FastQCFastQC Report
Fri 10 Feb 2017
ERR1631335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75179
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2310.30726665691216964No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1920.25539046808284227No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1590.21149523138110377No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1510.20085396187765203No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1440.19154285106213173No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1310.1742507881190226No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1240.1649396773035023No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC1140.1516380904241876No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT1120.14897777304832466No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1070.1423269796086673No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC1000.13301586879314703No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC980.13035555141728408No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT880.11705396453796939No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT880.11705396453796939No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT870.11572380585003791No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA870.11572380585003791No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG860.11439364716210644No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGC860.11439364716210644No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC840.1117333297862435No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC810.10774285372244909No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG800.10641269503451761No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT790.10508253634658614No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT790.10508253634658614No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG790.10508253634658614No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG780.10375237765865468No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG770.1024222189707232No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC770.1024222189707232No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG760.10109206028279173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303.56784E-430.8333341
CGCCTGG400.001914532423.12520
GAGCCGC400.001914532423.12516
TATTGGA450.003793127420.5555552
CGAGCCG450.003793127420.55555515
GCAATAC450.003793127420.55555537
CCGCCTG450.003793127420.55555519
TCAAAGC450.003793127420.5555553
GTATCAA656.793083E-519.9230771
AGCCGCC609.127785E-418.517
ATGGCTC609.127785E-418.518
TCTAGCG500.00697536318.528
GCGCAAT500.00697536318.535
CGCAATA500.00697536318.536
AAAGCAG609.127785E-418.55
CTAGCGG500.00697536318.529
CTTATAC1301.333683E-817.07692337
CAACGCA1101.4162417E-515.1363644
ATCAACG1101.4162417E-515.1363642
AACGCAG1101.4162417E-515.1363645
Produced by FastQC (version 0.11.5)