FastQCFastQC Report
Fri 10 Feb 2017
ERR1631331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6868201
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219790.32001101889708816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185570.27018720040371563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT166010.24170812706267625No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT119220.17358257278725536No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA111540.16240060534046688No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT96350.14028418795547773No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC94660.1378235727230464No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC94610.1377507734558147No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC92410.1345476056976201No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC84580.12314724044913652No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC84400.12288516308710243No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG81950.11931799899274935No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG78840.11478988457093786No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC77170.11235838904539923No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG75720.11024721029568003No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA75620.11010161176121666No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA73130.1064762082530782No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA71880.10465622657228582No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC71880.10465622657228582No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG71840.10459798715850045No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC71620.104277670382681No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG70380.10247224855533493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98100.018.9148831
CTTATAC65850.018.54214337
AGTCGGT27300.017.1446911
GCAGTCG27850.016.938969
AAGACGG30950.016.9159955
CTCTATG43850.016.6225781
CAAGACG32700.016.293584
CCGCTTA8750.016.2825
TCGGTGA29250.016.12820413
CAGTCGG30250.015.900825510
TCGTTTA24400.015.77049330
TCTATGG46500.015.5161292
CGCTTAT9600.015.22395926
ATACACA15750.015.15238137
CGCCTTA20750.015.0674725
CGCAAGA34050.015.0499272
AGACGGA34200.015.0380126
GACGTAT1601.0993972E-815.031251
CGGTGAT30900.014.96763714
TCTTATA108200.014.89232837
Produced by FastQC (version 0.11.5)