##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42843
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75606283406857	34.0	31.0	34.0	31.0	34.0
2	32.8704572508928	34.0	33.0	34.0	31.0	34.0
3	32.85876339191933	34.0	33.0	34.0	31.0	34.0
4	36.31223303690218	37.0	37.0	37.0	35.0	37.0
5	36.314240365987445	37.0	37.0	37.0	35.0	37.0
6	36.41871484256471	37.0	37.0	37.0	35.0	37.0
7	36.390518871227506	37.0	37.0	37.0	35.0	37.0
8	36.41136241626403	37.0	37.0	37.0	35.0	37.0
9	38.211936605746565	39.0	39.0	39.0	37.0	39.0
10	38.20346380972388	39.0	39.0	39.0	37.0	39.0
11	38.26249328945219	39.0	39.0	39.0	37.0	39.0
12	38.066101813598486	39.0	39.0	39.0	37.0	39.0
13	38.16793875312187	39.0	39.0	39.0	37.0	39.0
14	39.56114184347501	41.0	39.0	41.0	37.0	41.0
15	39.55028826179306	41.0	39.0	41.0	37.0	41.0
16	39.39999533179282	41.0	39.0	41.0	37.0	41.0
17	39.38893168078799	40.0	39.0	41.0	37.0	41.0
18	39.57934785145765	41.0	39.0	41.0	37.0	41.0
19	39.622528767826715	41.0	40.0	41.0	37.0	41.0
20	39.66503279415541	41.0	40.0	41.0	37.0	41.0
21	39.66624652802091	41.0	40.0	41.0	37.0	41.0
22	39.58758723712158	41.0	40.0	41.0	37.0	41.0
23	39.49639380995728	41.0	39.0	41.0	36.0	41.0
24	39.44205587843989	41.0	39.0	41.0	36.0	41.0
25	39.39413673178816	41.0	39.0	41.0	36.0	41.0
26	39.26739490698597	41.0	39.0	41.0	35.0	41.0
27	39.11721868216512	41.0	39.0	41.0	35.0	41.0
28	39.00296431155615	41.0	39.0	41.0	35.0	41.0
29	38.83672945405317	40.0	38.0	41.0	35.0	41.0
30	38.628037252293254	40.0	38.0	41.0	35.0	41.0
31	38.40316971267185	40.0	37.0	41.0	35.0	41.0
32	38.1937539387998	40.0	37.0	41.0	35.0	41.0
33	37.99521508764559	40.0	36.0	41.0	35.0	41.0
34	37.8343486683939	40.0	35.0	41.0	34.0	41.0
35	37.642742104894616	40.0	35.0	41.0	34.0	41.0
36	37.48126881871018	40.0	35.0	41.0	33.0	41.0
37	37.282753308591836	40.0	35.0	41.0	33.0	41.0
38	37.06871600961651	40.0	35.0	41.0	33.0	41.0
39	36.81002730901197	39.0	35.0	41.0	33.0	41.0
40	36.58452956142194	39.0	35.0	41.0	32.0	41.0
41	36.26331022570782	39.0	35.0	41.0	31.0	41.0
42	35.97752258245221	39.0	35.0	41.0	30.0	41.0
43	34.96344793781948	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	0.0
17	2.0
18	1.0
19	3.0
20	6.0
21	7.0
22	17.0
23	23.0
24	50.0
25	76.0
26	113.0
27	162.0
28	255.0
29	374.0
30	442.0
31	570.0
32	632.0
33	892.0
34	1197.0
35	1700.0
36	2734.0
37	6588.0
38	9328.0
39	17667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.26993907989637	14.151670051116868	15.839226944891816	24.73916392409495
2	20.922904558504307	19.165324557103844	34.57741054548001	25.334360338911843
3	20.316037625749832	22.078285834325328	30.63277548257592	26.972901057348924
4	13.197021683822326	18.30637443689751	31.573419228345358	36.92318465093481
5	14.303386784305488	38.47069532945872	29.941880820670818	17.28403706556497
6	29.31400695562869	28.893868309875593	21.728170296197746	20.06395443829797
7	30.728473729664124	32.0542445673739	16.926919216674836	20.29036248628714
8	35.79114441098896	27.367364563639335	16.651494993347804	20.189996032023902
9	31.664449268258526	12.001960647013513	16.957262563312558	39.3763275214154
10	22.33503722895222	25.31101930303667	22.103960973788016	30.249982494223094
11	33.20962584319492	25.766169502602526	14.718857222883553	26.305347431319003
12	25.093947669397572	29.58943117895572	23.93623228998903	21.380388861657682
13	35.87984034731461	17.648157225217656	24.652802091356815	21.819200336110917
14	24.69014774875709	21.081623602455476	24.71582288821978	29.512405760567656
15	34.528394370142145	21.79119109306071	20.159652685386177	23.520761851410967
16	25.23866209182363	23.1729804168709	27.035921854211892	24.552435637093577
17	19.660154517657492	29.51707396774269	25.922554442966177	24.90021707163364
18	24.302686553229233	19.99626543425997	28.942884485213455	26.75816352729734
19	23.26634456037159	28.375697313446768	29.360689027379035	18.997269098802605
20	26.30067922414397	20.20633475713652	32.13360408934949	21.359381929370024
21	30.252316597810612	18.703172046775435	29.37936185607917	21.66514949933478
22	28.77482902691221	19.524776509581496	25.27600774922391	26.424386714282377
23	24.475410218705505	22.8065261536307	26.42905492145741	26.289008706206378
24	22.92323133300656	23.14963938099573	31.25131293326798	22.675816352729733
25	22.391055715052634	22.78785332493056	27.241322969913405	27.5797679901034
26	26.942557710711203	20.782858343253274	27.26232990220106	25.012254043834464
