FastQCFastQC Report
Fri 10 Feb 2017
ERR1631338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819918
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16390.19989803858434624No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14480.17660302615627416No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11940.14562431852941393No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11890.145014501450145No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10740.13098870862696027No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10160.12391483050744098No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC10010.12208537926963428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.12171948902207294No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT9790.1194021841208511No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG9200.11220634258547807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9060.11049885476352513No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC8980.10952314743669489No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG8870.10818154986230331No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC8700.10610817179278903No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC8580.10464461080254366No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG8480.10342497664400586No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG8300.10122963515863781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC303.5985856E-430.8333348
GACGGTT358.867218E-426.4285729
GGTATCA7400.023.01
ACTGATC2000.022.1999998
TAGTACT502.7011873E-422.1999994
AACGGAG2050.020.756125
ACGGAGT2050.020.756126
TCTAATA450.003824886420.5555552
CTAGCAC450.003824886420.5555553
ATGTAGG555.141365E-420.1818181
CATACAC555.141365E-420.1818183
GTAGACC1403.6379788E-1219.8214284
ACAACGG2300.019.30434823
CTTATAC10300.018.85922237
CAACGGA2400.018.524
GGATAAG701.2186564E-418.51
GAGCTAG500.00703320518.4999987
TTACAAC2450.018.12244821
TAGAACT3500.017.9714284
GTCTAGA2800.017.8392851
Produced by FastQC (version 0.11.5)