FastQCFastQC Report
Fri 10 Feb 2017
ERR1631324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424961
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112752.6531846451792047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88432.080896835238998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79011.859229435171698No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.7934845785848583No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA17410.40968465341525456No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA10710.2520231268281089No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA10370.24402239264308961No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10040.23625697416939437No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.22660903000510635No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.21672577012949423No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT8110.19084104188384346No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT7840.18448751767809282No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC7830.1842522019667687No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7810.18378157054412053No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT7810.18378157054412053No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA7760.18260499198750002No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC7670.18048715058558315No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.1750748892251289No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT7330.1724864164005638No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA7060.16613289219481317No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.14236600535107927No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.13083553549619847No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA5410.12730579982633702No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA5370.1263645369810406No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.11506938283748391No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT4840.11389280428086343No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA4830.1136574885695393No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT4620.10871585863173328No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.10448017582789951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC250.005492492629.63
TATTGAC1300.025.61538530
TTTTGGA451.3212957E-424.6666661
ATTGACT1450.024.2413831
CGTTATA400.001929195523.1252
TATTGCT502.6982196E-422.230
GAATTAT705.089445E-621.1428591
GGTACAT450.003821900320.5555551
GGGTCAA450.003821900320.5555557
ACATTCC450.003821900320.5555558
CCCAGAC450.003821900320.55555529
ATATGCT450.003821900320.55555535
TATACAT450.003821900320.5555555
TTATTGC555.135766E-420.18181829
ATACACT851.2415312E-619.5882364
TTGACTG1900.019.47368432
ATACACA2350.018.89361637
GATACAC801.6127662E-518.53
GATACAA500.007027763418.51
ACCGCTG701.21690384E-418.532
Produced by FastQC (version 0.11.5)