##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631336.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	763468
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81251342557907	34.0	31.0	34.0	31.0	34.0
2	32.95987258143105	34.0	33.0	34.0	31.0	34.0
3	33.05110102846485	34.0	33.0	34.0	31.0	34.0
4	36.41008136555821	37.0	37.0	37.0	35.0	37.0
5	36.39748882729859	37.0	37.0	37.0	35.0	37.0
6	36.45884175892113	37.0	37.0	37.0	35.0	37.0
7	36.44822310823767	37.0	37.0	37.0	35.0	37.0
8	36.448499478694586	37.0	37.0	37.0	35.0	37.0
9	38.2881351936165	39.0	39.0	39.0	37.0	39.0
10	38.248015633923096	39.0	39.0	39.0	37.0	39.0
11	38.308390135539405	39.0	39.0	39.0	37.0	39.0
12	38.09642054414854	39.0	39.0	39.0	37.0	39.0
13	38.224325053571334	39.0	39.0	39.0	37.0	39.0
14	39.69537164622486	41.0	40.0	41.0	37.0	41.0
15	39.684966494993894	41.0	40.0	41.0	37.0	41.0
16	39.65070703683717	41.0	40.0	41.0	37.0	41.0
17	39.660560756966895	41.0	40.0	41.0	37.0	41.0
18	39.693254989076166	41.0	40.0	41.0	37.0	41.0
19	39.711094636579396	41.0	40.0	41.0	37.0	41.0
20	39.69269700891196	41.0	40.0	41.0	37.0	41.0
21	39.672149978781036	41.0	40.0	41.0	37.0	41.0
22	39.60452042521756	41.0	40.0	41.0	37.0	41.0
23	39.53230521776944	41.0	40.0	41.0	37.0	41.0
24	39.50138447190976	41.0	39.0	41.0	37.0	41.0
25	39.47503104255843	41.0	39.0	41.0	36.0	41.0
26	39.365136456275835	41.0	39.0	41.0	36.0	41.0
27	39.27247245464119	41.0	39.0	41.0	35.0	41.0
28	39.1498491095894	41.0	39.0	41.0	35.0	41.0
29	39.027638355504095	41.0	39.0	41.0	35.0	41.0
30	38.906914238710726	40.0	38.0	41.0	35.0	41.0
31	38.77476855611499	40.0	38.0	41.0	35.0	41.0
32	38.64246438619562	40.0	38.0	41.0	35.0	41.0
33	38.494991276648136	40.0	38.0	41.0	35.0	41.0
34	38.41509794778563	40.0	38.0	41.0	35.0	41.0
35	38.271657751208956	40.0	37.0	41.0	35.0	41.0
36	38.16128770295546	40.0	37.0	41.0	35.0	41.0
37	38.0404797581562	40.0	37.0	41.0	34.0	41.0
38	37.894545678404334	40.0	37.0	41.0	34.0	41.0
39	37.74304227551122	40.0	36.0	41.0	34.0	41.0
40	37.57386033206369	40.0	36.0	41.0	33.0	41.0
41	37.38502596048557	40.0	35.0	41.0	33.0	41.0
42	37.16994818381386	40.0	35.0	41.0	33.0	41.0
43	36.24407440783373	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	2.0
12	4.0
13	2.0
14	3.0
15	7.0
16	5.0
17	6.0
18	25.0
19	44.0
20	68.0
21	148.0
22	259.0
23	409.0
24	685.0
25	1077.0
26	1533.0
27	2263.0
28	3291.0
29	4592.0
30	6239.0
31	7758.0
32	9805.0
33	13009.0
34	18547.0
35	26384.0
36	41426.0
37	90308.0
38	154357.0
39	381206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1422561259935	18.009268233901093	13.174487994257781	24.67398764584763
2	19.269438928678085	21.460624413858863	33.47605924544316	25.79387741201989
3	20.112303331639307	23.236861269889506	29.94008917204126	26.710746226429922
4	13.889121744460803	16.609602498074576	33.876206992303544	35.62506876516108
5	14.733557922532444	36.93854883243306	32.06945150287897	16.258441742155533
6	31.80486935929207	34.644412077519945	17.226655210172527	16.32406335301545
7	29.02793568296248	31.27478820330387	20.250881503874428	19.446394609859222
8	30.14324110506269	30.384377603252528	19.327725589022723	20.144655702662064
9	28.397784844944386	13.439463081622282	19.65530971828551	38.50744235514782
10	19.33401268946439	26.24432196241362	28.830677906605125	25.590987441516866
11	35.5358705276449	23.045235687677806	19.52773397182331	21.891159812853978
12	23.275500741353927	27.628007984617557	26.38813938501679	22.70835188901172
13	31.464842010405153	18.99385435931827	24.77235457150791	24.768949058768673
