##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	861946
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82668287804572	34.0	31.0	34.0	31.0	34.0
2	32.99611692611834	34.0	33.0	34.0	31.0	34.0
3	33.11010434528381	34.0	33.0	34.0	31.0	34.0
4	36.446532613411975	37.0	37.0	37.0	35.0	37.0
5	36.42282695203644	37.0	37.0	37.0	35.0	37.0
6	36.46400818612767	37.0	37.0	37.0	35.0	37.0
7	36.44719506790449	37.0	37.0	37.0	35.0	37.0
8	36.43659811635532	37.0	37.0	37.0	35.0	37.0
9	38.2922491664211	39.0	39.0	39.0	37.0	39.0
10	38.24935668823801	39.0	39.0	39.0	37.0	39.0
11	38.310869822471474	39.0	39.0	39.0	37.0	39.0
12	38.08264786889202	39.0	39.0	39.0	37.0	39.0
13	38.21298434008627	39.0	39.0	39.0	37.0	39.0
14	39.72558373726428	41.0	40.0	41.0	38.0	41.0
15	39.7295282999167	41.0	40.0	41.0	38.0	41.0
16	39.70826826738566	41.0	40.0	41.0	38.0	41.0
17	39.69206075554617	41.0	40.0	41.0	37.0	41.0
18	39.677623656238325	41.0	40.0	41.0	37.0	41.0
19	39.68569724785544	41.0	40.0	41.0	37.0	41.0
20	39.66094627737701	41.0	40.0	41.0	37.0	41.0
21	39.63049657403132	41.0	40.0	41.0	37.0	41.0
22	39.57829492798853	41.0	40.0	41.0	37.0	41.0
23	39.50320901773429	41.0	39.0	41.0	37.0	41.0
24	39.462996521823875	41.0	39.0	41.0	37.0	41.0
25	39.44110187877199	41.0	39.0	41.0	37.0	41.0
26	39.366584449605895	41.0	39.0	41.0	36.0	41.0
27	39.287005218424355	41.0	39.0	41.0	36.0	41.0
28	39.17908314441972	41.0	39.0	41.0	36.0	41.0
29	39.094572049757176	41.0	39.0	41.0	35.0	41.0
30	39.02677313892053	40.0	39.0	41.0	35.0	41.0
31	38.957438168980424	40.0	39.0	41.0	35.0	41.0
32	38.8739781842482	40.0	38.0	41.0	35.0	41.0
33	38.759056831866495	40.0	38.0	41.0	35.0	41.0
34	38.7055128743564	40.0	38.0	41.0	35.0	41.0
35	38.587100584027304	40.0	38.0	41.0	35.0	41.0
36	38.516875767159426	40.0	38.0	41.0	35.0	41.0
37	38.43903446387593	40.0	38.0	41.0	35.0	41.0
38	38.3439275778297	40.0	38.0	41.0	34.0	41.0
39	38.23868200560128	40.0	37.0	41.0	34.0	41.0
40	38.12317128915269	40.0	37.0	41.0	34.0	41.0
41	38.017356075670634	40.0	37.0	41.0	34.0	41.0
42	37.88596385388412	40.0	37.0	41.0	34.0	41.0
43	36.905088021755425	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	1.0
13	3.0
14	2.0
15	2.0
16	2.0
17	12.0
18	20.0
19	30.0
20	71.0
21	147.0
22	244.0
23	451.0
24	630.0
25	1038.0
26	1413.0
27	2133.0
28	2908.0
29	4066.0
30	5345.0
31	7148.0
32	9261.0
33	12724.0
34	18965.0
35	27825.0
36	45948.0
37	87502.0
38	191741.0
39	442312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71747186018614	17.29273063509779	11.976736361674629	27.013061143041444
2	20.537945532550765	19.130664798026793	32.31698969541015	28.014399974012292
3	20.964422365206172	20.305564385703978	27.95488348458024	30.77512976450961
4	15.2214871929332	15.026695407832975	32.10607161005446	37.64574578917937
5	17.341573602058595	33.804437865017064	31.32655642000775	17.527432112916586
6	36.19437876618721	32.46467876177858	15.272998540511818	16.06794393152239
7	31.857912212598006	28.312678520464157	19.131476913866994	20.69793235307084
8	29.908254113366727	30.043877458680708	18.51090439540296	21.5369640325496
9	28.087606416179206	13.327749070127362	18.307179336060493	40.27746517763294
10	19.74334819118599	23.927368999914147	29.085000684497636	27.244282124402226
11	39.16347427797101	20.271919586609837	18.80048170070979	21.764124434709366
12	24.212305643276956	24.07888661238639	25.498349084513418	26.21045865982324
13	32.613644010181616	17.094458353539547	22.981254046077133	27.310643590201707
14	24.775333953635144	19.105257173883285	22.055790037891004	34.063618834590564
15	28.317667232053978	24.91559796089314	19.795787671153413	26.970947135899465
