FastQCFastQC Report
Fri 10 Feb 2017
ERR1631341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939060
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40160.42766170425744887No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC36620.38996443251762397No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT34080.36291610759695864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33870.36067982876493515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28840.3071156262645624No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA24610.26207058121951743No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG23640.2517411028049326No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC21280.226609588311716No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG19990.21287244691500012No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC19990.21287244691500012No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT19150.20392733158690604No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG18240.19423678998147084No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT18150.19327838476774648No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG18030.19200051114944733No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT17310.18433326943965242No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16850.179434753902839No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT16590.1766660277298575No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT16440.17506868570698358No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC15570.16580410197431475No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG15560.16569761250612314No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC15470.16473920729239877No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG15040.16016016016016016No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT14730.1568589866462207No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14460.1539837710050476No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG14120.15036312908653335No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG14070.14983068174557537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.13950120333099056No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG13070.13918173492641578No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG12790.1362000298170511No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12630.13449619832598556No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC12600.13417672992141078No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC12600.13417672992141078No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG12150.12938470385278894No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA12110.12895874598002258No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA11920.12693544608438226No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC11840.1260835303388495No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC11500.12246288842033522No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA11490.12235639895214363No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT11390.12129150427022768No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC11370.12107852533384449No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT11320.1205460779928865No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA11130.11852277809724619No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG11110.118309799160863No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC11090.1180968202244798No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG10920.11628649926522266No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG10770.11468915724234872No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT10760.11458266777415714No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC10710.11405022043319915No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG10590.1127723468149No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC10520.11202692053755885No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG10480.11160096266479246No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT10430.11106851532383447No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT10420.11096202585564288No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA10390.11064255745106809No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG10170.10829978915085298No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA10150.1080868102144698No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT10120.10776734180989501No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC10010.10659595765978745No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG9820.10457265776414712No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC9810.10446616829595554No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC9640.1026558473366984No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC9600.10222988946393201No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT9520.10137797371839925No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG9500.10116499478201606No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG9470.10084552637744128No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG9450.1006325474410581No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC9400.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC250.005495837429.63
TCAAGCC1054.0017767E-1122.9047631
TGCATAA753.73926E-722.214
TGCATCA9100.020.73626314
TAAGGCT1251.8189894E-1120.7234
CAGGACA9950.020.6381914
TAGTCCT450.003825295320.55555511
ATTAACT555.142131E-420.18181824
GGGGTTA555.142131E-420.1818186
TGAATTA555.142131E-420.18181821
TCCAGGA10000.020.1649992
CTGCATC9300.020.09139813
GCATCAG9300.020.09139815
CAAGCCT1202.382876E-1020.04166632
CCCCTAT656.900409E-519.9230791
TATGGCT656.900409E-519.92307935
TTATGGC656.900409E-519.92307934
ACTAGAA656.900409E-519.92307928
CTGCATA656.900409E-519.92307913
GGTATCA18150.019.5702481
Produced by FastQC (version 0.11.5)