FastQCFastQC Report
Fri 10 Feb 2017
ERR1631336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763468
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89781.1759497451104697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70600.9247276899621203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63120.826753708079448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.32902492311400083No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA11360.1487947104528284No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8690.11382271424604568No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.10858346387798833No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA7670.10046262580749946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCA303.5983563E-430.83333411
CCAACCG551.901574E-523.54545420
AGCCGAC450.003824649620.55555510
CAAACGC450.003824649620.55555510
TATACTG450.003824649620.5555555
ATAATAC555.1409204E-420.1818183
GCAGTCG10400.018.855779
GGGCAGT10550.018.7630337
CAGTCGG10500.018.6761910
AGTCGGT10450.018.58851611
CCAATAC701.2185168E-418.53
CAACCGA609.232943E-418.521
TTAGACC500.00703277218.49999826
TACGGAG500.00703277218.4999985
CCGAAAT500.00703277218.49999824
GGCAGTC10700.018.3271038
GGTATCA39100.018.3107411
CTTATAC11450.017.93449837
ATTGACT1552.0008883E-1117.90322731
CTGGATA2000.017.57499923
Produced by FastQC (version 0.11.5)