FastQCFastQC Report
Fri 10 Feb 2017
ERR1631328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4699673
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA117640.2503152878934343No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC111120.23644198224004095No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC84580.17996996812331412No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC77850.16564982287065505No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC77310.16450080675825743No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT67090.14275461292732494No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC65280.1389032811431774No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT64200.1366052489183822No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC61180.1301792699194178No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG59210.12598748891678208No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC58810.12513636587056162No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC56470.120157296050172No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC56020.11919978262317399No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT54390.11573145620982567No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA51300.10915653067777267No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG50960.1084330760884853No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC50900.10830540763155225No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC50850.10819901725077467No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG49830.10602865348291253No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT49720.10579459464520193No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG49100.10447535392356022No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG48410.10300716666882995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38500.020.8545461
GTCTAGA11050.019.5882361
TCTAGAC10800.019.1851843
CTAGACA11350.019.0704824
ACTGATC10100.018.8663358
AGTCGGT14150.018.30388811
GCAGTCG14200.018.2394379
CTAGAAC12850.018.1400783
TATACAC17200.017.9622137
TAGAACT15200.017.8914474
GTTCTAG11900.017.2563041
CTTATAC54100.017.02957537
CATATAA2450.016.6122462
CTCTATG23300.016.5944211
AACGGAG10550.016.30805625
ACATGTA8400.016.2976198
CAGTCGG16200.015.98765510
ACGGAGT10650.015.9812226
TCTATGG24900.015.8995982
AAGACGG14250.015.8385965
Produced by FastQC (version 0.11.5)