##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631332.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5223182
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88798820335956	34.0	33.0	34.0	31.0	34.0
2	33.05611483574572	34.0	33.0	34.0	31.0	34.0
3	33.17128447754644	34.0	33.0	34.0	31.0	34.0
4	36.4851772348733	37.0	37.0	37.0	35.0	37.0
5	36.4686723533662	37.0	37.0	37.0	35.0	37.0
6	36.51159829391356	37.0	37.0	37.0	35.0	37.0
7	36.49685019591506	37.0	37.0	37.0	35.0	37.0
8	36.47801206237883	37.0	37.0	37.0	35.0	37.0
9	38.338067484533376	39.0	39.0	39.0	37.0	39.0
10	38.3005338508212	39.0	39.0	39.0	37.0	39.0
11	38.36042301417029	39.0	39.0	39.0	37.0	39.0
12	38.14062692052469	39.0	39.0	39.0	37.0	39.0
13	38.26466318807194	39.0	39.0	39.0	37.0	39.0
14	39.81420903962374	41.0	40.0	41.0	38.0	41.0
15	39.82398871033022	41.0	40.0	41.0	38.0	41.0
16	39.82329105131699	41.0	40.0	41.0	38.0	41.0
17	39.806180600254784	41.0	40.0	41.0	38.0	41.0
18	39.79195057725348	41.0	40.0	41.0	38.0	41.0
19	39.7998024958732	41.0	40.0	41.0	38.0	41.0
20	39.778411320915104	41.0	40.0	41.0	38.0	41.0
21	39.7470601254178	41.0	40.0	41.0	38.0	41.0
22	39.705396825153706	41.0	40.0	41.0	38.0	41.0
23	39.651339930333656	41.0	40.0	41.0	37.0	41.0
24	39.623947049901766	41.0	40.0	41.0	37.0	41.0
25	39.60535340334685	41.0	40.0	41.0	37.0	41.0
26	39.55201580186178	41.0	40.0	41.0	37.0	41.0
27	39.486396032150516	41.0	39.0	41.0	37.0	41.0
28	39.401129809376734	41.0	39.0	41.0	37.0	41.0
29	39.33696470848613	41.0	39.0	41.0	36.0	41.0
30	39.291173273303514	41.0	39.0	41.0	36.0	41.0
31	39.25919908592119	41.0	39.0	41.0	36.0	41.0
32	39.188585808420996	41.0	39.0	41.0	35.0	41.0
33	39.102582487073974	41.0	39.0	41.0	35.0	41.0
34	39.06600631569032	40.0	39.0	41.0	35.0	41.0
35	38.975068454440226	40.0	39.0	41.0	35.0	41.0
36	38.92516822121075	40.0	39.0	41.0	35.0	41.0
37	38.868425224317285	40.0	38.0	41.0	35.0	41.0
38	38.777501530676126	40.0	38.0	41.0	35.0	41.0
39	38.71273200895546	40.0	38.0	41.0	35.0	41.0
40	38.619930341313015	40.0	38.0	41.0	35.0	41.0
41	38.53654994216169	40.0	38.0	41.0	35.0	41.0
42	38.425143715076366	40.0	38.0	41.0	35.0	41.0
43	37.498589557093744	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	3.0
12	3.0
13	3.0
14	2.0
15	5.0
16	20.0
17	30.0
18	86.0
19	150.0
20	290.0
21	524.0
22	1050.0
23	1808.0
24	2845.0
25	4451.0
26	6492.0
27	9398.0
28	13520.0
29	18707.0
30	25703.0
31	34352.0
32	46090.0
33	63418.0
34	95215.0
35	142952.0
36	236595.0
37	450297.0
38	1110532.0
39	2958634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.465481003725316	16.993127943847256	11.503294351986968	27.038096700440462
2	20.03688556133024	19.306449593370477	32.830198143583736	27.826466701715546
3	20.127960312315366	20.32119501101053	28.311324399570992	31.239520277103118
4	14.548641039121362	14.512054146303921	33.137577821335725	37.80172699323899
5	16.73998723383562	33.74527251778705	32.301995220537975	17.21274502783935
6	36.61335178441035	33.38884228043365	15.179578272401766	14.818227662754238
7	31.95682248866687	28.588664917286057	19.634965812027993	19.819546782019085
8	28.789098292956282	31.430859579467075	19.003951997077642	20.776090130498996
9	27.463833349096394	13.96047466850667	18.267217186764697	40.30847479563224
10	18.455550658583217	24.046950690211446	30.32454162998724	27.172957021218103
11	39.126589883331654	20.232436855541316	19.88077382714215	20.760199433984877
12	24.854312945633524	23.617806157242846	26.262630710551537	25.265250186572093
13	31.243751414367715	17.186879568814565	24.173769935644593	27.39559908117312
14	24.46020452666593	18.73319750297807	22.393954489810998	34.412643480545
15	27.038173282110407	25.450673554932607	20.252979888504747	27.25817327445224
16	27.75338864316809	23.84586637034666	21.656224117788735	26.744520868696515
17	26.3987737743008	24.267276154650556	22.605281608031273	26.728668463017367
