FastQCFastQC Report
Fri 10 Feb 2017
ERR1631333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34915
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA870.2491765716740656No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC700.2004868967492482No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC670.1918946011742804No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC670.1918946011742804No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC620.1775741085493341No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC530.15179722182443076No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT450.12888443362451668No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT450.12888443362451668No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC440.12602033509952743No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGC440.12602033509952743No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG420.12029213804954891No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT410.11742803952455964No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC410.11742803952455964No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCT380.10883574394959186No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC380.10883574394959186No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG370.1059716454246026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT200.001817592537.030
AACCCGT200.001817592537.029
GGGAGCT200.001817592537.07
GCCGGTC200.001817592537.08
TAACCCG200.001817592537.028
CGGTCCA200.001817592537.010
TATACAC250.00542453129.59999837
CTGGGGA250.00542453129.5999984
GGTCCAA250.00542453129.59999811
CCTCGCC250.00542453129.59999822
CCCGTTG250.00542453129.59999831
CGGGTAA250.00542453129.59999824
TGGGGAG250.00542453129.5999985
GTAACCC250.00542453129.59999827
CGCCGGT250.00542453129.5999987
CCGGTCC250.00542453129.5999989
GGTAACC250.00542453129.59999826
CGCCCCT400.001894121823.1250025
ATGTCCG502.6300852E-422.1999997
CTCTCGG450.003753075620.55555515
Produced by FastQC (version 0.11.5)