##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631331.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6868201
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86475643330765	34.0	33.0	34.0	31.0	34.0
2	33.02925919028869	34.0	33.0	34.0	31.0	34.0
3	33.14359233225702	34.0	33.0	34.0	31.0	34.0
4	36.47085241098797	37.0	37.0	37.0	35.0	37.0
5	36.452904916440275	37.0	37.0	37.0	35.0	37.0
6	36.494975467374935	37.0	37.0	37.0	35.0	37.0
7	36.47697861492405	37.0	37.0	37.0	35.0	37.0
8	36.46511859510227	37.0	37.0	37.0	35.0	37.0
9	38.318823226052935	39.0	39.0	39.0	37.0	39.0
10	38.2776061737273	39.0	39.0	39.0	37.0	39.0
11	38.335925229911005	39.0	39.0	39.0	37.0	39.0
12	38.108657856693476	39.0	39.0	39.0	37.0	39.0
13	38.24031795225562	39.0	39.0	39.0	37.0	39.0
14	39.76433479451169	41.0	40.0	41.0	38.0	41.0
15	39.771088382532774	41.0	40.0	41.0	38.0	41.0
16	39.75300460775682	41.0	40.0	41.0	38.0	41.0
17	39.73901142380661	41.0	40.0	41.0	38.0	41.0
18	39.72417391395505	41.0	40.0	41.0	38.0	41.0
19	39.732659396543575	41.0	40.0	41.0	38.0	41.0
20	39.714578679336846	41.0	40.0	41.0	38.0	41.0
21	39.67831663633606	41.0	40.0	41.0	37.0	41.0
22	39.628082229975504	41.0	40.0	41.0	37.0	41.0
23	39.54960127113345	41.0	40.0	41.0	37.0	41.0
24	39.51948217590021	41.0	39.0	41.0	37.0	41.0
25	39.500411388659124	41.0	39.0	41.0	37.0	41.0
26	39.42876569861598	41.0	39.0	41.0	36.0	41.0
27	39.35093527402591	41.0	39.0	41.0	36.0	41.0
28	39.23678500381686	41.0	39.0	41.0	36.0	41.0
29	39.150143101519596	41.0	39.0	41.0	35.0	41.0
30	39.0858473710947	41.0	39.0	41.0	35.0	41.0
31	39.02880827162746	40.0	39.0	41.0	35.0	41.0
32	38.940302416891996	40.0	39.0	41.0	35.0	41.0
33	38.8215588041177	40.0	38.0	41.0	35.0	41.0
34	38.77814146673925	40.0	38.0	41.0	35.0	41.0
35	38.654841639025996	40.0	38.0	41.0	35.0	41.0
36	38.58750406401909	40.0	38.0	41.0	35.0	41.0
37	38.50974279873289	40.0	38.0	41.0	35.0	41.0
38	38.402767041908064	40.0	38.0	41.0	35.0	41.0
39	38.30736287420825	40.0	37.0	41.0	34.0	41.0
40	38.186690517647925	40.0	37.0	41.0	34.0	41.0
41	38.0841764823132	40.0	37.0	41.0	34.0	41.0
42	37.943542712276475	40.0	37.0	41.0	34.0	41.0
43	36.99468143113459	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	7.0
11	6.0
12	13.0
13	8.0
14	12.0
15	23.0
16	39.0
17	90.0
18	174.0
19	310.0
20	655.0
21	1195.0
22	1950.0
23	3272.0
24	5130.0
25	7694.0
26	11066.0
27	15899.0
28	22164.0
29	30462.0
30	41024.0
31	53863.0
32	70514.0
33	95480.0
34	145295.0
35	216078.0
36	354793.0
37	687308.0
38	1460478.0
39	3643196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16055965747071	17.43679312821509	11.63897212676216	26.76367508755204
2	20.10487171240329	19.697224935612688	32.10143383980754	28.09646951217648
3	20.235954072980682	21.11995557497516	28.621628866132486	30.022461485911666
4	14.467092620032524	14.892051062570824	33.127277434076255	37.51357888332039
5	16.72347678817204	34.28886254202519	31.798486969149565	17.1891737006532
6	35.755505699381835	33.387447455308894	15.541595244518907	15.315451600790366
7	31.355940223648084	29.10424432831829	19.73535719178865	19.804458256244974
8	29.860716073976285	29.877095909103417	19.010844324445365	21.25134369247493
9	28.048028879760505	13.251796212720041	18.520832456708824	40.17934245081063
10	19.10343043251064	24.057478807041317	29.445454493833246	27.393636266614795
11	39.32858109423414	20.21893360430191	19.48258357610676	20.969901725357193
12	24.41340898439053	24.18760021729125	25.992832766542506	25.406158031775718
13	31.64121143222221	16.98067659930162	23.645100077880656	27.733011890595517
14	24.4029695694695	19.418665819477326	22.34311430314867	33.8352503079045
15	27.705726725237074	25.635533962969344	19.905125082972965	26.753614228820616
16	27.648142504856803	23.815639641297626	21.97879765021437	26.5574202036312
17	26.318434769162984	24.11694124851617	22.90524112500493	26.65938285731591
18	26.912156472997808	21.713822877344448	24.571383394283306	26.80263725537444
19	26.62229891058809	23.434986832796536	23.69228856290024	26.250425693715133
20	27.00273332128748	21.95324510741605	23.446445437458806	27.597576133837663
21	29.097168239543368	22.688517706456174	22.983398418304883	25.230915635695577
