##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631338.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819918
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87446305606171	34.0	33.0	34.0	31.0	34.0
2	33.0363987618274	34.0	33.0	34.0	31.0	34.0
3	33.15053822455416	34.0	33.0	34.0	31.0	34.0
4	36.470442166167835	37.0	37.0	37.0	35.0	37.0
5	36.44357362565525	37.0	37.0	37.0	35.0	37.0
6	36.47928817271971	37.0	37.0	37.0	35.0	37.0
7	36.47515483255643	37.0	37.0	37.0	35.0	37.0
8	36.4579092055547	37.0	37.0	37.0	35.0	37.0
9	38.320316177959256	39.0	39.0	39.0	37.0	39.0
10	38.28529804199932	39.0	39.0	39.0	37.0	39.0
11	38.34215982573867	39.0	39.0	39.0	37.0	39.0
12	38.130543542159096	39.0	39.0	39.0	37.0	39.0
13	38.2478174646733	39.0	39.0	39.0	37.0	39.0
14	39.77907668815662	41.0	40.0	41.0	38.0	41.0
15	39.78391253759522	41.0	40.0	41.0	38.0	41.0
16	39.773717615664005	41.0	40.0	41.0	38.0	41.0
17	39.76774262792133	41.0	40.0	41.0	38.0	41.0
18	39.75072629214141	41.0	40.0	41.0	38.0	41.0
19	39.74699421161628	41.0	40.0	41.0	38.0	41.0
20	39.71627772533351	41.0	40.0	41.0	38.0	41.0
21	39.68334150488219	41.0	40.0	41.0	37.0	41.0
22	39.63313160584351	41.0	40.0	41.0	37.0	41.0
23	39.57089245509917	41.0	40.0	41.0	37.0	41.0
24	39.5311421386041	41.0	39.0	41.0	37.0	41.0
25	39.532202000687874	41.0	39.0	41.0	37.0	41.0
26	39.469790881527175	41.0	39.0	41.0	37.0	41.0
27	39.40539175868806	41.0	39.0	41.0	36.0	41.0
28	39.30870282150166	41.0	39.0	41.0	36.0	41.0
29	39.263376337633765	41.0	39.0	41.0	36.0	41.0
30	39.21239319053856	41.0	39.0	41.0	36.0	41.0
31	39.17901180361939	41.0	39.0	41.0	35.0	41.0
32	39.101624796626005	41.0	39.0	41.0	35.0	41.0
33	39.01139992047985	40.0	39.0	41.0	35.0	41.0
34	38.97384250620184	40.0	39.0	41.0	35.0	41.0
35	38.873960566788384	40.0	39.0	41.0	35.0	41.0
36	38.81682070646089	40.0	38.0	41.0	35.0	41.0
37	38.7593893535695	40.0	38.0	41.0	35.0	41.0
38	38.6631211901678	40.0	38.0	41.0	35.0	41.0
39	38.591575011159655	40.0	38.0	41.0	35.0	41.0
40	38.49215165418981	40.0	38.0	41.0	35.0	41.0
41	38.41001417214892	40.0	38.0	41.0	35.0	41.0
42	38.28638351640042	40.0	37.0	41.0	34.0	41.0
43	37.35710034418076	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	2.0
17	6.0
18	19.0
19	30.0
20	63.0
21	104.0
22	209.0
23	322.0
24	563.0
25	768.0
26	1156.0
27	1697.0
28	2331.0
29	3332.0
30	4536.0
31	6151.0
32	8196.0
33	11060.0
34	16026.0
35	23565.0
36	38681.0
37	73236.0
38	170391.0
39	457470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.92561207340246	17.38466529579787	11.550667261848135	27.13905536895153
2	20.233730690142185	19.339738851933973	32.59838178939845	27.828148668525387
3	20.80403162267446	20.347400593717907	27.948160669725507	30.90040711388212
4	15.249451774445738	14.428028168670526	32.22175876124197	38.10076129564176
5	17.27014164831117	33.70190677604346	31.51180971755712	17.516141858088247
6	36.75074824555627	32.62814086286677	14.929785661492978	15.691325230083983
7	32.055888515680834	28.237823782378236	19.35644783990594	20.349839862034987
8	28.939113423537478	30.500976926960988	19.096543800721534	21.46336584878
9	27.67654814261914	13.596237672547742	18.63149729607107	40.09571688876205
10	19.13874314260694	24.193516912666876	29.615888418110103	27.051851526616076
11	39.29905185640516	20.119207042655486	19.451579304271892	21.130161796667473
12	24.700640795786896	23.881900385160467	25.774406708963582	25.64305211008906
13	31.553643169194967	17.02243395071214	23.967030849426408	27.45689203066648
14	24.64563529523684	19.141792228003286	22.11452852602333	34.09804395073654