14	23.47302048022969	21.591474691801096	25.038639471464425	29.896865356504794
15	27.602073695295676	26.66215217926619	22.037072935604375	23.69870118983376
16	24.667045639109954	25.904294613526694	24.49362645192726	24.93503329543609
17	22.736119915962423	27.23702892590128	25.4843686965269	24.542482461609396
18	23.59168950106619	23.569684649520347	27.970785939947714	24.867839909465754
19	23.548465685529717	26.133904760906812	27.892721109463658	22.424908444099817
20	24.713413004867263	23.819727873335882	28.012045036596163	23.454814085200688
21	26.745849203895904	24.112994912687892	26.810291983423014	22.33086389999319
22	25.84168557162841	24.161850922370025	26.088978189000716	23.90748531700084
23	23.87421607716368	24.560033950342387	26.89608470819995	24.669665264293986
24	23.395479574782442	25.605788323806628	27.313652962534118	23.685079138876812
25	23.358673840946835	24.662461295037904	26.88770190761106	25.091162956404194
26	23.815143529263832	25.62478060639084	27.004275228300333	23.555800636044992
27	24.185558530285487	24.607318184914103	26.499604436597213	24.7075188482032
28	22.600161368911337	25.379976632943357	28.087883185673796	23.93197881247151
29	22.555234797005244	25.62517355016844	28.483184625943718	23.336407026882593
30	22.124830379269333	26.367444346062964	28.937034689076686	22.570690585591013
31	23.186826428874557	26.41433563685708	26.925817454038675	23.47302048022969
32	22.086321889064113	25.882813687017663	28.042563669990102	23.988300753928126
33	21.886182525004322	25.053571335013388	29.006585737712648	24.053660402269646
34	23.198745723461887	25.28619405135513	27.653942273939446	23.861117951243536
35	22.935211429948605	25.663813021632865	28.08932397952501	23.311651568893524
36	21.448050212975527	26.174901895036857	28.08932397952501	24.287723912462607
37	22.03759686064118	25.105963838693963	28.62110789188283	24.23533140878203
38	21.638104020076806	23.766156538322498	30.00099545756993	24.594743984030764
39	21.85003169746473	23.545453116568083	30.212399209921042	24.392115976046146
40	21.0617864795905	23.761441212991244	30.522562831710037	24.654209475708218
41	19.79231611541021	23.91468928625692	31.915679504576485	24.377315093756387
42	20.428099147573967	23.736947717520575	31.562030104732614	24.272923030172844
43	18.945784237191344	23.52331728376304	32.126297369372395	25.40460110967323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	105.0
2	150.0
3	478.0
4	806.0
5	806.0
6	1106.0
7	1406.0
8	1463.5
9	1521.0
10	2512.0
11	3503.0
12	3503.0
13	5877.5
14	8252.0
15	12970.0
16	17688.0
17	15728.0
18	13768.0
19	13768.0
20	15009.0
21	16250.0
22	12364.5
23	8479.0
24	8546.5
25	8614.0
26	8614.0
27	9042.5
28	9471.0
29	11182.0
30	12893.0
31	14771.5
32	16650.0
33	16650.0
34	19375.5
35	22101.0
36	25107.5
37	28114.0
38	30721.5
39	33329.0
40	33329.0
41	35744.5
42	38160.0
43	40097.5
44	42035.0
45	44936.0
46	47837.0
47	47837.0
48	53170.0
49	58503.0
50	57890.5
51	57278.0
52	54876.0
53	52474.0
54	52474.0
55	49026.5
56	45579.0
57	43900.0
58	42221.0
59	40726.5
60	39232.0
61	39232.0
62	36454.5
63	33677.0
64	31146.5
65	28616.0
66	25517.0
67	22418.0
68	22418.0
69	19607.5
70	16797.0
71	14814.5
72	12832.0
73	10001.0
74	7170.0
75	7170.0
76	5812.0
77	4454.0
78	3842.5
79	3231.0
80	2961.0
81	2691.0
82	2691.0
83	2282.0
84	1873.0
85	1647.5
86	1422.0
87	1272.0
88	1122.0
89	1122.0
90	848.5
91	575.0
92	356.0
93	137.0
94	99.5
95	62.0
96	62.0
97	38.0
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	763468.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.98892624908322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.70144016487596	48.42884437498039
