FastQCFastQC Report
Fri 10 Feb 2017
ERR1631326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428722
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52260.36578144663552464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46100.3226659910045481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38530.2696815755619358No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA19200.13438583573291374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.12290704559739403No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16360.11450793086408692No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC15690.10981842513799046No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC15000.10498893416633887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.021.0543941
GCAGTCG4300.018.5000029
AGTCGGT4300.018.50000211
CAGTCGG4350.018.28735510
TCGGTGA4750.017.13684313
CGGTGAT4650.016.70967714
TATACAC8450.016.20118337
GTCGGTG4950.016.07070712
TATTCAG1403.4746336E-815.8571425
GGACGTA700.002592907815.85714227
GTATAGA1059.346197E-615.8571421
TTAGAAC957.061593E-515.5789483
GGCAGTC5300.015.3584918
CCGCTTA1701.4861143E-915.23529425
CTTATAC20650.015.23002437
TCGTTTA3950.014.98734230
CTTATTG5450.014.9357828
ATACACA5850.014.86324837
CTCTATG8600.014.8430241
CGCTTAT1752.239176E-914.826
Produced by FastQC (version 0.11.5)