##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631339.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2274225
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.898619529729906	34.0	33.0	34.0	31.0	34.0
2	33.06715078763095	34.0	33.0	34.0	31.0	34.0
3	33.18112719717706	34.0	33.0	34.0	31.0	34.0
4	36.490050456749	37.0	37.0	37.0	35.0	37.0
5	36.461148303268146	37.0	37.0	37.0	35.0	37.0
6	36.50205894315646	37.0	37.0	37.0	35.0	37.0
7	36.50195121414988	37.0	37.0	37.0	35.0	37.0
8	36.48721696402071	37.0	37.0	37.0	35.0	37.0
9	38.349049016698	39.0	39.0	39.0	37.0	39.0
10	38.31489540392881	39.0	39.0	39.0	37.0	39.0
11	38.368438039332084	39.0	39.0	39.0	37.0	39.0
12	38.162329584803615	39.0	39.0	39.0	37.0	39.0
13	38.28254064571448	39.0	39.0	39.0	37.0	39.0
14	39.83164066879926	41.0	40.0	41.0	38.0	41.0
15	39.82946058547417	41.0	40.0	41.0	38.0	41.0
16	39.824383251437304	41.0	40.0	41.0	38.0	41.0
17	39.82056348866097	41.0	40.0	41.0	38.0	41.0
18	39.80285108113753	41.0	40.0	41.0	38.0	41.0
19	39.80619771570535	41.0	40.0	41.0	38.0	41.0
20	39.77249128824105	41.0	40.0	41.0	38.0	41.0
21	39.74242126438677	41.0	40.0	41.0	38.0	41.0
22	39.69979003836472	41.0	40.0	41.0	38.0	41.0
23	39.646409656036674	41.0	40.0	41.0	37.0	41.0
24	39.616570480053646	41.0	40.0	41.0	37.0	41.0
25	39.60712638371313	41.0	40.0	41.0	37.0	41.0
26	39.54802713012125	41.0	40.0	41.0	37.0	41.0
27	39.487834317184976	41.0	39.0	41.0	37.0	41.0
28	39.40858929965153	41.0	39.0	41.0	36.0	41.0
29	39.363195813958605	41.0	39.0	41.0	36.0	41.0
30	39.31559762116765	41.0	39.0	41.0	36.0	41.0
31	39.27119568204553	41.0	39.0	41.0	36.0	41.0
32	39.1943356528048	41.0	39.0	41.0	35.0	41.0
33	39.11159449922501	41.0	39.0	41.0	35.0	41.0
34	39.07269949103541	41.0	39.0	41.0	35.0	41.0
35	38.98754564741835	40.0	39.0	41.0	35.0	41.0
36	38.9331609669228	40.0	39.0	41.0	35.0	41.0
37	38.87104354230562	40.0	38.0	41.0	35.0	41.0
38	38.771318581055084	40.0	38.0	41.0	35.0	41.0
39	38.69809935252668	40.0	38.0	41.0	35.0	41.0
40	38.6129565016654	40.0	38.0	41.0	35.0	41.0
41	38.522315514076226	40.0	38.0	41.0	35.0	41.0
42	38.41061724323671	40.0	38.0	41.0	35.0	41.0
43	37.49900559531269	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	2.0
14	1.0
15	4.0
16	10.0
17	15.0
18	34.0
19	60.0
20	130.0
21	255.0
22	424.0
23	765.0
24	1247.0
25	1840.0
26	2791.0
27	4137.0
28	5848.0
29	8583.0
30	11487.0
31	15664.0
32	20751.0
33	28312.0
34	41722.0
35	61678.0
36	102634.0
37	197223.0
38	464895.0
39	1303707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45320933504821	16.74385779771131	11.120007914784157	27.682924952456332
2	20.134947069880948	19.07863118205103	32.88223460739373	27.904187140674296
3	21.013444140311535	20.22038276775605	27.53505040178522	31.231122690147195
4	15.40058701315833	14.17911596258066	31.785817146500456	38.634479877760555
5	16.984775033253086	33.93006408776616	31.66230254262441	17.42285833635634
6	37.17305895414921	31.924545724367643	14.92825028306346	15.974145038419682
7	32.43271883828557	27.819411008145632	19.129153887588078	20.61871626598072
8	29.362705976761315	30.80539524453385	18.702722905605206	21.129175873099626
9	28.351812155789336	13.119590190064748	18.0693203179105	40.459277336235424
10	18.66442414448878	24.681990568215546	29.915157910936692	26.73842737635898
11	39.06886961492377	20.02009475755477	19.23270564697864	21.67832998054282
12	24.724378634479876	23.531884488122326	26.236674031813035	25.50706284558476
13	32.27050973408524	17.11026833316844	23.505853642449626	27.113368290296698
