FastQCFastQC Report
Fri 10 Feb 2017
ERR1631323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42843
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8501.983988049389632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8351.9489764955768738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6571.5335060569988097No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3910.9126345027192306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.6442125901547511No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2170.5065004784912355No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1790.41780454216558127No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1770.4131363349905469No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1520.3547837453026165No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1450.33844502018999606No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1420.33144270942744436No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1340.3127698807273067No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1150.2684219125644796No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1070.2497490838643419No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1060.2474149802768247No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA990.23107625516420421No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.2170716336391009No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT910.21240342646406649No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA870.2030670121139976No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG860.20073290852648037No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC840.196064701351446No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.19139649417641155No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG750.17505776906379106No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC720.1680554583012394No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.15871904395117056No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT650.15171673318861892No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA650.15171673318861892No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT640.14938262960110169No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.14471442242606727No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT590.13771211166351563No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA560.13070980090096398No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC560.13070980090096398No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA560.13070980090096398No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT550.12837569731344678No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT540.12604159372592955No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT530.12370749013841235No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT520.12137338655089512No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC520.12137338655089512ABI Solid3 Adapter B (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT510.11903928296337792No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT510.11903928296337792No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA490.11437107578834349ABI Solid3 Adapter B (100% over 21bp)
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT490.11437107578834349No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA490.11437107578834349No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.11203697220082628No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT470.10970286861330905No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGT470.10970286861330905No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT460.10736876502579185No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC450.10503466143827463No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC450.10503466143827463No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG440.10270055785075742No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT440.10270055785075742No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.10270055785075742No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGCGGAGGAATC430.10036645426324019No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT430.10036645426324019No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT430.10036645426324019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAGAG250.00543815329.59999822
ATGCCCC508.827126E-625.89999837
TTTTTGC400.00190113823.1250021
ACCGACC400.00190113823.12500232
CGCTTAT1053.6379788E-1122.90476426
TCCGCTT1053.6379788E-1122.90476424
CTCCGCT1053.6379788E-1122.90476423
CCGCTTA1106.730261E-1121.86363625
CCTCCGC1106.730261E-1121.86363622
GCTTATT1106.730261E-1121.86363627
TCCTCCG1059.058567E-1021.14285921
TTCCTCC1151.2369128E-1020.91304420
AGGGGAA450.00376684420.55555510
AGAGGGG450.00376684420.5555558
AGTGGCT450.00376684420.55555527
TGGGAAC555.032791E-420.18181820
GGTATCA6000.020.0416661
TGGGCAG3100.019.0967736
GGCAGTC3150.018.793658
GCAGTCG3100.018.59
Produced by FastQC (version 0.11.5)