##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23464
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71437095124446	34.0	31.0	34.0	31.0	34.0
2	32.87534094783498	34.0	31.0	34.0	31.0	34.0
3	32.94331742243437	34.0	33.0	34.0	31.0	34.0
4	36.344698261166045	37.0	37.0	37.0	35.0	37.0
5	36.30689566996249	37.0	37.0	37.0	35.0	37.0
6	36.35049437436072	37.0	37.0	37.0	35.0	37.0
7	36.36796795090351	37.0	37.0	37.0	35.0	37.0
8	36.358378793044665	37.0	37.0	37.0	35.0	37.0
9	38.18611489942039	39.0	39.0	39.0	37.0	39.0
10	38.17341459256734	39.0	38.0	39.0	37.0	39.0
11	38.2072962836686	39.0	39.0	39.0	37.0	39.0
12	37.99931810433004	39.0	38.0	39.0	37.0	39.0
13	38.119928400954656	39.0	39.0	39.0	37.0	39.0
14	39.58357483804978	41.0	39.0	41.0	37.0	41.0
15	39.56818956699625	41.0	39.0	41.0	37.0	41.0
16	39.51112342311626	41.0	39.0	41.0	37.0	41.0
17	39.49667575860893	41.0	39.0	41.0	37.0	41.0
18	39.51734572110467	41.0	39.0	41.0	37.0	41.0
19	39.51027105352881	41.0	39.0	41.0	37.0	41.0
20	39.48947323559496	41.0	39.0	41.0	37.0	41.0
21	39.46364643709512	41.0	39.0	41.0	37.0	41.0
22	39.394988066825775	41.0	39.0	41.0	36.0	41.0
23	39.28533071939993	41.0	39.0	41.0	36.0	41.0
24	39.20767984998295	41.0	39.0	41.0	36.0	41.0
25	39.172604841459254	41.0	39.0	41.0	35.0	41.0
26	39.0082679849983	40.0	39.0	41.0	35.0	41.0
27	38.909435731333105	40.0	39.0	41.0	35.0	41.0
28	38.85654619843164	40.0	38.0	41.0	35.0	41.0
29	38.665018752130926	40.0	38.0	41.0	35.0	41.0
30	38.51252983293556	40.0	38.0	41.0	35.0	41.0
31	38.35565121036481	40.0	37.0	41.0	35.0	41.0
32	38.1695789294238	40.0	37.0	41.0	34.0	41.0
33	37.999914763041254	40.0	37.0	41.0	34.0	41.0
34	37.868223661779744	40.0	36.0	41.0	33.0	41.0
35	37.68769178315718	40.0	36.0	41.0	33.0	41.0
36	37.485126150698946	40.0	35.0	41.0	33.0	41.0
37	37.33425673371974	39.0	35.0	41.0	33.0	41.0
38	37.09981247869076	39.0	35.0	41.0	33.0	41.0
39	36.88241561541084	39.0	35.0	41.0	33.0	41.0
40	36.66497613365155	39.0	35.0	41.0	32.0	41.0
41	36.421241050119335	38.0	35.0	41.0	32.0	41.0
42	36.16706443914081	38.0	35.0	41.0	31.0	41.0
43	35.03925161950222	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	3.0
21	10.0
22	11.0
23	21.0
24	23.0
25	35.0
26	90.0
27	94.0
28	129.0
29	161.0
30	243.0
31	301.0
32	324.0
33	485.0
34	700.0
35	959.0
36	1717.0
37	3419.0
38	5634.0
39	9104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.935731333106034	16.067166723491304	11.336515513126491	25.660586430276165
2	22.57927037163314	18.504943743607228	29.628366859870443	29.287419024889193
3	23.951585407432663	19.301909307875896	26.42771905898397	30.318786225707466
4	17.247698602113875	14.750255710876237	28.239004432321856	39.763041254688034
5	19.02488919195363	33.003750426184794	27.91510398908967	20.056256392771907
6	36.51977497442891	28.869757927037163	15.082679849982952	19.527787248550972
7	32.92703716331401	28.362598022502556	16.08421411524037	22.62615069894306
8	32.90572792362769	27.505966587112173	16.744800545516537	22.843504943743607
9	29.419536310944427	11.805318786225708	17.222127514490282	41.55301738833958
10	23.50835322195704	22.434367541766107	23.891919536310944	30.165359699965904
11	39.65649505625639	20.0818274803955	16.369757927037163	23.891919536310944
12	24.586600750085235	24.173201500170475	22.809410160245484	28.430787589498806
13	36.217183770883054	15.900954653937948	21.39021479713604	26.49164677804296
14	26.206102966246164	18.31316058643028	20.72962836685987	34.75110808046369
15	32.75230139788612	21.12598022502557	18.594442550289806	27.527275826798498
16	29.295942720763723	21.407262188885102	20.853221957040574	28.4435731333106
17	26.29560177292874	23.964370951244458	21.539379474940333	28.200647800886465
18	26.457551994544836	20.571939993181044	23.376235935901807	29.594272076372313
19	28.26457551994545	22.783839072621888	23.214285714285715	25.737299693146948
20	28.115410842141152	21.032219570405726	23.9345380156836	26.91783157176952
21	30.26764405046028	20.030685305148314	22.39601091033072	27.30565973406069
22	30.391237640640984	19.77071258097511	21.76525741561541	28.072792362768496
23	28.673712921922945	21.16433685646096	22.668769178315717	27.493181043300375
24	27.79151039890897	20.989601091033073	23.099215819979545	28.119672690078417
25	27.84265257415615	21.21547903170815	22.34486873508353	28.596999659052162
26	28.226218888510058	21.228264575519944	22.459938629389704	28.085577906580294
27	27.77872485509717	20.900102284350496	22.15308557790658	29.168087282645754
28	28.021650187521306	20.900102284350496	22.698602113876575	28.37964541425162
