##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631340.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255732
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72842272378897	34.0	31.0	34.0	31.0	34.0
2	32.866496957752645	34.0	31.0	34.0	31.0	34.0
3	32.95406910359282	34.0	33.0	34.0	31.0	34.0
4	36.32939561728685	37.0	37.0	37.0	35.0	37.0
5	36.30902272691724	37.0	37.0	37.0	35.0	37.0
6	36.36906996386843	37.0	37.0	37.0	35.0	37.0
7	36.38444934540848	37.0	37.0	37.0	35.0	37.0
8	36.3943894389439	37.0	37.0	37.0	35.0	37.0
9	38.20469084823174	39.0	39.0	39.0	37.0	39.0
10	38.1684341419924	39.0	38.0	39.0	37.0	39.0
11	38.2258223452677	39.0	39.0	39.0	37.0	39.0
12	38.029570018613235	39.0	38.0	39.0	37.0	39.0
13	38.15726620055371	39.0	39.0	39.0	37.0	39.0
14	39.605219526692004	41.0	39.0	41.0	37.0	41.0
15	39.579544210345205	41.0	39.0	41.0	37.0	41.0
16	39.518636697793	41.0	39.0	41.0	37.0	41.0
17	39.551323260288115	41.0	39.0	41.0	37.0	41.0
18	39.57801917632521	41.0	39.0	41.0	37.0	41.0
19	39.600312045422555	41.0	40.0	41.0	37.0	41.0
20	39.57104703361331	41.0	40.0	41.0	37.0	41.0
21	39.54372155225001	41.0	40.0	41.0	37.0	41.0
22	39.463418735238456	41.0	39.0	41.0	37.0	41.0
23	39.392277853377756	41.0	39.0	41.0	36.0	41.0
24	39.33808440085715	41.0	39.0	41.0	36.0	41.0
25	39.336676677146386	41.0	39.0	41.0	36.0	41.0
26	39.21051726025683	41.0	39.0	41.0	35.0	41.0
27	39.104668168238625	41.0	39.0	41.0	35.0	41.0
28	38.984816135642	40.0	39.0	41.0	35.0	41.0
29	38.86578527520999	40.0	38.0	41.0	35.0	41.0
30	38.76403422332755	40.0	38.0	41.0	35.0	41.0
31	38.622913831667525	40.0	38.0	41.0	35.0	41.0
32	38.48200850890776	40.0	38.0	41.0	35.0	41.0
33	38.317562917429186	40.0	37.0	41.0	35.0	41.0
34	38.22151705691896	40.0	37.0	41.0	35.0	41.0
35	38.067562917429186	40.0	37.0	41.0	34.0	41.0
36	37.94340168614018	40.0	37.0	41.0	34.0	41.0
37	37.798011199224185	40.0	37.0	41.0	34.0	41.0
38	37.63807814430732	40.0	36.0	41.0	33.0	41.0
39	37.47022273317379	40.0	36.0	41.0	33.0	41.0
40	37.265168222983434	40.0	35.0	41.0	33.0	41.0
41	37.06369558756823	40.0	35.0	41.0	33.0	41.0
42	36.84136908951559	40.0	35.0	41.0	32.0	41.0
43	35.90451331863044	39.0	35.0	41.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	1.0
15	3.0
16	1.0
17	1.0
18	10.0
19	18.0
20	39.0
21	56.0
22	118.0
23	163.0
24	271.0
25	404.0
26	659.0
27	912.0
28	1349.0
29	1925.0
30	2591.0
31	3075.0
32	3768.0
33	4866.0
34	6531.0
35	9295.0
36	14533.0
37	30967.0
38	54565.0
39	119609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.605680947241645	18.004786260616584	13.6838565373125	23.705676254829275
2	19.39452239062769	21.714529272832497	33.114745123801484	25.776203212738334
3	20.71778267874181	23.381508766990443	29.56102482286143	26.339683731406318
4	14.24929222780098	16.88799211674722	33.003691364397106	35.859024291054695
5	14.704847261977383	37.10681494689753	31.64914832715499	16.539189463970093
6	31.708194516134082	33.42561744326091	17.59420017831167	17.27198786229334
7	28.68862715655452	31.539658705207014	20.390486915990177	19.38122722224829
8	30.345830791609966	30.04551639998123	19.657688517666934	19.950964290741872
9	28.144307321727435	14.021710227894827	20.13123113259193	37.702751317785804
10	19.94666291273677	26.683402937450147	28.94553673376816	24.424397416044922
11	34.441133686828394	23.376816354621244	19.61975818434924	22.562291774201118
12	23.198504684591683	28.130621118983935	26.440179562979992	22.230694633444386
13	31.525581468099418	19.412509971376284	25.466112962007102	23.595795598517196
