##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4885
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42333674513818	34.0	31.0	34.0	31.0	34.0
2	32.6114636642784	34.0	31.0	34.0	31.0	34.0
3	32.70051177072671	34.0	31.0	34.0	31.0	34.0
4	36.1582395087001	37.0	35.0	37.0	35.0	37.0
5	36.13080859774821	37.0	35.0	37.0	35.0	37.0
6	36.1525076765609	37.0	36.0	37.0	35.0	37.0
7	36.20614124872057	37.0	36.0	37.0	35.0	37.0
8	36.21842374616172	37.0	37.0	37.0	35.0	37.0
9	37.969293756397136	39.0	38.0	39.0	35.0	39.0
10	37.954145342886385	39.0	38.0	39.0	35.0	39.0
11	37.989150460593656	39.0	38.0	39.0	35.0	39.0
12	37.79181166837257	39.0	38.0	39.0	35.0	39.0
13	37.95639713408393	39.0	38.0	39.0	35.0	39.0
14	39.30747185261003	40.0	39.0	41.0	36.0	41.0
15	39.31381780962129	40.0	39.0	41.0	36.0	41.0
16	39.275742067553736	40.0	39.0	41.0	36.0	41.0
17	39.2661207778915	40.0	39.0	41.0	36.0	41.0
18	39.280245649948824	40.0	39.0	41.0	36.0	41.0
19	39.22927328556806	40.0	39.0	41.0	36.0	41.0
20	39.2618219037871	40.0	39.0	41.0	36.0	41.0
21	39.240327533265095	41.0	39.0	41.0	36.0	41.0
22	39.115864892528144	40.0	39.0	41.0	36.0	41.0
23	39.01494370522006	40.0	39.0	41.0	35.0	41.0
24	38.93654042988741	40.0	39.0	41.0	35.0	41.0
25	38.99651995905834	40.0	39.0	41.0	35.0	41.0
26	38.97256908904811	40.0	39.0	41.0	35.0	41.0
27	38.85813715455476	40.0	38.0	41.0	35.0	41.0
28	38.699078812691916	40.0	38.0	41.0	35.0	41.0
29	38.671852610030705	40.0	38.0	41.0	35.0	41.0
30	38.57400204708291	40.0	38.0	41.0	35.0	41.0
31	38.52016376663255	40.0	38.0	41.0	34.0	41.0
32	38.37932446264074	40.0	38.0	41.0	34.0	41.0
33	38.27881269191402	40.0	38.0	41.0	34.0	41.0
34	38.2388945752303	40.0	38.0	41.0	34.0	41.0
35	38.22026612077789	40.0	38.0	41.0	34.0	41.0
36	38.14206755373593	40.0	37.0	41.0	33.0	41.0
37	38.08004094165814	40.0	37.0	41.0	33.0	41.0
38	37.982190378710335	40.0	37.0	41.0	33.0	41.0
39	37.901330603889456	40.0	37.0	41.0	33.0	41.0
40	37.68454452405322	40.0	37.0	41.0	33.0	41.0
41	37.61207778915046	40.0	36.0	41.0	33.0	41.0
42	37.401637666325485	40.0	36.0	41.0	33.0	41.0
43	36.36171954964176	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	5.0
24	7.0
25	11.0
26	15.0
27	23.0
28	29.0
29	31.0
30	47.0
31	70.0
32	64.0
33	85.0
34	143.0
35	233.0
36	334.0
37	543.0
38	1093.0
39	2149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97134083930399	16.786079836233366	12.425793244626407	26.816786079836234
2	23.213920163766634	17.830092118730807	32.3439099283521	26.61207778915046
3	20.59365404298874	21.003070624360287	27.533265097236438	30.870010235414536
4	14.943705220061412	15.47594677584442	32.54861821903787	37.03172978505629
5	17.993858751279426	33.75639713408393	30.52200614124872	17.72773797338792
6	34.96417604912999	32.95803480040942	15.47594677584442	16.601842374616172
7	32.11873080859775	27.942681678607983	18.93551688843398	21.003070624360287
8	30.255885363357216	28.863868986693962	17.09314227226203	23.787103377686798
9	28.618219037871036	13.592630501535313	19.140225179119756	38.6489252814739
10	21.12589559877175	22.90685772773797	27.14431934493347	28.82292732855681
11	38.546571136131014	21.228249744114635	17.46161719549642	22.76356192425793
12	25.772773797338793	24.83111566018424	23.561924257932446	25.834186284544526
13	32.97850562947799	17.07267144319345	23.31627430910952	26.632548618219037
14	24.503582395087	20.08188331627431	21.003070624360287	34.4114636642784
15	30.68577277379734	24.36028659160696	18.075742067553733	26.878198567041967
16	29.150460593654042	23.132036847492323	21.473899692937565	26.24360286591607
17	26.9396110542477	24.872057318321392	21.12589559877175	27.06243602865916
18	27.61514841351075	20.184237461617197	23.254861821903788	28.94575230296827
19	26.325486182190378	24.44216990788127	23.561924257932446	25.670419651995907
20	27.922210849539407	20.859774820880244	23.520982599795293	27.697031729785053
21	29.150460593654042	21.37154554759468	24.032753326509724	25.445240532241552
22	31.258955987717503	20.245649948822926	20.777891504605936	27.717502558853635
23	26.18219037871034	20.51177072671443	23.254861821903788	30.051177072671443
24	25.813715455475943	23.3367451381781	23.664278403275333	27.185261003070625
25	26.816786079836234	22.16990788126919	22.374616171954965	28.63868986693961
26	28.065506653019444	22.78403275332651	21.88331627430911	27.267144319344933
27	28.126919140225183	21.985670419651996	22.681678607983624	27.2057318321392
28	25.158648925281472	21.699078812691912	24.728761514841352	28.41351074718526
29	24.544524053224155	20.941658137154555	25.977482088024566	28.53633572159672
30	23.76663254861822	23.82804503582395	26.571136131013308	25.834186284544526
31	27.61514841351075	23.172978505629477	22.10849539406346	27.103377686796314
32	24.749232343909927	22.743091095189357	25.07676560900716	27.43091095189355
33	25.8546571136131	20.67553735926305	26.571136131013308	26.89866939611054
34	26.796315250767655	22.067553735926303	24.176049129989767	26.960081883316274
35	26.89866939611054	23.172978505629477	24.13510747185261	25.79324462640737
36	24.605936540429887	23.418628454452406	24.421699078812694	27.553735926305016
37	26.61207778915046	20.69600818833163	26.141248720573184	26.550665301944733
38	24.073694984646878	19.733879222108495	27.164790174002047	29.02763561924258
