##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79057
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81767585413056	34.0	31.0	34.0	31.0	34.0
2	32.982835169561206	34.0	33.0	34.0	31.0	34.0
3	33.08644395815677	34.0	33.0	34.0	31.0	34.0
4	36.43655843252337	37.0	37.0	37.0	35.0	37.0
5	36.41454899629381	37.0	37.0	37.0	35.0	37.0
6	36.46832032584085	37.0	37.0	37.0	35.0	37.0
7	36.4530908078981	37.0	37.0	37.0	35.0	37.0
8	36.43640664330799	37.0	37.0	37.0	35.0	37.0
9	38.28846275472128	39.0	39.0	39.0	37.0	39.0
10	38.244962495414704	39.0	39.0	39.0	37.0	39.0
11	38.30861277306247	39.0	39.0	39.0	37.0	39.0
12	38.094602628483244	39.0	39.0	39.0	37.0	39.0
13	38.21039250161276	39.0	39.0	39.0	37.0	39.0
14	39.73535550299151	41.0	40.0	41.0	38.0	41.0
15	39.74051633631431	41.0	40.0	41.0	38.0	41.0
16	39.715483764878506	41.0	40.0	41.0	38.0	41.0
17	39.682583452445705	41.0	40.0	41.0	37.0	41.0
18	39.67283099535778	41.0	40.0	41.0	37.0	41.0
19	39.70684442870334	41.0	40.0	41.0	37.0	41.0
20	39.69687693689363	41.0	40.0	41.0	37.0	41.0
21	39.657462337300935	41.0	40.0	41.0	37.0	41.0
22	39.61458188395714	41.0	40.0	41.0	37.0	41.0
23	39.55642131626548	41.0	39.0	41.0	37.0	41.0
24	39.528201171306776	41.0	39.0	41.0	37.0	41.0
25	39.51933415130855	41.0	39.0	41.0	37.0	41.0
26	39.45150967023793	41.0	39.0	41.0	37.0	41.0
27	39.38199020959561	41.0	39.0	41.0	36.0	41.0
28	39.296925003478506	41.0	39.0	41.0	36.0	41.0
29	39.225495528542695	41.0	39.0	41.0	36.0	41.0
30	39.154774403278644	41.0	39.0	41.0	35.0	41.0
31	39.11806671136016	41.0	39.0	41.0	35.0	41.0
32	39.06014647659284	40.0	39.0	41.0	35.0	41.0
33	38.970654085027256	40.0	39.0	41.0	35.0	41.0
34	38.93370606018443	40.0	39.0	41.0	35.0	41.0
35	38.82642903221726	40.0	38.0	41.0	35.0	41.0
36	38.771329547035684	40.0	38.0	41.0	35.0	41.0
37	38.708956828617325	40.0	38.0	41.0	35.0	41.0
38	38.62284174709387	40.0	38.0	41.0	35.0	41.0
39	38.541065307309914	40.0	38.0	41.0	35.0	41.0
40	38.46250173925143	40.0	38.0	41.0	35.0	41.0
41	38.36884779336428	40.0	38.0	41.0	34.0	41.0
42	38.26012876785104	40.0	37.0	41.0	34.0	41.0
43	37.31694853080688	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	5.0
18	3.0
19	2.0
20	10.0
21	16.0
22	25.0
23	44.0
24	46.0
25	91.0
26	107.0
27	149.0
28	240.0
29	362.0
30	429.0
31	579.0
32	774.0
33	1062.0
34	1683.0
35	2364.0
36	3733.0
37	7094.0
38	17408.0
39	42830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.173191494744295	16.05044461591004	12.282277344194695	27.49408654515097
2	20.78626813564896	19.000215034721784	32.08065066976991	28.132866159859343
3	20.198084926066002	20.39161617567072	28.265681723313556	31.14461717494972
4	14.769090656108883	15.330710753001	32.57523052987085	37.32496806101926
5	16.550084116523518	34.35748890041362	32.60052873243356	16.49189825062929
6	36.154926192494024	33.1773277508633	15.939132524634125	14.72861353200855
7	32.511985023464085	28.02914352935224	19.11026221586956	20.348609231314114
8	30.33127996255866	30.49951300960067	18.520814096158468	20.648392931682206
9	28.54902159201589	13.668618844631089	17.446905397371516	40.3354541659815
10	18.963532641005855	23.708210531641726	29.768394955538408	27.559861871814007
11	38.69360081966176	21.129058780373654	18.856015280114345	21.321325119850236
