FastQCFastQC Report
Fri 10 Feb 2017
ERR1631332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5223182
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA103740.1986145610089788No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC92270.17665476715151798No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC71670.13721520712852817No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC67570.129365585958904No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT66820.12792967964738736No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT66460.12724044461785938No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC63040.1206927118373436No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC61060.11690191917493972No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG56950.10903315258782864No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC55220.10572099536259698No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC53600.10261943772972108No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC53460.10235140188490463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC11900.021.6092433
GGTATCA35700.021.24651
CTAGACA13500.020.5555574
GTTCTAG13650.020.3296721
GTCTAGA11900.019.4327721
ACATGTA11050.018.7511318
TAGAACT13850.018.2996394
CTCTAGT13250.017.73207527
TATACAC16800.017.72916837
CTTATAC59250.017.70379837
CTAGAAC14400.017.2152773
ACTGATC11350.016.7885468
ATACTAT1554.0199666E-1016.7096796
TCTAGTT13950.016.5770628
GACGGAC18900.016.5423287
CTAGTTT14250.016.2280729
ACGGAGT11150.016.0941726
ATAATAC2650.016.0566043
ACGGACC19250.016.0493498
AGACGGA19600.016.045926
Produced by FastQC (version 0.11.5)