14	24.686167815409647	18.67414174059295	22.239488177291165	34.400202266706245
15	27.39284811309347	25.663643658828832	19.793204278380546	27.150303949697154
16	27.6342490298893	23.477976013806902	21.654453715001814	27.233321241301983
17	26.414536820235462	24.241400916795833	22.741945058206642	26.60211720476206
18	27.33766447910827	21.927733623542085	23.957040310435424	26.777561586914224
19	26.813705767898956	23.113192406204313	23.313172619243918	26.759929206652817
20	26.82219217535644	22.543415888929196	23.603513284745354	27.030878650969008
21	28.243643438973713	22.349855445261575	23.74272554386659	25.663775571898118
22	28.11929338565885	22.104760962525695	22.667458145082392	27.108487506733063
23	26.58444085347756	22.198243357627323	23.65698204882982	27.560333740065296
24	26.582550099484443	22.82628148050435	23.20029900295705	27.39086941705416
25	27.14678626784949	22.59745627631391	23.188163000582616	27.067594455253985
26	27.145159339994944	23.080829733205817	22.934757994481636	26.839252932317603
27	27.55250689795425	22.10805878925788	23.205267728566874	27.134166584221
28	26.056656663258913	22.945530895140102	23.902428299750465	27.09538414185052
29	26.151414218030318	22.776022601105872	23.600215458013167	27.472347722850643
30	25.361562730160824	23.11196121755763	25.008387472655517	26.518088579626024
31	27.11134562323429	22.802009475755476	23.098286229374843	26.988358671635392
32	26.239971858545218	22.441315173300794	23.612263518341415	27.706449449812574
33	26.05239147401862	22.31665732282426	23.622200969561057	28.008750233596057
34	26.970110697050643	22.15317305895415	23.76119337356682	27.11552287042839
35	26.07187063725005	22.191559762116764	24.71228660312854	27.024282997504645
36	25.40056502764678	22.261473688839057	25.135331816333036	27.202629467181126
37	26.629643065220023	21.43288372962218	25.200760698699558	26.736712506458243
38	25.3399289867977	20.676230364189998	26.369378579516102	27.614462069496206
39	26.242698061977155	20.90523145247282	25.993910013301235	26.858160472248787
40	25.236728995591907	21.539557431652543	25.82919456078444	27.394519011971113
41	24.575404808231376	21.40513801404874	27.331332651782475	26.688124525937408
42	25.155206718772327	21.059305917400433	26.981147423847684	26.804339939979556
43	23.41619672635733	21.058910178192573	27.03488001407073	28.490013081379374
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	346.0
1	300.5
2	255.0
3	634.0
4	1013.0
5	1013.0
6	1175.5
7	1338.0
8	1161.5
9	985.0
10	1585.0
11	2185.0
12	2185.0
13	3562.5
14	4940.0
15	7148.5
16	9357.0
17	8387.0
18	7417.0
19	7417.0
20	8258.0
21	9099.0
22	7388.0
23	5677.0
24	6437.5
25	7198.0
26	7198.0
27	8527.0
28	9856.0
29	12235.0
30	14614.0
31	17841.5
32	21069.0
33	21069.0
34	27399.0
35	33729.0
36	43407.0
37	53085.0
38	64157.5
39	75230.0
40	75230.0
41	84295.0
42	93360.0
43	100346.0
44	107332.0
45	124007.5
46	140683.0
47	140683.0
48	155568.0
49	170453.0
50	176994.0
51	183535.0
52	190279.0
53	197023.0
54	197023.0
55	199205.5
56	201388.0
57	194447.0
58	187506.0
59	181578.0
60	175650.0
61	175650.0
62	167156.5
63	158663.0
64	145273.5
65	131884.0
66	116986.5
67	102089.0
68	102089.0
69	84775.0
70	67461.0
71	56729.0
72	45997.0
73	33737.5
74	21478.0
75	21478.0
76	15761.5
77	10045.0
78	8132.0
79	6219.0
80	5734.5
81	5250.0
82	5250.0
83	4500.0
84	3750.0
85	3491.0
86	3232.0
87	2842.5
88	2453.0
89	2453.0
90	1778.5
91	1104.0
92	636.0
93	168.0
94	122.5
95	77.0
96	77.0
97	51.5
98	26.0