12	25.19953957271336	23.74615783548579	25.81681571524343	25.23748687655742
13	32.366520358728515	16.813185423175682	24.078829199185396	26.741465018910404
14	24.8150068937602	18.686517322944205	21.826024260976258	34.67245152231934
15	28.290979925876265	24.558230137748712	19.848969730700635	27.30182020567439
16	28.36054998292371	23.477996888321083	21.413663559204117	26.747789569551085
17	26.86669112159581	23.937159264834236	22.3421075932555	26.854042020314456
18	27.308144756315063	21.600870258168158	23.878973398940005	27.21201158657677
19	27.27905182336795	23.374274257813983	22.80379979002492	26.542874128793148
20	27.819168448081765	21.536359841633253	23.049192354883186	27.595279355401797
21	29.77598441630722	22.20170256903247	22.103039579037908	25.919273435622397
22	29.373742995560164	21.5667176847085	21.504737088429867	27.554802231301466
23	27.25375362080524	21.83361372174507	22.50401608965683	28.408616567792862
24	26.36705162098233	22.71525608105544	23.17694827782486	27.74074402013737
25	26.836333278520563	21.699533248162716	22.792415598871703	28.67171787444502
26	27.754658031546857	23.189597379106214	22.419267111071758	26.636477478275168
27	28.25176771190407	21.63502283162781	22.26621298556738	27.84699647090074
28	27.018480336972058	22.066357185321984	22.998595949757767	27.916566527948188
29	25.670086140379727	22.453419684531415	24.266035898149436	27.610458276939426
30	25.284288551298427	23.464082876911597	24.554435407364306	26.69719316442567
31	26.966619021718508	23.227544682950278	22.331988312230415	27.473847983100804
32	25.566363509872623	22.11948341070367	24.057325727007097	28.256827352416607
33	25.7763385911431	21.508531818814273	24.50130918198262	28.21382040806001
34	27.01468560658765	21.374451345231922	24.11677650302946	27.49408654515097
35	26.411323475467068	21.330179490747184	24.971855749648988	27.286641284136763
36	24.875722579910697	22.178934186726032	25.315911304501814	27.629431928861454
37	26.079917021895593	20.650922751938474	25.65237739858583	27.6167828275801
38	24.76567539876292	19.559305311357626	27.360006071568616	28.315013218310842
39	24.96300137875204	19.484675613797638	27.771101863212618	27.7812211442377
40	23.753747296254602	19.760426021731156	27.98740149512377	28.49842518689047
41	22.101774668909773	20.20693929696295	30.238941523204776	27.4523445109225
42	23.154179895518425	19.8477048205725	29.77598441630722	27.222130867601855
43	21.058223813198072	19.688326144427435	30.558963785623032	28.694486256751457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	2.0
2	1.0
3	2.5
4	4.0
5	4.0
6	10.0
7	16.0
8	20.0
9	24.0
10	26.5
11	29.0
12	29.0
13	49.0
14	69.0
15	120.0
16	171.0
17	158.0
18	145.0
19	145.0
20	163.5
21	182.0
22	174.5
23	167.0
24	201.0
25	235.0
26	235.0
27	286.5
28	338.0
29	423.0
30	508.0
31	680.0
32	852.0
33	852.0
34	1091.0
35	1330.0
36	1631.5
37	1933.0
38	2340.0
39	2747.0
40	2747.0
41	3021.5
42	3296.0
43	3719.5
44	4143.0
45	4559.0
46	4975.0
47	4975.0
48	5570.0
49	6165.0
50	6430.5
51	6696.0
52	7037.0
53	7378.0
54	7378.0
55	6964.0
56	6550.0
57	6358.0
58	6166.0
59	5927.0
60	5688.0
61	5688.0
62	5451.5
63	5215.0
64	4749.5
65	4284.0
66	3854.5
67	3425.0
68	3425.0
69	2847.5
70	2270.0
71	1988.0
72	1706.0
73	1311.0
74	916.0
75	916.0
76	701.5
