FastQCFastQC Report
Fri 10 Feb 2017
ERR1631322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817340
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.4477940636699537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27550.33706902880074385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26280.32153081948760615No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11600.1419238016003132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.14106736486651822No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9460.11574130716715197No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9200.11256025644162772No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9110.11145912349817702No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8540.1044852815229892No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC8390.10265005995057136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTCG2700.022.611119
CAGTCGG3100.020.88709610
AGTCGGT3100.020.88709611
CAGTTCG450.003824876420.5555559
AACGACT450.003824876420.5555554
CCGCTTA759.260402E-619.73333425
CGCTTAT759.260402E-619.73333426
TCGGTGA3200.019.6562513
GGTATCA15550.019.1543391
AAGACGG2900.019.137935
ACGGACC3000.019.1166678
GCTTTAT3000.019.1166671
CGGACCA3100.019.0967759
CACTATC500.007033186518.49999832
ACAGCGT500.007033186518.4999988
TCCGGTC500.007033186518.4999988
CGGTGAT3450.018.23188414
CTCTATG5100.018.1372551
GTCGGTG3500.017.97142812
CGCCTTA2500.017.7625
Produced by FastQC (version 0.11.5)