14	23.237217086637575	21.834576822611172	25.76095287253844	29.167253218212817
15	27.104937981949856	26.918414590274192	22.657313124677398	23.319334303098557
16	23.975881000422316	25.9326169583783	25.70894530211315	24.38255673908623
17	22.45944973644284	27.860416373390894	25.846198363912222	23.833935526254045
18	22.698762767271987	23.85114103827444	28.924029843742666	24.526066350710902
19	23.316597062549857	26.46442369355419	29.084353933023632	21.13462531087232
20	24.60857460153598	23.607135595076095	29.060500836813542	22.723788966574382
21	25.996746594090688	24.008336852642607	28.077831479830444	21.917085073436255
22	25.347629549684825	24.172180251200476	27.350507562604605	23.129682636510097
23	23.44915771197973	24.72627594513006	27.759529507453113	24.0650368354371
24	22.507546963227128	25.659674972236562	28.88023399496348	22.952544069572834
25	22.794957220840566	25.120047549778672	28.00627219120016	24.0787230381806
26	23.557474230835407	25.494267436222295	27.969905995338873	22.97835233760343
27	23.514069416420316	24.635164938294775	27.656687469694834	24.194078175590068
28	22.247509111100683	25.36366195861292	28.977210517260254	23.411618413026137
29	22.11573053039901	25.62369981073937	29.599346190543223	22.661223468318394
30	21.85178233463157	26.22902100636604	29.602865499820126	22.31633115918227
31	22.22522015234699	26.74127602333693	28.20882799180392	22.824675832512163
32	21.723132028842695	25.98423349443949	28.987768445090957	23.304866031626858
33	21.424772809034458	25.695650133733754	29.433547620164862	23.44602943706693
34	22.965057169224032	25.379303333176917	28.06727355199975	23.5883659455993
35	22.12667949259381	25.69995151173885	28.58617615316034	23.587192842506997
36	20.7572771495159	26.397947851657204	28.893138128978773	23.951636869848123
37	21.64531619038681	25.022679993117798	29.511754492984842	23.820249323510552
38	21.358296967137473	24.130339574241784	30.31767631739477	24.19368714122597
39	21.777485805452585	23.974316862965917	30.468615581937343	23.77958174964416
40	20.67046752068572	24.15771197972878	30.52062315236263	24.651197347222876
41	19.361284469679198	24.486962914300907	31.800478626061658	24.351273989958237
42	19.963477390393066	24.24256643673846	31.6127821312937	24.181174041574774
43	19.00544319834827	24.470930505372813	31.718361409600675	24.80526488667824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	127.5
2	117.0
3	288.0
4	459.0
5	459.0
6	600.0
7	741.0
8	736.0
9	731.0
10	1120.5
11	1510.0
12	1510.0
13	2499.0
14	3488.0
15	5181.0
16	6874.0
17	6046.5
18	5219.0
19	5219.0
20	5794.0
21	6369.0
22	4828.0
23	3287.0
24	3321.5
25	3356.0
26	3356.0
27	3606.5
28	3857.0
29	4255.5
30	4654.0
31	5234.0
32	5814.0
33	5814.0
34	6417.0
35	7020.0
36	7891.5
37	8763.0
38	9409.5
39	10056.0
40	10056.0
41	10758.5
42	11461.0
43	12263.5
44	13066.0
45	14276.5
46	15487.0
47	15487.0
48	17551.0
49	19615.0
50	19101.0
51	18587.0
52	17575.5
53	16564.0
54	16564.0
55	15587.0
56	14610.0
57	14228.0
58	13846.0
59	13612.5
60	13379.0
61	13379.0
62	12636.0
63	11893.0
64	11205.5
65	10518.0
66	9312.5
67	8107.0
68	8107.0
69	7169.5
70	6232.0
71	5244.5
72	4257.0
73	3187.5
74	2118.0
75	2118.0
76	1639.0
77	1160.0
78	974.5
79	789.0
80	690.5
81	592.0
82	592.0
83	478.5
84	365.0
85	325.0
86	285.0
87	248.5
88	212.0
89	212.0
90	158.5
91	105.0
92	60.5
93	16.0
94	14.0
95	12.0
96	12.0
97	7.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	255732.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.27775312499578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.77357414360408	57.4394781493248