39	25.465711361310134	19.672466734902763	27.021494370522003	27.840327533265096
40	23.500511770726714	19.83623336745138	27.922210849539407	28.741044012282497
41	22.886386898669397	21.269191402251792	28.761514841351076	27.08290685772774
42	22.231320368474925	20.573183213920164	30.010235414534286	27.185261003070625
43	20.061412487205732	20.55271238485159	30.092118730808597	29.293756397134086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	2.5
7	4.0
8	3.0
9	2.0
10	3.5
11	5.0
12	5.0
13	7.0
14	9.0
15	12.0
16	15.0
17	19.5
18	24.0
19	24.0
20	30.0
21	36.0
22	21.5
23	7.0
24	10.0
25	13.0
26	13.0
27	13.0
28	13.0
29	21.5
30	30.0
31	36.0
32	42.0
33	42.0
34	50.5
35	59.0
36	72.0
37	85.0
38	112.5
39	140.0
40	140.0
41	160.0
42	180.0
43	208.5
44	237.0
45	274.5
46	312.0
47	312.0
48	381.5
49	451.0
50	446.0
51	441.0
52	442.5
53	444.0
54	444.0
55	414.0
56	384.0
57	375.5
58	367.0
59	357.5
60	348.0
61	348.0
62	317.0
63	286.0
64	290.0
65	294.0
66	269.5
67	245.0
68	245.0
69	205.5
70	166.0
71	134.0
72	102.0
73	81.5
74	61.0
75	61.0
76	46.0
77	31.0
78	27.0
79	23.0
80	16.0
81	9.0
82	9.0
83	8.0
84	7.0
85	6.5
86	6.0
87	3.5
88	1.0
89	1.0
90	2.0
91	3.0
92	1.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4885.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.07676560900717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.85659287776708	77.2978505629478
2	5.8469682386910495	9.948822927328557
3	1.4918190567853706	3.8075742067553735
4	0.8421559191530318	2.865916069600819
5	0.2646775745909528	1.1258955987717503
6	0.2646775745909528	1.3510747185261003
7	0.0962463907603465	0.5731832139201637
8	0.1203079884504331	0.8188331627430911
9	0.024061597690086624	0.1842374616171955
>10	0.192492781520693	2.0266120777891503
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	16	0.3275332650972364	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	15	0.3070624360286591	RNA PCR Primer, Index 39 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.3070624360286591	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	11	0.22517911975435007	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	11	0.22517911975435007	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	11	0.22517911975435007	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.2047082906857728	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	10	0.2047082906857728	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	9	0.1842374616171955	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	8	0.1637666325486182	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	8	0.1637666325486182	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	8	0.1637666325486182	RNA PCR Primer, Index 39 (95% over 21bp)
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	8	0.1637666325486182	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	8	0.1637666325486182	No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG	7	0.14329580348004092	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	7	0.14329580348004092	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.14329580348004092	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	7	0.14329580348004092	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	6	0.12282497441146367	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.12282497441146367	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	6	0.12282497441146367	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	6	0.12282497441146367	No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC	6	0.12282497441146367	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	6	0.12282497441146367	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	6	0.12282497441146367	No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA	6	0.12282497441146367	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	6	0.12282497441146367	No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCG	6	0.12282497441146367	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	6	0.12282497441146367	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	5	0.1023541453428864	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	5	0.1023541453428864	No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA	5	0.1023541453428864	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	5	0.1023541453428864	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	5	0.1023541453428864	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	5	0.1023541453428864	No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGT	5	0.1023541453428864	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	5	0.1023541453428864	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	5	0.1023541453428864	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	5	0.1023541453428864	No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG	5	0.1023541453428864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.02047082906857728	0.0
24	0.0	0.0	0.0	0.02047082906857728	0.0
25	0.0	0.0	0.0	0.02047082906857728	0.0
26	0.0	0.0	0.0	0.04094165813715456	0.0
27	0.0	0.0	0.0	0.04094165813715456	0.0
28	0.0	0.0	0.0	0.22517911975435004	0.0
29	0.0	0.0	0.0	0.42988741044012285	0.0
30	0.0	0.0	0.0	0.6960081883316275	0.0
31	0.0	0.0	0.0	1.473899692937564	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE