FastQCFastQC Report
Fri 10 Feb 2017
ERR1631325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79057
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1830.23147855344877746No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1620.20491544075793414No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1310.16570322678573687No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC960.12143137230099801No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC960.12143137230099801No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA940.11890155204472722No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC930.11763664191659182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT930.11763664191659182No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG820.10372263050710247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA851.8189894E-1228.2941191
TATACAC405.8715028E-527.75000236
GCAGTCG905.456968E-1226.7222239
CGGTGAT905.456968E-1226.72222314
TCGGTGA905.456968E-1226.72222313
GGGCAGT959.094947E-1225.315797
CAGTCGG959.094947E-1225.3157910
GTCGGTG959.094947E-1225.3157912
ATACACA451.3080478E-424.66666837
GTGATTC1002.0008883E-1124.0516
CGCCTTA1002.0008883E-1124.0525
AGTCGGT1002.0008883E-1124.0511
GCCTTAT952.6921043E-1023.36842226
TGGAGAG400.001915408423.1250025
CTTATTG1053.8198777E-1122.9047628
TCGCCTT1107.094059E-1121.86363624
TCCTCGC1107.094059E-1121.86363621
GGTGATT1107.094059E-1121.86363615
CCTCGCC1059.422365E-1021.14285722
TTCCTCG1059.422365E-1021.14285720