18	27.169357682730567	22.153813518272962	24.016490330989807	26.660338468006668
19	26.74509523122112	23.163561981948934	23.112482008093917	26.978860778736024
20	26.987648525362506	22.41643120994061	23.145833325356076	27.450086939340807
21	28.777189843279444	22.802709153156066	23.07878607331699	25.3413149302475
22	28.240218319024684	22.342223571761426	22.546926375531235	26.87063173368265
23	26.491839648704563	22.22827004687947	23.603064185777942	27.676826118638026
24	26.27090153090587	23.323770835479216	23.06546086274612	27.33986677086879
25	27.021440187226865	22.427631279170438	23.19901929513465	27.351909238468046
26	27.139567413120968	23.511663962695536	23.109035832946276	26.23973279123722
27	27.55104072574917	22.262961543365712	23.094963951093415	27.091033779791708
28	26.300174874243325	22.543729090810928	24.00797062020814	27.148125414737606
29	25.791251386606863	22.89183107155753	23.958422279752075	27.358495262083533
30	25.163205111366977	23.793446217267558	24.53230616892155	26.51104250244391
31	27.139969466888193	23.088377927477925	22.668327467815597	27.103325137818292
32	26.16215555958035	22.623412318391356	23.1290045033851	28.085427618643195
33	25.69052734520834	22.232405457056636	24.154815972332575	27.922251225402444
34	27.122738591149993	21.94629633813258	23.885344221204623	27.045620849512808
35	26.781911869048407	22.07074155179735	24.779473508677277	26.36787307047696
36	25.56083628715216	22.727467662432595	24.617656440078097	27.094039610337145
37	26.640599542577686	21.57952757533626	24.918469239632085	26.86140364245397
38	25.221541198449525	21.075620187081363	26.09909055437854	27.60374806009057
39	26.0470150188142	20.79014286693437	25.87977979706623	27.2830623171852
40	25.174615780189164	21.76625283208588	26.283384343107326	26.77574704461763
41	24.472897938459738	21.462032148219226	27.47742659551208	26.58764331780895
42	25.394252009598745	21.51125501657802	27.18731608433327	25.907176889489968
43	23.450838971339692	20.731500453172032	27.429639633464813	28.388020942023463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	80.0
2	100.0
3	297.5
4	495.0
5	495.0
6	666.5
7	838.0
8	807.0
9	776.0
10	1147.0
11	1518.0
12	1518.0
13	2385.5
14	3253.0
15	5324.0
16	7395.0
17	7112.0
18	6829.0
19	6829.0
20	8126.0
21	9423.0
22	9297.0
23	9171.0
24	11430.5
25	13690.0
26	13690.0
27	17883.0
28	22076.0
29	29142.5
30	36209.0
31	46023.5
32	55838.0
33	55838.0
34	75682.0
35	95526.0
36	123583.0
37	151640.0
38	182099.0
39	212558.0
40	212558.0
41	232682.0
42	252806.0
43	272695.0
44	292584.0
45	320636.5
46	348689.0
47	348689.0
48	373606.5
49	398524.0
50	411877.5
51	425231.0
52	440437.5
53	455644.0
54	455644.0
55	441072.0
56	426500.0
57	410938.0
58	395376.0
59	386412.5
60	377449.0
61	377449.0
62	355867.5
63	334286.0
64	309916.0
65	285546.0
66	256665.0
67	227784.0
68	227784.0
69	186115.0
70	144446.0
71	122699.5
72	100953.0
73	75477.0
74	50001.0
75	50001.0
76	38698.5
77	27396.0
78	22159.5
79	16923.0
80	15059.5
81	13196.0
82	13196.0
83	10660.5
84	8125.0
85	7384.5
86	6644.0
87	5635.0
88	4626.0
89	4626.0
90	3575.5
91	2525.0
92	1430.0
93	335.0
94	235.0
95	135.0
96	135.0
97	92.5
98	50.0
99	31.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5223182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.40586719293576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.67530024841932	28.917848335386875
2	8.870045804786706	6.281033275190702
3	2.928527353138638	3.110611516083189
4	1.4845037605176976	2.1024057196921326
5	0.9268753889186356	1.6408413462226947
6	0.6399137224800863	1.359402016384025
7	0.4711936986868275	1.16781150624978
8	0.3503773872638605	0.9924332192697652
9	0.2792683941770848	0.889896570787647
>10	1.921238774433318	13.225343734965453
>50	0.2159807482776877	5.363916412346911
>100	0.19078342157425843	13.954552722526792