22	28.48523215904718	22.114728441989396	22.82912221118747	26.570917187775954
23	26.4724925784787	22.027980835156104	23.71706943346591	27.78245715289928
24	26.228833431054216	23.341177697041772	23.510115676579645	26.919873195324367
25	26.621075882898595	22.10699715980939	23.40936149073098	27.86256546656104
26	26.554653831476394	23.703164773424657	23.571281620907715	26.17089977419123
27	27.510959565685393	22.16606648524119	23.18726548626052	27.135708462812897
28	25.88219244020377	22.84426445877166	24.484009713751824	26.789533387272733
29	25.359668419721554	23.217084066118623	24.696321496706343	26.72692601745348
30	24.94402828338891	24.036003023207968	24.850058989246236	26.16990970415688
31	26.640542406956346	23.58300230293202	22.781249413055907	26.99520587705572
32	25.589976181535746	22.943009384844736	23.513537242139535	27.95347719147998
33	25.032930748532255	22.3640804921114	24.724422013857776	27.878566745498567
34	26.67531133698621	22.43053166324049	24.379324367472645	26.514832632300656
35	26.628472288449334	22.49115889299105	25.104244910712424	25.776123907847193
36	24.622968955043685	23.67912645538475	24.728848209305465	26.9690563802661
37	25.633772220702333	22.39506386024521	25.222980515567322	26.748183403485132
38	24.6893327670521	21.625357207804488	26.500898852552513	27.1844111725909
39	25.340900768629222	21.142144791627384	26.2141862184872	27.30276822125619
40	24.59074799936694	21.957161707993112	27.014745200380712	26.43734509225924
41	23.39283605706938	21.946620374097964	28.21060420334233	26.449939365490323
42	24.525811052996264	22.118688722126798	27.932015967500078	25.42348425737686
43	22.971663176427132	20.89451080421205	28.264970696110964	27.868855323249857
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	175.0
1	252.0
2	329.0
3	1139.0
4	1949.0
5	1949.0
6	2614.0
7	3279.0
8	3165.0
9	3051.0
10	4504.5
11	5958.0
12	5958.0
13	10932.0
14	15906.0
15	27805.5
16	39705.0
17	36720.0
18	33735.0
19	33735.0
20	37862.5
21	41990.0
22	33813.5
23	25637.0
24	29237.0
25	32837.0
26	32837.0
27	39005.5
28	45174.0
29	55486.5
30	65799.0
31	79224.5
32	92650.0
33	92650.0
34	116245.5
35	139841.0
36	174453.5
37	209066.0
38	240992.0
39	272918.0
40	272918.0
41	297149.0
42	321380.0
43	337619.0
44	353858.0
45	382620.5
46	411383.0
47	411383.0
48	448900.5
49	486418.0
50	497158.5
51	507899.0
52	519798.0
53	531697.0
54	531697.0
55	525155.5
56	518614.0
57	510187.0
58	501760.0
59	496616.0
60	491472.0
61	491472.0
62	459836.0
63	428200.0
64	396628.5
65	365057.0
66	327253.5
67	289450.0
68	289450.0
69	250073.5
70	210697.0
71	184635.0
72	158573.0
73	122853.5
74	87134.0
75	87134.0
76	69896.0
77	52658.0
78	44756.0
79	36854.0
80	33328.0
81	29802.0
82	29802.0
83	24079.0
84	18356.0
85	17326.5
86	16297.0
87	14701.5
88	13106.0
89	13106.0
90	9666.0
91	6226.0
92	3552.5
93	879.0
94	588.0
95	297.0
96	297.0
97	204.0
98	111.0
99	67.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6868201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.894068407366866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.02141055119777	34.78104652907968
2	9.508368443756218	7.966884760503428
3	2.797375849548008	3.515803656062407
4	1.3260430841101016	2.222133587072973
5	0.7202659722877807	1.5087435957261444
6	0.47775806452528	1.2009137422435963
7	0.327868391184316	0.9615018566222338
8	0.2552619438583492	0.8555169070239298
9	0.20035633688842316	0.7554367875107737
>10	1.122105745252691	8.89581631903564
>50	0.1069762009049737	3.144143831129954
>100	0.09984590484594304	8.997925135882927
>500	0.01703454619124043	4.982080570404709
>1k	0.017555984805144087	14.687199815391205
>5k	0.0015991590119469868	4.356615480816934
>10k+	1.7382163173336812E-4	1.1682374254936574
