FastQCFastQC Report
Fri 10 Feb 2017
ERR1631334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23464
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2050.8736788271394477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.8012274122059324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1480.6307534947153085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.2514490282986703No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA390.16621206955335835No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.12359359018070235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGAC200.00180541737.03
CAGGCCG250.005388604529.65
GGTATCA953.311994E-617.5263141
AGTACAT1151.1204866E-616.08695612
GAGTACA1151.1204866E-616.08695611
GTACATG1804.366484E-813.3611111
TACATGG1804.366484E-813.3611112
CTCTTAT850.00905045213.05882337
ACATGGG1856.253322E-813.03
AACGCAG1754.6037894E-712.6857155
CAACGCA1754.6037894E-712.6857154
GTATCAA1359.1821916E-512.3333332
TCAACGC1859.030646E-712.03
CATGGGA1100.00356126311.7727274
CGCAGAG1901.2465734E-611.684217
ACGCAGA1951.7053189E-611.3846166
CAGAGTA1951.7053189E-611.3846169
AGAGTAC1951.7053189E-611.38461610
ATCAACG1951.7053189E-611.3846162
GCAGAGT2002.3130324E-611.18
Produced by FastQC (version 0.11.5)