27	24.213990616903576	21.18665826389375	26.183974044768103	28.415377074434563
28	21.954578344186913	22.1016268702005	29.937212613495788	26.0065821721168
29	22.41906495810284	22.647807109679526	32.021567117148656	22.911560815068974
30	21.44574376210816	23.70282193123731	33.891184090749945	20.960250215904583
31	22.423733165277877	25.532759143850804	28.420045281609596	23.623462409261723
32	20.295030693462177	24.93289452185888	33.36134257638354	21.410732208295403
33	22.052610694862636	22.230002567513946	33.58775062437271	22.129636113250704
34	23.805522489088066	23.950236911514132	27.27166631655113	24.972574282846676
35	21.50643045538361	26.352029503069346	28.347688070396565	23.793851971150477
36	18.619144317624816	25.058936115584814	30.936208948953155	25.38571061783722
37	20.26001913964942	25.644796116051634	29.839180262820065	24.25600448147889
38	20.752514996615552	21.209999299768924	32.95754265574306	25.079943047872465
39	22.307027985902014	20.941577387204443	32.72413229699134	24.027262329902204
40	20.649814438764793	20.353383283150105	30.72147141890157	28.27533085918353
41	16.651494993347804	22.468081133440702	34.299652218565456	26.580771654646036
42	17.594472842704757	21.40839810470789	33.253973811357746	27.743155241229605
43	16.184674275844362	24.50575356534323	34.74313190019373	24.566440258618677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	4.0
2	7.0
3	37.0
4	67.0
5	67.0
6	94.0
7	121.0
8	115.5
9	110.0
10	192.0
11	274.0
12	274.0
13	505.0
14	736.0
15	1126.0
16	1516.0
17	1376.5
18	1237.0
19	1237.0
20	1381.5
21	1526.0
22	1014.0
23	502.0
24	434.0
25	366.0
26	366.0
27	364.5
28	363.0
29	379.0
30	395.0
31	366.0
32	337.0
33	337.0
34	337.5
35	338.0
36	371.0
37	404.0
38	499.5
39	595.0
40	595.0
41	753.5
42	912.0
43	1279.5
44	1647.0
45	2247.5
46	2848.0
47	2848.0
48	4224.0
49	5600.0
50	5263.0
51	4926.0
52	3797.5
53	2669.0
54	2669.0
55	2239.5
56	1810.0
57	1778.5
58	1747.0
59	1811.0
60	1875.0
61	1875.0
62	1917.0
63	1959.0
64	1938.5
65	1918.0
66	1794.0
67	1670.0
68	1670.0
69	1581.0
70	1492.0
71	1317.5
72	1143.0
73	952.5
74	762.0
75	762.0
76	624.0
77	486.0
78	369.5
79	253.0
80	197.5
81	142.0
82	142.0
83	102.0
84	62.0
85	39.5
86	17.0
87	13.0
88	9.0
89	9.0
90	5.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	42843.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.43559041150246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.7577205494853	60.95978339518708
2	4.258159716122686	5.657867096141727
3	1.3245265783648947	2.639871157481969
4	0.6605066226328918	1.7552458978129448
5	0.4251133049924464	1.4121326704479145
6	0.2670133155324456	1.0643512359078495
7	0.20728665284755646	0.9639847816446093
8	0.1194533253697783	0.6348761758046823
9	0.11593999227066719	0.6932287654926126
>10	0.7237466184168921	9.814905585509885
>50	0.08783332747777817	3.9399668557290575
>100	0.04215999718933352	4.997315780874356
>500	0.01053999929733338	5.466470601965315
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	850	1.983988049389632	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	835	1.9489764955768738	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	657	1.5335060569988097	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	391	0.9126345027192306	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	276	0.6442125901547511	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	217	0.5065004784912355	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	179	0.41780454216558127	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	177	0.4131363349905469	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	152	0.3547837453026165	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	145	0.33844502018999606	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	142	0.33144270942744436	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	134	0.3127698807273067	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	115	0.2684219125644796	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	107	0.2497490838643419	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	106	0.2474149802768247	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	99	0.23107625516420421	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93	0.2170716336391009	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	91	0.21240342646406649	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	