15	27.542510348595833	25.49413477933159	19.782466051483198	27.180888820589377
16	27.6463012154874	23.763839798614008	21.71217121712171	26.877687768776877
17	26.721208706236478	24.220470827570562	22.342722077085757	26.715598389107203
18	27.28528950456021	21.73619801004491	24.084115728646037	26.894396756748844
19	26.685473425391322	23.16134052429633	23.103042011518223	27.050144038794123
20	27.033800941069718	22.126602904192858	23.131825377659716	27.707770777077705
21	28.791903580601964	22.67543827553487	23.189270146526848	25.34338799733632
22	28.757144007083635	22.039277098441552	22.428096468183405	26.775482426291408
23	26.81121770713657	22.087208720872088	23.36904422149532	27.732529350496026
24	26.417519800760562	23.198417402715883	22.966199058930282	27.417863737593272
25	27.14698299098203	22.36735868708822	22.94485546115587	27.540802860773884
26	27.03002007517825	23.45258916135516	23.020472778985216	26.496917984481378
27	27.801926534116827	22.006103049329322	23.048158474384024	27.143811942169826
28	26.236160201385992	22.564695493939638	24.092653167755802	27.10649113691857
29	25.94539941799058	22.85472449683993	23.718469407916402	27.48140667725309
30	25.040674799187236	23.631387528996804	24.80369988218334	26.524237789632622
31	27.2235760161382	22.871433484811895	22.58079466483234	27.32419583421757
32	26.30994806797753	22.380652699416284	23.061818376959646	28.247580855646543
33	25.74964813554526	22.16916813632583	23.90970804397513	28.17147568415378
34	27.207598808661356	21.935242304718276	23.85616610441532	27.000992782205053
35	26.51350500903749	21.960732658631716	24.926760968779803	26.59900136355099
36	25.22984005717645	22.41797350466754	24.970667798487163	27.381518639668844
37	26.47813561843989	21.26688278583956	25.241182654850853	27.013798940869698
38	25.065067482357996	20.538273339529077	26.514236789532614	27.88242238858032
39	26.007966650323567	20.568032412997397	26.15310311518957	27.270897821489466
40	24.79918723579675	21.53300695923251	26.564973570527783	27.102832234442957
41	24.32292009688774	21.43019179966777	27.716308216187475	26.530579887257016
42	25.178493459102008	21.392749031000662	27.45433079893355	25.974426710963776
43	23.093894755329192	20.689264048356055	27.52543547037631	28.691405725938445
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	54.5
2	47.0
3	116.5
4	186.0
5	186.0
6	230.0
7	274.0
8	262.0
9	250.0
10	364.5
11	479.0
12	479.0
13	778.0
14	1077.0
15	1570.5
16	2064.0
17	1960.0
18	1856.0
19	1856.0
20	2116.5
21	2377.0
22	2031.5
23	1686.0
24	2025.5
25	2365.0
26	2365.0
27	2899.5
28	3434.0
29	4425.0
30	5416.0
31	6786.5
32	8157.0
33	8157.0
34	11097.5
35	14038.0
36	18036.5
37	22035.0
38	26297.5
39	30560.0
40	30560.0
41	33812.5
42	37065.0
43	39722.5
44	42380.0
45	48099.5
46	53819.0
47	53819.0
48	57628.5
49	61438.0
50	62986.5
51	64535.0
52	67410.5
53	70286.0
54	70286.0
55	69066.0
56	67846.0
57	66145.0
58	64444.0
59	62527.5
60	60611.0
61	60611.0
62	57654.5
63	54698.0
64	51304.0
65	47910.0
66	42405.0
67	36900.0
68	36900.0
69	30353.5
70	23807.0
71	20229.5
72	16652.0
73	12184.5
74	7717.0
75	7717.0
76	5865.0
77	4013.0
78	3283.5
79	2554.0
80	2436.0
81	2318.0
82	2318.0
83	1895.5
84	1473.0
85	1415.5
86	1358.0
87	1224.0
88	1090.0
89	1090.0
90	807.5
91	525.0
92	296.5
93	68.0
94	51.0
95	34.0
96	34.0
97	23.5
98	13.0
99	7.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	819918.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.56337287404486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.34579093643745	33.80786395236901
