##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631328.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4699673
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87458872138551	34.0	33.0	34.0	31.0	34.0
2	33.039235070184674	34.0	33.0	34.0	31.0	34.0
3	33.153847937931	34.0	33.0	34.0	31.0	34.0
4	36.47453876046269	37.0	37.0	37.0	35.0	37.0
5	36.45714329486328	37.0	37.0	37.0	35.0	37.0
6	36.500995069231415	37.0	37.0	37.0	35.0	37.0
7	36.486917281266166	37.0	37.0	37.0	35.0	37.0
8	36.46565239751787	37.0	37.0	37.0	35.0	37.0
9	38.32684784664806	39.0	39.0	39.0	37.0	39.0
10	38.28781810989828	39.0	39.0	39.0	37.0	39.0
11	38.34806421638272	39.0	39.0	39.0	37.0	39.0
12	38.1285068131336	39.0	39.0	39.0	37.0	39.0
13	38.251335784425855	39.0	39.0	39.0	37.0	39.0
14	39.786640474773456	41.0	40.0	41.0	38.0	41.0
15	39.79708630791972	41.0	40.0	41.0	38.0	41.0
16	39.792314060999566	41.0	40.0	41.0	38.0	41.0
17	39.77513180172323	41.0	40.0	41.0	38.0	41.0
18	39.76474810055934	41.0	40.0	41.0	38.0	41.0
19	39.76682313854602	41.0	40.0	41.0	38.0	41.0
20	39.750032183090184	41.0	40.0	41.0	38.0	41.0
21	39.71812804848337	41.0	40.0	41.0	38.0	41.0
22	39.671974199055974	41.0	40.0	41.0	37.0	41.0
23	39.610263309809	41.0	40.0	41.0	37.0	41.0
24	39.57496000253634	41.0	40.0	41.0	37.0	41.0
25	39.555463539697335	41.0	39.0	41.0	37.0	41.0
26	39.507894485424835	41.0	39.0	41.0	37.0	41.0
27	39.435481787775444	41.0	39.0	41.0	37.0	41.0
28	39.33236397511061	41.0	39.0	41.0	36.0	41.0
29	39.26926469139449	41.0	39.0	41.0	36.0	41.0
30	39.21730384220349	41.0	39.0	41.0	36.0	41.0
31	39.19104988793901	41.0	39.0	41.0	35.0	41.0
32	39.11094027180189	41.0	39.0	41.0	35.0	41.0
33	39.0160362220946	40.0	39.0	41.0	35.0	41.0
34	38.976153234490994	40.0	39.0	41.0	35.0	41.0
35	38.87811471138524	40.0	39.0	41.0	35.0	41.0
36	38.826704538805146	40.0	38.0	41.0	35.0	41.0
37	38.76721188899738	40.0	38.0	41.0	35.0	41.0
38	38.671867170332916	40.0	38.0	41.0	35.0	41.0
39	38.596167435479025	40.0	38.0	41.0	35.0	41.0
40	38.498280837837015	40.0	38.0	41.0	35.0	41.0
41	38.41332471429395	40.0	38.0	41.0	35.0	41.0
42	38.28978909809257	40.0	37.0	41.0	34.0	41.0
43	37.337525610824414	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	1.0
13	2.0
14	2.0
15	9.0
16	24.0
17	35.0
18	69.0
19	168.0
20	297.0
21	569.0
22	1006.0
23	1825.0
24	2737.0
25	4368.0
26	6291.0
27	9018.0
28	12896.0
29	17999.0
30	24326.0
31	32739.0
32	44007.0
33	60885.0
34	90700.0
35	135316.0
36	225371.0
37	420383.0
38	1011497.0
39	2597130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18490392842225	16.970648808970328	11.550888753323902	27.293558509283518
2	20.388631294134722	19.289661216854874	32.33237716751782	27.989330321492577
3	20.428400018469368	20.103568907879335	28.141468566004484	31.32656250764681
4	14.68040436004803	14.415279531150361	32.69078508228125	38.21353102652035
5	16.85221503708875	34.09845748842526	31.875068754783577	17.17425871970241
6	37.14679723461611	32.89611851718194	14.896674726092646	15.060409522109305
7	32.16040775602899	28.38325134535956	19.375560810294672	20.080780088316782
8	29.22692706492558	30.58644718472966	18.92933827523745	21.25728747510731
9	27.52800035236494	13.612010878203654	18.054192281037427	40.80579648839398
10	18.80547859393622	24.017202899010208	29.81424452297	27.363073984083574
11	39.690272067865145	20.048118241418074	19.246871005706144	21.014738685010638
12	24.953289303319615	23.604195440831734	25.739471661113445	25.70304359473521
13	31.678927448782073	16.81685087451829	24.04809866558801	27.45612301111162
14	24.619904406115065	18.887484299439556	21.6894664799019	34.80314481454348
15	27.557321541307232	25.346997546425037	19.891171151695023	27.204509760572705
16	28.156980283521854	23.5990461464021	21.42221384338868	26.821759726687368
17	26.845676284286164	24.0200754392912	22.05095971570788	27.083288560714756
18	27.495423617770854	21.49336773005271	23.842956733372723	27.168251918803715
19	26.879316922688023	23.12654518729282	22.538568108887574	27.455569781131583
20	27.226213398251325	21.919056921619866	22.899103831266558	27.955625848862255
21	29.4706035930585	22.4467744883527	22.70938850426402	25.373233414324787