87	0.2030670121139976	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	86	0.20073290852648037	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	84	0.196064701351446	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.19139649417641155	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	75	0.17505776906379106	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	72	0.1680554583012394	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68	0.15871904395117056	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	65	0.15171673318861892	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	65	0.15171673318861892	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	64	0.14938262960110169	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62	0.14471442242606727	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	59	0.13771211166351563	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.13070980090096398	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	56	0.13070980090096398	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	56	0.13070980090096398	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	55	0.12837569731344678	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	54	0.12604159372592955	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	53	0.12370749013841235	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	52	0.12137338655089512	No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC	52	0.12137338655089512	ABI Solid3 Adapter B (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	51	0.11903928296337792	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	51	0.11903928296337792	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	49	0.11437107578834349	ABI Solid3 Adapter B (100% over 21bp)
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	49	0.11437107578834349	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	49	0.11437107578834349	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48	0.11203697220082628	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	47	0.10970286861330905	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGT	47	0.10970286861330905	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	46	0.10736876502579185	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC	45	0.10503466143827463	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	45	0.10503466143827463	No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG	44	0.10270055785075742	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	44	0.10270055785075742	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.10270055785075742	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGCGGAGGAATC	43	0.10036645426324019	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	43	0.10036645426324019	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	43	0.10036645426324019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.002334103587517214	0.0
16	0.0	0.0	0.0	0.002334103587517214	0.0
17	0.0	0.0	0.0	0.007002310762551642	0.0
18	0.0	0.0	0.0	0.009336414350068855	0.0
19	0.0	0.0	0.0	0.01167051793758607	0.0
20	0.0	0.0	0.0	0.014004621525103284	0.0
21	0.0	0.0	0.0	0.021006932287654927	0.0
22	0.0	0.0	0.0	0.030343346637723784	0.0
23	0.0	0.0	0.0	0.06068669327544757	0.0
24	0.0	0.0	0.0	0.10503466143827463	0.0
25	0.0	0.0	0.0	0.12137338655089513	0.0
26	0.0	0.0	0.0	0.14238031883855007	0.0
27	0.0	0.0	0.0	0.19139649417641155	0.0
28	0.0	0.0	0.0	0.34311322736503047	0.0
29	0.0	0.0	0.0	0.6162033471045445	0.0
30	0.0	0.0	0.0	1.0316737856826086	0.0
31	0.0	0.0	0.0	2.040006535490045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAAGAG	25	0.005438153	29.599998	22
ATGCCCC	50	8.827126E-6	25.899998	37
TTTTTGC	40	0.001901138	23.125002	1
ACCGACC	40	0.001901138	23.125002	32
CGCTTAT	105	3.6379788E-11	22.904764	26
TCCGCTT	105	3.6379788E-11	22.904764	24
CTCCGCT	105	3.6379788E-11	22.904764	23
CCGCTTA	110	6.730261E-11	21.863636	25
CCTCCGC	110	6.730261E-11	21.863636	22
GCTTATT	110	6.730261E-11	21.863636	27
TCCTCCG	105	9.058567E-10	21.142859	21
TTCCTCC	115	1.2369128E-10	20.913044	20
AGGGGAA	45	0.003766844	20.555555	10
AGAGGGG	45	0.003766844	20.555555	8
AGTGGCT	45	0.003766844	20.555555	27
TGGGAAC	55	5.032791E-4	20.181818	20
GGTATCA	600	0.0	20.041666	1
TGGGCAG	310	0.0	19.096773	6
GGCAGTC	315	0.0	18.79365	8
GCAGTCG	310	0.0	18.5	9
>>END_MODULE