2	4.4311593214531575	5.607876112008736
3	1.4638562536791504	2.7788860389237144
4	0.7315975616526059	1.8517539957240994
5	0.4570145739041539	1.4459427691016098
6	0.3398235022815623	1.2901960610066483
7	0.2344243112393336	1.038369058516981
8	0.17763970023008044	0.8992512877085829
9	0.12416670499307214	0.7071291094406219
>10	1.1097226536438802	13.947011129636252
>50	0.11171473991068426	4.925955063257752
>100	0.042823983632428966	4.330190586580561
>500	0.0	0.0
>1k	0.002482549775792984	3.7379606387696156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3297	1.2892402984374267	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2883	1.1273520717000611	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2289	0.8950776594247102	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.410195047940813	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	424	0.16579857037836487	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	402	0.15719581436816665	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	353	0.13803513052727073	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	301	0.11770134359407505	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	280	0.10948962194797679	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	274	0.10714341576337728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	3.910343640999171E-4
8	0.0	0.0	0.0	0.0	3.910343640999171E-4
9	0.0	0.0	0.0	0.0	3.910343640999171E-4
10	0.0	0.0	0.0	7.820687281998342E-4	3.910343640999171E-4
11	0.0	0.0	0.0	0.0015641374563996684	3.910343640999171E-4
12	0.0	0.0	0.0	0.0023462061845995028	3.910343640999171E-4
13	0.0	0.0	0.0	0.00273724054869942	3.910343640999171E-4
14	0.0	0.0	0.0	0.003910343640999171	3.910343640999171E-4
15	0.0	0.0	0.0	0.005083446733298923	3.910343640999171E-4
16	0.0	0.0	0.0	0.00821172164609826	3.910343640999171E-4
17	0.0	0.0	0.0	0.014468271471696933	3.910343640999171E-4
18	0.0	0.0	0.0	0.016814477656296435	3.910343640999171E-4
19	0.0	0.0	0.0	0.018378615112696103	3.910343640999171E-4
20	0.0	0.0	0.0	0.025026199302394695	3.910343640999171E-4
21	0.0	0.0	0.0	0.030109646035693615	3.910343640999171E-4
22	0.0	0.0	0.0	0.04066757386639138	3.910343640999171E-4
23	0.0	0.0	0.0	0.05591791406628815	3.910343640999171E-4
24	0.0	0.0	0.0	0.07664273536358375	3.910343640999171E-4
25	0.0	0.0	0.0	0.08602756010198176	3.910343640999171E-4
26	0.0	0.0	0.0	0.10675238139927737	3.910343640999171E-4
27	0.0	0.0	0.0	0.17283718893216335	3.910343640999171E-4
28	0.0	0.0	0.0	0.3558412713309246	3.910343640999171E-4
29	0.0	0.0	0.0	0.6186163640060689	3.910343640999171E-4
30	0.0	0.0	0.0	0.9556879858601974	3.910343640999171E-4
31	0.0	0.0	0.0	1.622401576650556	3.910343640999171E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTGA	20	0.0018392613	37.0	1
CGAACGA	35	8.850469E-4	26.42857	16
ATAACGA	35	8.850469E-4	26.42857	12
CCGATAA	40	0.0019271056	23.125	9
TAACGAA	40	0.0019271056	23.125	13
CCGCAGA	40	0.0019271056	23.125	14
AACGAAC	45	0.0038177997	20.555555	14
CGATAAC	45	0.0038177997	20.555555	10
ACGAACG	45	0.0038177997	20.555555	15
GCGTTAT	55	5.128077E-4	20.181818	1
TCCGCTT	75	9.219648E-6	19.733334	24
GCAGTCG	315	0.0	18.79365	9
CAGTCGG	320	0.0	18.5	10
CGCTTAT	80	1.6086746E-5	18.5	26
AGGTCAA	50	0.0070202984	18.499998	10
ATGGGGC	50	0.0070202984	18.499998	12
GATAACG	50	0.0070202984	18.499998	11
CATGCTT	50	0.0070202984	18.499998	10
TCCGATA	50	0.0070202984	18.499998	8
CCGATCG	50	0.0070202984	18.499998	14
>>END_MODULE