29	27.77872485509717	21.287930446641663	24.075178997613364	26.858165700647803
30	27.43351517217866	21.2836685987044	24.011251278554383	27.271564950562567
31	27.08404364132288	21.99965905216502	23.290998977156498	27.62529832935561
32	26.444766450733038	22.805148312308216	23.832253665189228	26.91783157176952
33	26.37231503579952	21.782304807364472	24.092226389362427	27.753153767473577
34	27.47613365155131	21.56495056256393	23.171667234913055	27.7872485509717
35	26.40640981929765	21.731162632117286	24.573815206273437	27.288612342311623
36	25.57961131946812	21.360381861575178	24.786907603136722	28.27309921581998
37	25.64353903852711	21.002386634844868	25.034094783498123	28.319979543129904
38	25.093760654619846	20.98107739515854	26.49164677804296	27.43351517217866
39	25.251449028298673	20.32475281281964	26.88799863620866	27.53579952267303
40	25.16195022161609	19.958233890214796	26.55557449710194	28.32424139106717
41	23.290998977156498	20.346062052505967	27.902318445277874	28.460620525059667
42	23.58506648482782	20.04347084896011	27.62529832935561	28.74616433685646
43	23.026764405046027	20.669962495738154	28.183600409137398	28.119672690078417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	10.5
4	18.0
5	18.0
6	26.5
7	35.0
8	32.0
9	29.0
10	50.5
11	72.0
12	72.0
13	136.5
14	201.0
15	302.5
16	404.0
17	354.0
18	304.0
19	304.0
20	350.5
21	397.0
22	282.0
23	167.0
24	149.5
25	132.0
26	132.0
27	126.0
28	120.0
29	141.5
30	163.0
31	169.0
32	175.0
33	175.0
34	180.0
35	185.0
36	208.0
37	231.0
38	298.5
39	366.0
40	366.0
41	407.5
42	449.0
43	551.5
44	654.0
45	800.0
46	946.0
47	946.0
48	1147.5
49	1349.0
50	1373.0
51	1397.0
52	1368.0
53	1339.0
54	1339.0
55	1348.0
56	1357.0
57	1522.0
58	1687.0
59	1720.5
60	1754.0
61	1754.0
62	1795.5
63	1837.0
64	1828.0
65	1819.0
66	1735.0
67	1651.0
68	1651.0
69	1547.5
70	1444.0
71	1265.5
72	1087.0
73	919.0
74	751.0
75	751.0
76	597.5
77	444.0
78	364.0
79	284.0
80	206.0
81	128.0
82	128.0
83	93.5
84	59.0
85	38.5
86	18.0
87	12.5
88	7.0
89	7.0
90	3.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23464.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.90487555404023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.36992145835353	83.83481077395159
2	2.942887617570057	5.173883395840436
3	0.6545137205468825	1.7260484145925674
4	0.3587704838553282	1.261506989430617
5	0.13090274410937652	0.5753494715308558
6	0.10181324541840395	0.5369928400954653
7	0.06787549694560263	0.41766109785202865
8	0.0484824978182876	0.34094783498124787
9	0.06302724716377388	0.498636208660075
>10	0.24241248909143798	3.077054210705762
>50	0.0048482497818287595	0.2514490282986703
>100	0.01454474934548628	2.305659734060689
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.8736788271394477	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	188	0.8012274122059324	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.6307534947153085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.2514490282986703	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	39	0.16621206955335835	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.12359359018070235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0042618479372655985	0.0
19	0.0	0.0	0.0	0.0042618479372655985	0.0
20	0.0	0.0	0.0	0.0042618479372655985	0.0
21	0.0	0.0	0.0	0.008523695874531197	0.0
22	0.0	0.0	0.0	0.008523695874531197	0.0
23	0.0	0.0	0.0	0.008523695874531197	0.0
24	0.0	0.0	0.0	0.008523695874531197	0.0
25	0.0	0.0	0.0	0.012785543811796795	0.0
26	0.0	0.0	0.0	0.05114217524718718	0.0
27	0.0	0.0	0.0	0.11506989430617115	0.0
28	0.0	0.0	0.0	0.36225707466757584	0.0
29	0.0	0.0	0.0	0.5881350153426526	0.0
30	0.0	0.0	0.0	0.8736788271394477	0.0
31	0.0	0.0	0.0	1.410671667234913	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGGAC	20	0.001805417	37.0	3
CAGGCCG	25	0.0053886045	29.6	5
GGTATCA	95	3.311994E-6	17.526314	1
AGTACAT	115	1.1204866E-6	16.086956	12
GAGTACA	115	1.1204866E-6	16.086956	11
GTACATG	180	4.366484E-8	13.361111	1
TACATGG	180	4.366484E-8	13.361111	2
CTCTTAT	85	0.009050452	13.058823	37
ACATGGG	185	6.253322E-8	13.0	3
AACGCAG	175	4.6037894E-7	12.685715	5
CAACGCA	175	4.6037894E-7	12.685715	4
GTATCAA	135	9.1821916E-5	12.333333	2
TCAACGC	185	9.030646E-7	12.0	3
CATGGGA	110	0.003561263	11.772727	4
CGCAGAG	190	1.2465734E-6	11.68421	7
ACGCAGA	195	1.7053189E-6	11.384616	6
CAGAGTA	195	1.7053189E-6	11.384616	9
AGAGTAC	195	1.7053189E-6	11.384616	10
ATCAACG	195	1.7053189E-6	11.384616	2
GCAGAGT	200	2.3130324E-6	11.1	8
>>END_MODULE