16	27.988992349868784	23.389864330248066	21.763196302320566	26.857947017562584
17	26.51743844742014	23.896624614535018	22.55872177607414	27.0272151619707
18	26.711882182874565	21.68268081759182	24.18991444939706	27.41552255013655
19	27.188478164525385	23.509709424952373	23.19565262789084	26.106159782631394
20	27.557642822172156	21.989428572091523	23.30621639870711	27.14671220702921
21	29.04277066080706	22.583781350571847	22.572295712260395	25.8011522763607
22	28.999264455081875	21.89870363108594	22.19234151559378	26.909690398238407
23	27.08023472468113	22.05242555798159	23.00144092553362	27.865898791803662
24	26.538669475814032	23.076851682123937	22.99946864420741	27.38501019785462
25	26.96746663944145	21.985367992890506	22.98786698934736	28.059298378320683
26	27.154833365431248	23.11038046466948	22.969768407765685	26.765017762133592
27	27.705099855443382	22.034442992948513	22.70757100792857	27.552886143679533
28	26.36197627229548	22.65768389203036	23.787453042301955	27.192886793372207
29	26.148389806321976	22.878579400565695	24.257900146876953	26.71513064623538
30	25.599747547990248	23.470147781879607	24.208825146818942	26.721279523311203
31	26.808639984407378	22.939952154775355	22.6916767407703	27.559731120046965
32	25.783401744424822	22.555473312713325	23.657630524418003	28.003494418443847
33	25.41493318606966	22.143150498987175	24.43958206198532	28.002334252957844
34	26.962593944400233	21.883853512865077	24.166479106579764	26.987073436154933
35	26.590528873038448	21.927591751687462	24.737628575339986	26.744250799934104
36	24.999593942079898	22.642137674517894	24.64446728681379	27.71380109658842
37	25.604388209934264	21.174992400916068	25.788738505660447	27.431880883489224
38	24.62996521823873	20.519962967517685	26.63264288018043	28.217428934063154
39	24.734612145076373	20.333872423562497	27.466105765326365	27.465409666034763
40	24.249895005023518	20.56300510704847	27.80208969007339	27.38501019785462
41	22.949465511760597	20.79956284964487	29.258097375009573	26.99287426358496
42	23.292874495618054	21.030667814457054	29.045090991779066	26.631366698145825
43	22.096395829901176	20.221916454163022	29.315757599664018	28.365930116271787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	25.0
2	36.0
3	108.5
4	181.0
5	181.0
6	231.5
7	282.0
8	298.5
9	315.0
10	478.0
11	641.0
12	641.0
13	1212.5
14	1784.0
15	3046.0
16	4308.0
17	3879.5
18	3451.0
19	3451.0
20	3890.0
21	4329.0
22	3644.5
23	2960.0
24	3352.0
25	3744.0
26	3744.0
27	4337.0
28	4930.0
29	6180.0
30	7430.0
31	9141.5
32	10853.0
33	10853.0
34	13942.5
35	17032.0
36	20806.5
37	24581.0
38	28040.0
39	31499.0
40	31499.0
41	34581.5
42	37664.0
43	40821.0
44	43978.0
45	47353.5
46	50729.0
47	50729.0
48	55461.0
49	60193.0
50	61923.5
51	63654.0
52	65731.5
53	67809.0
54	67809.0
55	66523.5
56	65238.0
57	63559.0
58	61880.0
59	61791.0
60	61702.0
61	61702.0
62	59044.0
63	56386.0
64	52122.0
65	47858.0
66	43438.0
67	39018.0
68	39018.0
69	34143.5
70	29269.0
71	25898.0
72	22527.0
73	17922.5
74	13318.0
75	13318.0
76	10480.0
77	7642.0
78	6443.5
79	5245.0
80	4513.0
81	3781.0
82	3781.0
83	3048.0
84	2315.0
85	1979.0
86	1643.0
87	1347.0
88	1051.0
89	1051.0
90	778.0
91	505.0
92	307.0
93	109.0
94	78.0
95	47.0
96	47.0
97	29.0
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	861946.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.192146799057866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.12267023333912	52.75362179575834
2	5.936868303104141	7.028319602480278
3	1.6407345474085164	2.91355800565472
4	0.7689453748860122	1.8206211004283768
5	0.4623357427122767	1.3683322576538264
6	0.3234806259877177	1.1488507620069672
7	0.22269451592146738	0.9227236534438037
