FastQCFastQC Report
Fri 10 Feb 2017
ERR1631329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267351
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28100.22172231686407315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.219039555734757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25970.20491560743629822No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24160.19063384965964442No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC20790.16404295258377513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20330.1604133345852885No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20170.15915085875972795No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA19380.1529173843710227No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16700.13177091429288335No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC15140.11946177499366789No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG12920.10194492291401515No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT12920.10194492291401515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA358.8699016E-426.428572
GTTATAC358.8699016E-426.428573
TATTAGA901.4370016E-1024.6666682
ACCGTAC502.702315E-422.1999998
CTTATAC21000.020.1738137
AAGACGG5400.019.5277795
GCTCTAT951.6764534E-719.4736841
CAAGACG5350.019.3644854
GGTATCA35100.019.0797731
CGCAAGA5400.018.8425922
ATTATAG701.2193229E-418.53
ATACGTC500.00703527218.4999985
AGACGGA6000.018.1916666
CAGTCGG20150.018.17866110
AGTCGGT20050.018.08478711
GCAGTCG20500.017.8682949
GGGCAGT20850.017.7458047
GGGTATC2850.017.5263161
CTCTATG30800.017.4788951
ACGGACC5850.017.3931628
Produced by FastQC (version 0.11.5)