22	28.902989633534077	21.848860548382834	21.959676769000737	27.288473049082352
23	26.851697979838175	21.823539637757776	23.151163921404745	28.173598460999305
24	26.439222473563582	23.003919634408607	22.83046075758888	27.72639713443893
25	27.23353305644882	22.105899708341408	22.8577605292964	27.80280670591337
26	27.635369524645654	23.277917421063123	22.54731339818749	26.539399656103736
27	28.140936614100596	21.68808340495179	22.7992670979449	27.371712883002715
28	26.406071230913298	22.306764747249435	23.699095660485312	27.588068361351947
29	25.833712260406205	22.590252555869313	23.68707354745745	27.888961636267034
30	24.852090773124004	23.781760986349475	24.76355269824092	26.602595542285602
31	27.567705242471124	22.976066632721043	22.053789699836564	27.40243842497127
32	26.41098646650522	22.32885139029886	22.83499298781	28.425169155385916
33	25.922441837974684	21.88831010157515	23.761972375524852	28.427275684925313
34	27.406970655192392	21.71251063637832	23.51259332298226	27.367925385447027
35	26.897658624334074	21.73459727942774	24.595966570440115	26.771777525798075
36	25.441727541469373	22.5859543844859	24.570752050195832	27.401566023848893
37	26.8975522339533	21.349868384459942	24.7690637199652	26.983515661621567
38	25.25288461558921	20.566537288870947	26.21946250302947	27.96111559251037
39	26.357195489984093	20.38422673237053	25.873949102416276	27.384628675229106
40	25.301419907299934	21.40453176210345	26.12628580754448	27.167762523052136
41	24.524004116882175	21.12583152061856	27.58625972487873	26.763904637620534
42	25.55950169299013	21.053209446699803	27.319836933335573	26.067451926974496
43	23.09128741510314	20.34971369284629	27.626624235345737	28.93237465670484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	56.0
2	77.0
3	254.0
4	431.0
5	431.0
6	559.5
7	688.0
8	667.0
9	646.0
10	989.0
11	1332.0
12	1332.0
13	2216.0
14	3100.0
15	5028.0
16	6956.0
17	6624.0
18	6292.0
19	6292.0
20	7407.5
21	8523.0
22	7846.0
23	7169.0
24	8684.5
25	10200.0
26	10200.0
27	13209.5
28	16219.0
29	21344.5
30	26470.0
31	33985.0
32	41500.0
33	41500.0
34	57478.5
35	73457.0
36	96953.5
37	120450.0
38	146997.0
39	173544.0
40	173544.0
41	189233.5
42	204923.0
43	225823.0
44	246723.0
45	279626.0
46	312529.0
47	312529.0
48	337995.0
49	363461.0
50	376515.5
51	389570.0
52	404394.5
53	419219.0
54	419219.0
55	404207.5
56	389196.0
57	379321.5
58	369447.0
59	361109.0
60	352771.0
61	352771.0
62	331407.5
63	310044.0
64	291376.5
65	272709.0
66	246554.5
67	220400.0
68	220400.0
69	178702.5
70	137005.0
71	116125.0
72	95245.0
73	69996.5
74	44748.0
75	44748.0
76	34348.5
77	23949.0
78	19811.5
79	15674.0
80	14357.5
81	13041.0
82	13041.0
83	10442.0
84	7843.0
85	7145.0
86	6447.0
87	5466.0
88	4485.0
89	4485.0
90	3468.0
91	2451.0
92	1445.0
93	439.0
94	306.5
95	174.0
96	174.0
97	123.0
98	72.0
99	45.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4699673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.4813691533979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.15395940884896	27.506270423809394
2	8.704192383831495	5.828565567705136
3	2.866217256600008	2.878946341261928
4	1.4479113111667512	1.939122124422175
5	0.8937401346499247	1.496182168771084
6	0.6005631675898337	1.2064606268405516
7	0.44595898172188264	1.0451922106012648
8	0.3356383305077518	0.8990104676608169
9	0.2710564528455819	0.8167807043220168
>10	1.8066905033494036	11.888434020068651
>50	0.20700541648514492	4.877398444855944
>100	0.210660958910534	14.91570642858046
>500	0.032058829185553556	7.411782686109516
>1k	0.023199629863519713	14.69706520956816
>5k	0.0010197639500448225	2.1049216995269586
>10k+	1.274704937556028E-4	0.4881608758959723