8	0.18261513631725343	0.8647505565296664
9	0.12604210885071518	0.6714632708958927
>10	0.9823336179311585	11.630432226771575
>50	0.13098105873878266	5.391256817352296
>100	0.09498880178753867	10.906779042010829
>500	0.003933283715064591	1.414844144430644
>1k	0.0013766493002726067	1.1644467645828316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2272	0.2635895984203187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1857	0.21544273075111434	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1505	0.17460490564374104	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1267	0.14699296707682383	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1053	0.12216542567631847	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1028	0.11926501196130616	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1021	0.11845289612110273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1601654860049238E-4	0.0	0.0	0.0
3	0.0	1.1601654860049238E-4	0.0	0.0	0.0
4	0.0	1.1601654860049238E-4	0.0	0.0	0.0
5	0.0	1.1601654860049238E-4	0.0	0.0	0.0
6	0.0	1.1601654860049238E-4	0.0	0.0	0.0
7	0.0	1.1601654860049238E-4	0.0	0.0	0.0
8	0.0	1.1601654860049238E-4	0.0	0.0	0.0
9	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.0	0.0
10	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.0	0.0
11	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.0	0.0
12	0.0	1.1601654860049238E-4	1.1601654860049238E-4	1.1601654860049238E-4	0.0
13	0.0	1.1601654860049238E-4	1.1601654860049238E-4	1.1601654860049238E-4	0.0
14	0.0	1.1601654860049238E-4	1.1601654860049238E-4	1.1601654860049238E-4	0.0
15	0.0	1.1601654860049238E-4	1.1601654860049238E-4	2.3203309720098476E-4	0.0
16	0.0	1.1601654860049238E-4	1.1601654860049238E-4	9.28132388803939E-4	0.0
17	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.0016242316804068932	0.0
18	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.0016242316804068932	0.0
19	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.001856264777607878	0.0
20	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.002204314423409355	0.0
21	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.003132446812213294	0.0
22	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.004292612298218218	0.0
23	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.006612943270228065	0.0
24	0.0	1.1601654860049238E-4	1.1601654860049238E-4	0.009745390082441359	0.0
25	0.0	2.3203309720098476E-4	1.1601654860049238E-4	0.012877836894654654	0.0
26	0.0	2.3203309720098476E-4	1.1601654860049238E-4	0.020070862907885182	0.0
27	0.0	2.3203309720098476E-4	1.1601654860049238E-4	0.05359964545342748	0.0
28	0.0	2.3203309720098476E-4	1.1601654860049238E-4	0.20012854633584934	0.0
29	0.0	2.3203309720098476E-4	1.1601654860049238E-4	0.410234515851341	0.0
30	0.0	2.3203309720098476E-4	1.1601654860049238E-4	0.6641947407378188	0.0
31	0.0	2.3203309720098476E-4	1.1601654860049238E-4	1.4017119401911489	1.1601654860049238E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1290	0.0	22.085272	1
TAGGGGT	60	3.7248945E-5	21.583332	4
TACTGTG	95	7.1395334E-9	21.421053	7
GAATATA	45	0.0038250436	20.555555	1
TACGAGG	45	0.0038250436	20.555555	24
TATACTG	45	0.0038250436	20.555555	5
TCTAGAT	170	0.0	19.588234	2
ATACACA	275	0.0	18.836365	37
CTTATAC	1260	0.0	18.646826	37
TAGATAA	190	0.0	17.526316	4
GCAGTCG	455	0.0	17.483517	9
CTAGATA	195	0.0	17.076923	3
GTAAGAT	65	0.0015795003	17.076923	3
CGCCTTA	315	0.0	17.031746	25
CTCTATG	580	0.0	16.905172	1
CAGTCGG	475	0.0	16.747368	10
AGTCGGT	450	0.0	16.444445	11
TCTATGG	605	0.0	16.206612	2
CTTATTG	470	0.0	15.744679	28
TCGGTGA	475	0.0	15.578947	13
>>END_MODULE