FastQCFastQC Report
Fri 10 Feb 2017
ERR1631339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274225
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.2131275489452451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40720.17905000604601568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34770.15288724730402664No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA34020.14958942057184316No Hit
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG28710.12624080730798404No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC28710.12624080730798404No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC25220.11089492024755686No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23110.1016170343743473No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC22960.10095746902791061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT4300.021.9418627
CTAGACA5050.021.2475244
ACATGTA3600.021.0694458
TCTAGAC4950.020.9292933
CGTAAGT450.003826943520.55555512
GTCTAGA4850.019.8350521
TAGAACT5700.019.7982464
GACATGT4000.019.4250017
ACACGCA1052.2622771E-819.38095337
AGTGCGC5850.019.290629
GGTATCA24150.018.844721
GTCTATA500.007036947618.51
AGTCGGT4500.018.08888811
CCTATCG4200.018.05952519
ATACACA4850.017.92783537
GTCAAAA5400.017.81481631
CTAGAAC5300.017.8018883
ACGGAGT3250.017.64615426
GTGCGCT6100.017.59016230
GTTCTAG6000.017.2666661
Produced by FastQC (version 0.11.5)