99	16.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2274225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.0793003856495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.84403667516462	26.254994372767204
2	7.952230760826808	5.102039986251305
3	2.9090669457351823	2.7996249718260855
4	1.552462071205272	1.9920758847808604
5	0.9994064965815548	1.6030130605604642
6	0.6831113754494885	1.3148241005939003
7	0.4745318863926043	1.0655855648310077
8	0.38196693102777135	0.9802585534259634
9	0.3136916694669272	0.905670836397489
>10	2.1619612194629587	13.817643814445935
>50	0.3437205056533402	7.7551692411460165
>100	0.3331083620136952	21.746396253690918
>500	0.03661267512334742	7.794351992923357
>1k	0.014092425896533723	6.868351366359493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4847	0.2131275489452451	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4072	0.17905000604601568	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3477	0.15288724730402664	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3402	0.14958942057184316	No Hit
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG	2871	0.12624080730798404	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2871	0.12624080730798404	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2522	0.11089492024755686	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2311	0.1016170343743473	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2296	0.10095746902791061	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.397102309577988E-5	0.0
4	0.0	0.0	0.0	4.397102309577988E-5	0.0
5	0.0	0.0	0.0	4.397102309577988E-5	0.0
6	0.0	0.0	0.0	4.397102309577988E-5	0.0
7	0.0	0.0	0.0	4.397102309577988E-5	0.0
8	0.0	0.0	0.0	4.397102309577988E-5	0.0
9	0.0	0.0	0.0	8.794204619155977E-5	0.0
10	0.0	0.0	0.0	2.198551154788994E-4	0.0
11	0.0	0.0	0.0	3.5176818476623906E-4	0.0
12	0.0	0.0	0.0	5.276522771493586E-4	0.0
13	0.0	0.0	0.0	5.276522771493586E-4	0.0
14	0.0	0.0	0.0	6.595653464366982E-4	0.0
15	0.0	0.0	0.0	7.914784157240378E-4	0.0
16	0.0	4.397102309577988E-5	0.0	0.0014510437621607362	0.0
17	0.0	8.794204619155977E-5	0.0	0.002550319339555233	0.0
18	0.0	8.794204619155977E-5	0.0	0.0029900295705130317	0.0
19	0.0	8.794204619155977E-5	0.0	0.0032098846859919313	0.0
20	0.0	8.794204619155977E-5	0.0	0.0035176818476623903	0.0
21	0.0	8.794204619155977E-5	0.0	0.004001363101715969	0.0
22	0.0	8.794204619155977E-5	0.0	0.005452406863876705	0.0
23	0.0	8.794204619155977E-5	0.0	0.00712330574151634	0.0
24	0.0	8.794204619155977E-5	0.0	0.009937451219646252	0.0
25	0.0	8.794204619155977E-5	0.0	0.012136002374435247	0.0
26	0.0	8.794204619155977E-5	0.0	0.01820400356165287	0.0
27	0.0	8.794204619155977E-5	0.0	0.04700502368938869	0.0
28	0.0	8.794204619155977E-5	0.0	0.1497213336411305	0.0
29	0.0	8.794204619155977E-5	0.0	0.29082434675548813	0.0
30	0.0	8.794204619155977E-5	0.0	0.4771295716123075	0.0
31	0.0	8.794204619155977E-5	0.0	0.9277885873209555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTAGT	430	0.0	21.94186	27
CTAGACA	505	0.0	21.247524	4
ACATGTA	360	0.0	21.069445	8
TCTAGAC	495	0.0	20.929293	3
CGTAAGT	45	0.0038269435	20.555555	12
GTCTAGA	485	0.0	19.835052	1
TAGAACT	570	0.0	19.798246	4
GACATGT	400	0.0	19.425001	7
ACACGCA	105	2.2622771E-8	19.380953	37
AGTGCGC	585	0.0	19.2906	29
GGTATCA	2415	0.0	18.84472	1
GTCTATA	50	0.0070369476	18.5	1
AGTCGGT	450	0.0	18.088888	11
CCTATCG	420	0.0	18.059525	19
ATACACA	485	0.0	17.927835	37
GTCAAAA	540	0.0	17.814816	31
CTAGAAC	530	0.0	17.801888	3
ACGGAGT	325	0.0	17.646154	26
GTGCGCT	610	0.0	17.590162	30
GTTCTAG	600	0.0	17.266666	1
>>END_MODULE