>500	0.026303985009766617	6.412288937584947
>1k	0.01892802203399003	12.829800834539506
>5k	7.0505526201109E-4	1.5526083708656497
>10k+	5.4235020154699224E-5	0.19920548190415252
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	10374	0.1986145610089788	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	9227	0.17665476715151798	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	7167	0.13721520712852817	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	6757	0.129365585958904	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	6682	0.12792967964738736	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6646	0.12724044461785938	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	6304	0.1206927118373436	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	6106	0.11690191917493972	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	5695	0.10903315258782864	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	5522	0.10572099536259698	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	5360	0.10261943772972108	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	5346	0.10235140188490463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.914541748688826E-5	0.0	0.0	0.0
2	0.0	3.829083497377652E-5	0.0	1.914541748688826E-5	0.0
3	0.0	3.829083497377652E-5	0.0	3.829083497377652E-5	0.0
4	0.0	5.7436252460664784E-5	0.0	7.658166994755304E-5	0.0
5	0.0	1.3401792240821782E-4	0.0	7.658166994755304E-5	0.0
6	0.0	1.3401792240821782E-4	0.0	7.658166994755304E-5	0.0
7	0.0	1.3401792240821782E-4	0.0	7.658166994755304E-5	0.0
8	0.0	1.3401792240821782E-4	0.0	7.658166994755304E-5	0.0
9	0.0	1.3401792240821782E-4	0.0	9.57270874344413E-5	0.0
10	0.0	1.3401792240821782E-4	0.0	1.1487250492132957E-4	0.0
11	0.0	1.3401792240821782E-4	0.0	1.1487250492132957E-4	0.0
12	0.0	1.3401792240821782E-4	0.0	1.3401792240821782E-4	0.0
13	0.0	1.3401792240821782E-4	0.0	1.3401792240821782E-4	0.0
14	0.0	1.3401792240821782E-4	0.0	1.3401792240821782E-4	0.0
15	0.0	1.3401792240821782E-4	0.0	2.1059959235577086E-4	0.0
16	0.0	1.3401792240821782E-4	0.0	3.446175147639887E-4	0.0
17	0.0	1.3401792240821782E-4	0.0	4.786354371722065E-4	0.0
18	0.0	1.7230875738199434E-4	0.0	5.552171071197596E-4	0.0
19	0.0	1.7230875738199434E-4	0.0	5.935079420935361E-4	0.0
20	0.0	1.7230875738199434E-4	0.0	8.998346218837482E-4	1.914541748688826E-5
21	0.0	1.7230875738199434E-4	0.0	0.0011870158841870721	1.914541748688826E-5
22	0.0	1.7230875738199434E-4	0.0	0.001474197146490396	1.914541748688826E-5
23	0.0	2.488904273295474E-4	0.0	0.0023165955159134798	1.914541748688826E-5
24	0.0	2.488904273295474E-4	0.0	0.003886519749838317	1.914541748688826E-5
25	0.0	2.488904273295474E-4	0.0	0.005322426061354937	1.914541748688826E-5
26	0.0	2.488904273295474E-4	0.0	0.0088068920439686	1.914541748688826E-5
27	0.0	2.488904273295474E-4	0.0	0.030670958813994995	1.914541748688826E-5
28	0.0	2.6803584481643563E-4	0.0	0.1308206376879075	3.829083497377652E-5
29	0.0	3.2547209727710043E-4	0.0	0.28090156536762456	3.829083497377652E-5
30	0.0	3.446175147639887E-4	0.0	0.4931476636272678	3.829083497377652E-5
31	0.0	3.446175147639887E-4	0.0	1.0521172725744574	3.829083497377652E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	1190	0.0	21.609243	3
GGTATCA	3570	0.0	21.2465	1
CTAGACA	1350	0.0	20.555557	4
GTTCTAG	1365	0.0	20.329672	1
GTCTAGA	1190	0.0	19.432772	1
ACATGTA	1105	0.0	18.751131	8
TAGAACT	1385	0.0	18.299639	4
CTCTAGT	1325	0.0	17.732075	27
TATACAC	1680	0.0	17.729168	37
CTTATAC	5925	0.0	17.703798	37
CTAGAAC	1440	0.0	17.215277	3
ACTGATC	1135	0.0	16.788546	8
ATACTAT	155	4.0199666E-10	16.709679	6
TCTAGTT	1395	0.0	16.57706	28
GACGGAC	1890	0.0	16.542328	7
CTAGTTT	1425	0.0	16.22807	29
ACGGAGT	1115	0.0	16.09417	26
ATAATAC	265	0.0	16.056604	3
ACGGACC	1925	0.0	16.049349	8
AGACGGA	1960	0.0	16.04592	6
>>END_MODULE