FastQCFastQC Report
Fri 10 Feb 2017
ERR1631330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861946
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.2635895984203187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18570.21544273075111434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15050.17460490564374104No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12670.14699296707682383No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC10530.12216542567631847No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10280.11926501196130616No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10210.11845289612110273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.022.0852721
TAGGGGT603.7248945E-521.5833324
TACTGTG957.1395334E-921.4210537
GAATATA450.003825043620.5555551
TACGAGG450.003825043620.55555524
TATACTG450.003825043620.5555555
TCTAGAT1700.019.5882342
ATACACA2750.018.83636537
CTTATAC12600.018.64682637
TAGATAA1900.017.5263164
GCAGTCG4550.017.4835179
CTAGATA1950.017.0769233
GTAAGAT650.001579500317.0769233
CGCCTTA3150.017.03174625
CTCTATG5800.016.9051721
CAGTCGG4750.016.74736810
AGTCGGT4500.016.44444511
TCTATGG6050.016.2066122
CTTATTG4700.015.74467928
TCGGTGA4750.015.57894713
Produced by FastQC (version 0.11.5)