0.9495007239272126	1.780131319102892
5	0.5348317523937446	1.2533833952081488
6	0.35807849905151834	1.0069925195357101
7	0.2544307643725625	0.8347653470728813
8	0.19446102724717795	0.7291540832446147
9	0.15011329559159797	0.633225533551975
>10	1.0771169707880395	10.094208857599037
>50	0.12523850883233548	4.01530112755134
>100	0.10262131154531201	9.961516213446666
>500	0.01080224332055916	3.5681720718813406
>1k	0.009114392801721792	7.895324341918711
>5k	0.0	0.0
>10k+	0.0010127103113024211	7.761786862818505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20750	3.2685401045932836	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14368	2.263247432423918	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13939	2.1956713502614833	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4553	0.7171885829500346	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	4315	0.6796988217503623	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2706	0.42624913363997224	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	2461	0.3876567324050154	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	2390	0.37647281204712996	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	2198	0.3462289710793271	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	2102	0.3311070505954256	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	1941	0.30574632978388255	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	1939	0.3054312897738013	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1856	0.2923571293554281	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	1840	0.2898368092747779	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	1809	0.28495368911851804	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	1763	0.2777077688866486	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1675	0.26384600844307227	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1591	0.2506143280196585	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	1512	0.23817024762144792	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1461	0.2301367273643753	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1405	0.22131560708209944	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	1381	0.21753512696112406	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.1909142461092559	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	1204	0.18965408606893075	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	1153	0.18162056581185812	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	1140	0.17957280574632978	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1114	0.17547728561527312	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	1069	0.16838888538844432	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	1067	0.16807384537836303	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	1044	0.16445088526242832	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	942	0.14838384474828303	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	923	0.14539096465251086	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	918	0.14460336462730766	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	917	0.14444584462226703	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	867	0.13656984437023503	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.13089912418877198	No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	820	0.12916640413332495	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	816	0.12853632411316238	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	810	0.12759120408291852	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	764	0.12034528385104909	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	758	0.11940016382080525	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	746	0.11750992376031756	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	733	0.11546216369478925	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	724	0.11404448364942348	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	712	0.11215424358893579	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	688	0.10837376346796042	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	676	0.10648352340747276	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	654	0.10301808329657867	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	651	0.10254552328145675	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	636	0.10018272320584715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.876000252032008E-4	0.0
2	0.0	0.0	0.0	7.876000252032008E-4	0.0
3	0.0	0.0	0.0	0.0017327200554470418	0.0
4	0.0	0.0	0.0	0.0017327200554470418	0.0
5	0.0	0.0	0.0	0.0017327200554470418	0.0
6	0.0	0.0	0.0	0.0017327200554470418	0.0
7	0.0	0.0	0.0	0.0017327200554470418	0.0
8	0.0	0.0	0.0	0.007718480246991368	0.0
9	0.0	0.0	0.0	0.01039632033268225	0.0
10	0.0	0.0	0.0	0.01795728057463298	0.0
11	0.0	0.0	0.0	0.022682880725852183	0.0
12	0.0	0.0	0.0	0.022840400730892822	0.0
13	0.0	0.0	0.0	0.022840400730892822	0.0
14	0.0	0.0	0.0	0.023943040766177304	0.0
15	0.0	0.0	0.0	0.024888160796421144	0.0
16	0.0	0.0	0.0	0.02835360090731523	0.0
17	0.0	0.0	0.0	0.03150400100812803	0.0
18	0.0	0.0	0.0	0.03244912103837187	0.0
19	0.0	0.0	0.0	0.03465440110894084	0.0
20	0.0	0.0	0.0	0.035757041144225316	0.0
21	0.0	0.0	0.0	0.0400100812803226	0.0
22	0.0	0.0	0.0	0.04631088148194821	0.0
23	0.0	0.0	0.0	0.062062881986012224	0.0
24	0.0	0.0	0.0	0.08836872282779913	0.0
25	0.0	0.0	0.0	0.09750488312015626	0.0
26	0.0	0.0	0.0	0.11577720370487052	0.0
27	0.0	0.0	0.0	0.1816205658118581	0.0
28	0.0	0.0	0.0	0.4345976939071262	0.0
29	0.0	0.0	0.0	0.8918782685401045	0.0
30	0.0	0.0	0.0	1.6022934912733917	0.0
31	0.0	0.0	0.0	3.0539978577279316	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAG	20	0.0018413154	37.0	2
GTTATAC	50	7.3214323E-9	33.300003	3
TTTACGC	25	0.0054945117	29.6	3
TAACCCT	25	0.0054945117	29.6	35
AACGAGG	25	0.0054945117	29.6	29
GTCCCTC	25	0.0054945117	29.6	1
TAGGACG	25	0.0054945117	29.6	4
ATATGCT	135	0.0	28.777779	35
CAGTATC	65	2.9849616E-9	28.461538	1
TATGCTG	145	0.0	28.068966	36
CTTATTC	60	4.316462E-8	27.749998	28
TATACCA	60	4.316462E-8	27.749998	5
CTAGAGA	35	8.865002E-4	26.428572	4
AACAGAG	35	8.865002E-4	26.428572	2
TACCCTC	50	9.082716E-6	25.900002	5
GGTACAT	50	9.082716E-6	25.900002	1
GCGTTAT	60	1.3358313E-6	24.666666	1
ATTGACT	510	0.0	24.30392	31
ATGCCCC	375	0.0	24.173334	37
TATGGCA	70	1.920107E-7	23.785715	4
>>END_MODULE