2	5.112785068625197	5.520675519948393
3	1.4389829444865097	2.3306743219071233
4	0.746853081931862	1.6128718383727925
5	0.49458314419446836	1.3351006447977423
6	0.3627589847948679	1.1750980845769456
7	0.24541858415755322	0.9274920088165596
8	0.19008014714918067	0.8209778436681602
9	0.15644296953386572	0.7601569149916306
>10	1.2662406217513773	13.947824981152673
>50	0.15952576407792524	6.028572286569712
>100	0.11830260614122706	11.839184432680721
>500	0.005366303783707831	1.848814921414563
>1k	4.878457985188937E-4	0.4804100600698287
>5k	7.317686977783406E-4	2.943301766052816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8978	1.1759497451104697	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7060	0.9247276899621203	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6312	0.826753708079448	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2512	0.32902492311400083	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1136	0.1487947104528284	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	869	0.11382271424604568	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	829	0.10858346387798833	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	767	0.10046262580749946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.3098125920143347E-4	0.0
3	0.0	0.0	0.0	1.3098125920143347E-4	0.0
4	0.0	0.0	0.0	1.3098125920143347E-4	0.0
5	0.0	0.0	0.0	1.3098125920143347E-4	0.0
6	0.0	0.0	0.0	1.3098125920143347E-4	0.0
7	0.0	0.0	0.0	1.3098125920143347E-4	0.0
8	0.0	0.0	0.0	1.3098125920143347E-4	0.0
9	0.0	0.0	0.0	2.6196251840286694E-4	0.0
10	0.0	0.0	0.0	3.929437776043004E-4	0.0
11	0.0	0.0	0.0	7.858875552086008E-4	0.0
12	0.0	0.0	0.0	7.858875552086008E-4	0.0
13	0.0	0.0	0.0	7.858875552086008E-4	0.0
14	0.0	0.0	0.0	0.0014407938512157681	0.0
15	0.0	0.0	0.0	0.001964718888021502	0.0
16	0.0	0.0	0.0	0.0037984565168415704	0.0
17	0.0	0.0	0.0	0.006418081700870239	0.0
18	0.0	0.0	0.0	0.006942006737675973	0.0
19	0.0	0.0	0.0	0.007989856811287442	0.0
20	0.0	0.0	0.0	0.009561631921704642	0.0
21	0.0	0.0	0.0	0.011788313328129012	0.0
22	0.0	0.0	0.0	0.016896582436984917	0.0
23	0.0	0.0	0.0	0.022397795323445123	1.3098125920143347E-4
24	1.3098125920143347E-4	0.0	0.0	0.03471003368837987	1.3098125920143347E-4
25	1.3098125920143347E-4	0.0	0.0	0.039556340278832904	1.3098125920143347E-4
26	1.3098125920143347E-4	0.0	0.0	0.05068974731095475	1.3098125920143347E-4
27	1.3098125920143347E-4	0.0	0.0	0.09325865655142063	1.3098125920143347E-4
28	1.3098125920143347E-4	0.0	0.0	0.2176908527927824	1.3098125920143347E-4
29	1.3098125920143347E-4	0.0	0.0	0.40368424085881793	1.3098125920143347E-4
30	1.3098125920143347E-4	0.0	0.0	0.6752083911833895	1.3098125920143347E-4
31	1.3098125920143347E-4	0.0	0.0	1.2884626467645008	1.3098125920143347E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACCA	30	3.5983563E-4	30.833334	11
CCAACCG	55	1.901574E-5	23.545454	20
AGCCGAC	45	0.0038246496	20.555555	10
CAAACGC	45	0.0038246496	20.555555	10
TATACTG	45	0.0038246496	20.555555	5
ATAATAC	55	5.1409204E-4	20.181818	3
GCAGTCG	1040	0.0	18.85577	9
GGGCAGT	1055	0.0	18.763033	7
CAGTCGG	1050	0.0	18.67619	10
AGTCGGT	1045	0.0	18.588516	11
CCAATAC	70	1.2185168E-4	18.5	3
CAACCGA	60	9.232943E-4	18.5	21
TTAGACC	50	0.007032772	18.499998	26
TACGGAG	50	0.007032772	18.499998	5
CCGAAAT	50	0.007032772	18.499998	24
GGCAGTC	1070	0.0	18.327103	8
GGTATCA	3910	0.0	18.310741	1
CTTATAC	1145	0.0	17.934498	37
ATTGACT	155	2.0008883E-11	17.903227	31
CTGGATA	200	0.0	17.574999	23
>>END_MODULE