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21979	0.32001101889708816	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18557	0.27018720040371563	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16601	0.24170812706267625	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	11922	0.17358257278725536	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	11154	0.16240060534046688	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	9635	0.14028418795547773	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	9466	0.1378235727230464	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	9461	0.1377507734558147	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	9241	0.1345476056976201	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	8458	0.12314724044913652	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	8440	0.12288516308710243	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	8195	0.11931799899274935	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	7884	0.11478988457093786	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	7717	0.11235838904539923	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	7572	0.11024721029568003	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	7562	0.11010161176121666	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	7313	0.1064762082530782	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	7188	0.10465622657228582	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	7188	0.10465622657228582	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	7184	0.10459798715850045	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	7162	0.104277670382681	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	7038	0.10247224855533493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.91197068926783E-5
2	0.0	1.455985344633915E-5	0.0	0.0	2.91197068926783E-5
3	0.0	1.455985344633915E-5	0.0	0.0	4.3679560339017454E-5
4	0.0	1.455985344633915E-5	0.0	1.455985344633915E-5	4.3679560339017454E-5
5	0.0	8.735912067803491E-5	0.0	1.455985344633915E-5	4.3679560339017454E-5
6	0.0	8.735912067803491E-5	0.0	1.455985344633915E-5	5.82394137853566E-5
7	0.0	8.735912067803491E-5	0.0	2.91197068926783E-5	5.82394137853566E-5
8	0.0	8.735912067803491E-5	0.0	2.91197068926783E-5	1.0191897412437406E-4
9	0.0	8.735912067803491E-5	0.0	2.91197068926783E-5	1.0191897412437406E-4
10	0.0	1.0191897412437406E-4	0.0	8.735912067803491E-5	1.0191897412437406E-4
11	0.0	1.164788275707132E-4	0.0	8.735912067803491E-5	1.0191897412437406E-4
12	0.0	1.164788275707132E-4	0.0	1.164788275707132E-4	1.0191897412437406E-4
13	0.0	1.164788275707132E-4	0.0	1.3103868101705236E-4	1.164788275707132E-4
14	0.0	1.164788275707132E-4	0.0	1.8927809480240895E-4	1.164788275707132E-4
15	0.0	1.164788275707132E-4	0.0	2.7663721548044386E-4	1.455985344633915E-4
16	0.0	1.455985344633915E-4	0.0	5.678342844072269E-4	1.7471824135606982E-4
17	0.0	1.6015838790973066E-4	0.0	9.172707671193665E-4	1.7471824135606982E-4
18	0.0	1.7471824135606982E-4	0.0	0.0011065488619217754	1.8927809480240895E-4
19	0.0	1.7471824135606982E-4	0.0	0.0013249466636168627	1.8927809480240895E-4
20	0.0	1.7471824135606982E-4	0.0	0.0017035028532216806	2.1839780169508724E-4
21	0.0	1.7471824135606982E-4	0.0	0.0022567772841825683	3.057569223731222E-4
22	0.0	1.7471824135606982E-4	0.0	0.003188607904748274	3.057569223731222E-4
23	0.0	2.038379482487481E-4	0.0	0.00497946987864799	3.057569223731222E-4
24	0.0	2.4751750858776554E-4	0.0	0.0072362471628305575	3.203167758194613E-4
25	0.0	2.4751750858776554E-4	0.0	0.00875047192124983	3.203167758194613E-4
26	0.0	2.4751750858776554E-4	0.0	0.01269619220520774	3.3487662926580045E-4
27	0.0	2.620773620341047E-4	0.0	0.031303684909629176	3.4943648271213964E-4
28	0.0	3.203167758194613E-4	0.0	0.11577995460528892	3.4943648271213964E-4
29	0.0	3.3487662926580045E-4	0.0	0.2366704177702429	3.4943648271213964E-4
30	0.0	3.3487662926580045E-4	0.0	0.39760047791262954	3.4943648271213964E-4
31	0.0	3.3487662926580045E-4	0.0	0.8939167621914385	3.6399633615847877E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9810	0.0	18.914883	1
CTTATAC	6585	0.0	18.542143	37
AGTCGGT	2730	0.0	17.14469	11
GCAGTCG	2785	0.0	16.93896	9
AAGACGG	3095	0.0	16.915995	5
CTCTATG	4385	0.0	16.622578	1
CAAGACG	3270	0.0	16.29358	4
CCGCTTA	875	0.0	16.28	25
TCGGTGA	2925	0.0	16.128204	13
CAGTCGG	3025	0.0	15.9008255	10
TCGTTTA	2440	0.0	15.770493	30
TCTATGG	4650	0.0	15.516129	2
CGCTTAT	960	0.0	15.223959	26
ATACACA	1575	0.0	15.152381	37
CGCCTTA	2075	0.0	15.06747	25
CGCAAGA	3405	0.0	15.049927	2
AGACGGA	3420	0.0	15.038012	6
GACGTAT	160	1.0993972E-8	15.03125	1
CGGTGAT	3090	0.0	14.967637	14
TCTTATA	10820	0.0	14.892328	37
>>END_MODULE