2	7.3875731750162865	5.9932977066495425
3	2.663471261592119	3.2411813376979146
4	1.4440409516864026	2.3430068627458462
5	0.8951500526275599	1.8155152681476296
6	0.6146476583668161	1.4959309291495022
7	0.4496224313009656	1.2766741633376981
8	0.3373003455048188	1.0945631748204903
9	0.268362698871047	0.9797126597812169
>10	2.071963867356735	16.972528258054147
>50	0.2849064118298827	8.083730192480555
>100	0.2178340394707523	17.064303025244524
>500	0.01722127635205598	4.782514803627745
>1k	0.0021148935870945935	1.0491776658942475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1639	0.19989803858434624	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1448	0.17660302615627416	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1194	0.14562431852941393	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1189	0.145014501450145	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1074	0.13098870862696027	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1016	0.12391483050744098	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1001	0.12208537926963428	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	998	0.12171948902207294	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	979	0.1194021841208511	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	920	0.11220634258547807	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.11049885476352513	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	898	0.10952314743669489	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	887	0.10818154986230331	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	870	0.10610817179278903	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	858	0.10464461080254366	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	848	0.10342497664400586	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	830	0.10122963515863781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.219634158537805E-4	0.0
10	0.0	0.0	0.0	1.219634158537805E-4	0.0
11	0.0	0.0	0.0	1.219634158537805E-4	0.0
12	0.0	0.0	0.0	1.219634158537805E-4	0.0
13	0.0	0.0	0.0	2.43926831707561E-4	0.0
14	0.0	0.0	0.0	2.43926831707561E-4	0.0
15	0.0	0.0	0.0	4.87853663415122E-4	0.0
16	0.0	0.0	0.0	8.537439109764635E-4	0.0
17	0.0	0.0	0.0	0.001219634158537805	0.0
18	0.0	0.0	0.0	0.001463560990245366	0.0
19	0.0	0.0	0.0	0.0018294512378067074	0.0
20	0.0	0.0	0.0	0.002195341485368049	0.0
21	0.0	0.0	0.0	0.00243926831707561	0.0
22	0.0	0.0	0.0	0.003414975643905854	0.0
23	0.0	0.0	0.0	0.005000500050005001	0.0
24	0.0	0.0	0.0	0.008293512278057073	0.0
25	0.0	0.0	0.0	0.010001000100010001	0.0
26	0.0	0.0	0.0	0.016343097724406588	0.0
27	0.0	0.0	0.0	0.04963911025248866	0.0
28	0.0	0.0	0.0	0.18099370912701027	0.0
29	0.0	2.43926831707561E-4	0.0	0.3613776011747516	0.0
30	0.0	2.43926831707561E-4	0.0	0.6000600060006	0.0
31	0.0	2.43926831707561E-4	0.0	1.1997541217536387	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGC	30	3.5985856E-4	30.833334	8
GACGGTT	35	8.867218E-4	26.42857	29
GGTATCA	740	0.0	23.0	1
ACTGATC	200	0.0	22.199999	8
TAGTACT	50	2.7011873E-4	22.199999	4
AACGGAG	205	0.0	20.7561	25
ACGGAGT	205	0.0	20.7561	26
TCTAATA	45	0.0038248864	20.555555	2
CTAGCAC	45	0.0038248864	20.555555	3
ATGTAGG	55	5.141365E-4	20.181818	1
CATACAC	55	5.141365E-4	20.181818	3
GTAGACC	140	3.6379788E-12	19.821428	4
ACAACGG	230	0.0	19.304348	23
CTTATAC	1030	0.0	18.859222	37
CAACGGA	240	0.0	18.5	24
GGATAAG	70	1.2186564E-4	18.5	1
GAGCTAG	50	0.007033205	18.499998	7
TTACAAC	245	0.0	18.122448	21
TAGAACT	350	0.0	17.971428	4
GTCTAGA	280	0.0	17.839285	1
>>END_MODULE