FastQCFastQC Report
Fri 10 Feb 2017
ERR1631327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4885
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC160.3275332650972364No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT150.3070624360286591RNA PCR Primer, Index 39 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150.3070624360286591No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG110.22517911975435007No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC110.22517911975435007No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA110.22517911975435007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100.2047082906857728No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG100.2047082906857728No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC90.1842374616171955No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC80.1637666325486182No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA80.1637666325486182No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC80.1637666325486182RNA PCR Primer, Index 39 (95% over 21bp)
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT80.1637666325486182No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA80.1637666325486182No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG70.14329580348004092No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT70.14329580348004092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.14329580348004092No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC70.14329580348004092No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG60.12282497441146367No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA60.12282497441146367No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC60.12282497441146367No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC60.12282497441146367No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC60.12282497441146367No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC60.12282497441146367No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC60.12282497441146367No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA60.12282497441146367No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC60.12282497441146367No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCG60.12282497441146367No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT60.12282497441146367No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG50.1023541453428864No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG50.1023541453428864No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA50.1023541453428864No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT50.1023541453428864No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC50.1023541453428864No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC50.1023541453428864No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGT50.1023541453428864No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA50.1023541453428864No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC50.1023541453428864No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT50.1023541453428864No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG50.1023541453428864No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)