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	11764	0.2503152878934343	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	11112	0.23644198224004095	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	8458	0.17996996812331412	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	7785	0.16564982287065505	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	7731	0.16450080675825743	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	6709	0.14275461292732494	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	6528	0.1389032811431774	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6420	0.1366052489183822	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	6118	0.1301792699194178	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	5921	0.12598748891678208	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	5881	0.12513636587056162	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	5647	0.120157296050172	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	5602	0.11919978262317399	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	5439	0.11573145620982567	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	5130	0.10915653067777267	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	5096	0.1084330760884853	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	5090	0.10830540763155225	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	5085	0.10819901725077467	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	4983	0.10602865348291253	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	4972	0.10579459464520193	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	4910	0.10447535392356022	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	4841	0.10300716666882995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.255615231102249E-5	0.0	0.0	0.0
3	0.0	6.383422846653374E-5	0.0	0.0	0.0
4	0.0	6.383422846653374E-5	0.0	0.0	0.0
5	0.0	8.511230462204498E-5	0.0	0.0	0.0
6	0.0	8.511230462204498E-5	0.0	0.0	0.0
7	0.0	8.511230462204498E-5	0.0	0.0	0.0
8	0.0	8.511230462204498E-5	0.0	0.0	0.0
9	0.0	1.0639038077755623E-4	0.0	2.1278076155511244E-5	0.0
10	0.0	1.0639038077755623E-4	0.0	4.255615231102249E-5	2.1278076155511244E-5
11	0.0	1.489465330885787E-4	0.0	4.255615231102249E-5	2.1278076155511244E-5
12	0.0	1.489465330885787E-4	0.0	4.255615231102249E-5	4.255615231102249E-5
13	0.0	1.489465330885787E-4	0.0	6.383422846653374E-5	4.255615231102249E-5
14	0.0	1.489465330885787E-4	0.0	1.0639038077755623E-4	4.255615231102249E-5
15	0.0	1.489465330885787E-4	0.0	1.2766845693306747E-4	4.255615231102249E-5
16	0.0	1.7022460924408995E-4	6.383422846653374E-5	3.830053707992024E-4	4.255615231102249E-5
17	0.0	1.7022460924408995E-4	6.383422846653374E-5	5.745080561988036E-4	4.255615231102249E-5
18	0.0	1.7022460924408995E-4	6.383422846653374E-5	6.808984369763598E-4	4.255615231102249E-5
19	0.0	1.7022460924408995E-4	6.383422846653374E-5	8.936791985314723E-4	4.255615231102249E-5
20	0.0	1.7022460924408995E-4	8.511230462204498E-5	0.0010851818839310735	4.255615231102249E-5
21	0.0	1.7022460924408995E-4	8.511230462204498E-5	0.0014469091785747647	4.255615231102249E-5
22	0.0	1.7022460924408995E-4	8.511230462204498E-5	0.0021490856917066358	4.255615231102249E-5
23	0.0	2.1278076155511246E-4	8.511230462204498E-5	0.003298101804104243	4.255615231102249E-5
24	0.0	2.1278076155511246E-4	8.511230462204498E-5	0.005532299800432924	4.255615231102249E-5
25	0.0	2.1278076155511246E-4	8.511230462204498E-5	0.007958000482161205	4.255615231102249E-5
26	0.0	2.1278076155511246E-4	8.511230462204498E-5	0.012788123769462258	4.255615231102249E-5
27	0.0	2.340588377106237E-4	8.511230462204498E-5	0.03625784176899116	4.255615231102249E-5
28	0.0	2.5533691386613494E-4	8.511230462204498E-5	0.13960545765630927	4.255615231102249E-5
29	0.0	2.978930661771574E-4	8.511230462204498E-5	0.2910202475789273	4.255615231102249E-5
30	0.0	2.978930661771574E-4	8.511230462204498E-5	0.5056309236834137	4.255615231102249E-5
31	0.0	2.978930661771574E-4	8.511230462204498E-5	1.0697552787183278	4.255615231102249E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3850	0.0	20.854546	1
GTCTAGA	1105	0.0	19.588236	1
TCTAGAC	1080	0.0	19.185184	3
CTAGACA	1135	0.0	19.070482	4
ACTGATC	1010	0.0	18.866335	8
AGTCGGT	1415	0.0	18.303888	11
GCAGTCG	1420	0.0	18.239437	9
CTAGAAC	1285	0.0	18.140078	3
TATACAC	1720	0.0	17.96221	37
TAGAACT	1520	0.0	17.891447	4
GTTCTAG	1190	0.0	17.256304	1
CTTATAC	5410	0.0	17.029575	37
CATATAA	245	0.0	16.612246	2
CTCTATG	2330	0.0	16.594421	1
AACGGAG	1055	0.0	16.308056	25
ACATGTA	840	0.0	16.297619	8
CAGTCGG	1620	0.0	15.987655	10
ACGGAGT	1065	0.0	15.98122	26
TCTATGG	2490	0.0	15.899598	2
AAGACGG	1425	0.0	15.838596	5
>>END_MODULE