FastQCFastQC Report
Fri 10 Feb 2017
ERR1631340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255732
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32971.2892402984374267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28831.1273520717000611No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22890.8950776594247102No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.410195047940813No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4240.16579857037836487No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.15719581436816665No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.13803513052727073No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.11770134359407505No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2800.10948962194797679No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.10714341576337728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGA200.001839261337.01
CGAACGA358.850469E-426.4285716
ATAACGA358.850469E-426.4285712
CCGATAA400.001927105623.1259
TAACGAA400.001927105623.12513
CCGCAGA400.001927105623.12514
AACGAAC450.003817799720.55555514
CGATAAC450.003817799720.55555510
ACGAACG450.003817799720.55555515
GCGTTAT555.128077E-420.1818181
TCCGCTT759.219648E-619.73333424
GCAGTCG3150.018.793659
CAGTCGG3200.018.510
CGCTTAT801.6086746E-518.526
AGGTCAA500.007020298418.49999810
ATGGGGC500.007020298418.49999812
GATAACG500.007020298418.49999811
CATGCTT500.007020298418.49999810
TCCGATA500.007020298418.4999988
CCGATCG500.007020298418.49999814
Produced by FastQC (version 0.11.5)