77	487.0
78	432.0
79	377.0
80	309.0
81	241.0
82	241.0
83	196.5
84	152.0
85	117.5
86	83.0
87	67.5
88	52.0
89	52.0
90	42.0
91	32.0
92	18.0
93	4.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	79057.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.85321982873117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.74905378047707	65.20611710537966
2	4.478479007129654	6.435862731952895
3	1.512190828272159	3.2596734002049157
4	0.7781005193204823	2.23636110654338
5	0.5017163982043834	1.8024969325929392
6	0.3432796408766834	1.4799448499184134
7	0.2499779948948156	1.2573206673665838
8	0.18836370037848782	1.0827630696838988
9	0.16723879940146114	1.0814981595557636
>10	0.9717454449432268	12.948884981722047
>50	0.0545726608573189	2.606979774087051
>100	0.0052812252442566675	0.6020972209924486
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	183	0.23147855344877746	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	162	0.20491544075793414	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	131	0.16570322678573687	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	96	0.12143137230099801	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	96	0.12143137230099801	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	94	0.11890155204472722	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	93	0.11763664191659182	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	93	0.11763664191659182	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	82	0.10372263050710247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.001264910128135396	0.0	0.0	0.0
17	0.0	0.001264910128135396	0.0	0.003794730384406188	0.0
18	0.0	0.001264910128135396	0.0	0.003794730384406188	0.0
19	0.0	0.001264910128135396	0.0	0.003794730384406188	0.0
20	0.0	0.001264910128135396	0.0	0.003794730384406188	0.0
21	0.0	0.001264910128135396	0.0	0.003794730384406188	0.0
22	0.0	0.001264910128135396	0.0	0.003794730384406188	0.0
23	0.0	0.001264910128135396	0.0	0.005059640512541584	0.0
24	0.0	0.001264910128135396	0.0	0.010119281025083167	0.0
25	0.0	0.001264910128135396	0.0	0.01897365192203094	0.0
26	0.0	0.001264910128135396	0.0	0.026563112690843316	0.0
27	0.0	0.001264910128135396	0.0	0.06957005704744677	0.0
28	0.0	0.001264910128135396	0.0	0.33520118395587994	0.0
29	0.0	0.001264910128135396	0.0	0.693170750218197	0.0
30	0.0	0.001264910128135396	0.0	1.19786989134422	0.0
31	0.0	0.001264910128135396	0.0	2.3881503219196274	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	85	1.8189894E-12	28.294119	1
TATACAC	40	5.8715028E-5	27.750002	36
GCAGTCG	90	5.456968E-12	26.722223	9
CGGTGAT	90	5.456968E-12	26.722223	14
TCGGTGA	90	5.456968E-12	26.722223	13
GGGCAGT	95	9.094947E-12	25.31579	7
CAGTCGG	95	9.094947E-12	25.31579	10
GTCGGTG	95	9.094947E-12	25.31579	12
ATACACA	45	1.3080478E-4	24.666668	37
GTGATTC	100	2.0008883E-11	24.05	16
CGCCTTA	100	2.0008883E-11	24.05	25
AGTCGGT	100	2.0008883E-11	24.05	11
GCCTTAT	95	2.6921043E-10	23.368422	26
TGGAGAG	40	0.0019154084	23.125002	5
CTTATTG	105	3.8198777E-11	22.90476	28
TCGCCTT	110	7.094059E-11	21.863636	24
TCCTCGC	110	7.094059E-11	21.863636	21
GGTGATT	110	7.094059E-11	21.863636	15
CCTCGCC	105	9.422365E-10	21.142857	22
TTCCTCG	105	9.422365E-10	21.142857	20
>>END_MODULE