##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631322.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	817340
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04898084028678	34.0	33.0	34.0	31.0	34.0
2	33.18647074656813	34.0	33.0	34.0	31.0	34.0
3	33.27059363300462	34.0	34.0	34.0	31.0	34.0
4	36.49518070815083	37.0	37.0	37.0	35.0	37.0
5	36.48353928597646	37.0	37.0	37.0	35.0	37.0
6	36.538616732326815	37.0	37.0	37.0	35.0	37.0
7	36.54729488340226	37.0	37.0	37.0	35.0	37.0
8	36.54860156116182	37.0	37.0	37.0	35.0	37.0
9	38.39167665842856	39.0	39.0	39.0	37.0	39.0
10	38.36071891746397	39.0	39.0	39.0	37.0	39.0
11	38.40984902243864	39.0	39.0	39.0	37.0	39.0
12	38.371484327207774	39.0	39.0	39.0	37.0	39.0
13	38.404080309296006	39.0	39.0	39.0	37.0	39.0
14	39.94019869332224	41.0	40.0	41.0	38.0	41.0
15	39.9281094770842	41.0	40.0	41.0	38.0	41.0
16	39.88627743656251	41.0	40.0	41.0	38.0	41.0
17	39.889529449188835	41.0	40.0	41.0	38.0	41.0
18	39.86712384075171	41.0	40.0	41.0	38.0	41.0
19	39.87376000195757	41.0	40.0	41.0	38.0	41.0
20	39.85351873149485	41.0	40.0	41.0	38.0	41.0
21	39.81953287493577	41.0	40.0	41.0	38.0	41.0
22	39.75406685100448	41.0	40.0	41.0	38.0	41.0
23	39.688197078327256	41.0	40.0	41.0	37.0	41.0
24	39.65580786453618	41.0	40.0	41.0	37.0	41.0
25	39.65471407247902	41.0	40.0	41.0	37.0	41.0
26	39.554371497785496	41.0	40.0	41.0	37.0	41.0
27	39.492804707955074	41.0	40.0	41.0	37.0	41.0
28	39.40583845156239	41.0	39.0	41.0	36.0	41.0
29	39.34350576259574	41.0	39.0	41.0	36.0	41.0
30	39.26261164264565	41.0	39.0	41.0	35.0	41.0
31	39.19527002226736	41.0	39.0	41.0	35.0	41.0
32	39.120886044975165	41.0	39.0	41.0	35.0	41.0
33	39.02310788655884	41.0	39.0	41.0	35.0	41.0
34	38.96265935840654	41.0	39.0	41.0	35.0	41.0
35	38.841086940563294	40.0	38.0	41.0	35.0	41.0
36	38.77778280764431	40.0	38.0	41.0	35.0	41.0
37	38.70353953067267	40.0	38.0	41.0	35.0	41.0
38	38.618018205398	40.0	38.0	41.0	35.0	41.0
39	38.51846355249957	40.0	38.0	41.0	35.0	41.0
40	38.39228472850956	40.0	38.0	41.0	35.0	41.0
41	38.2772909682629	40.0	37.0	41.0	35.0	41.0
42	38.128890057993004	40.0	37.0	41.0	34.0	41.0
43	37.29727530770548	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	0.0
14	5.0
15	2.0
16	3.0
17	11.0
18	15.0
19	29.0
20	58.0
21	113.0
22	192.0
23	347.0
24	469.0
25	809.0
26	1222.0
27	1682.0
28	2386.0
29	3339.0
30	4468.0
31	5714.0
32	7332.0
33	10063.0
34	14850.0
35	22079.0
36	36486.0
37	73449.0
38	157955.0
39	474260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45126874984707	17.263072895000857	11.96258594954364	26.32307240560844
2	20.112927300756112	19.694374434140016	31.675801991827147	28.516896273276725
3	20.832945897668047	21.154843761470136	28.406538282722	29.60567205813982
4	14.810849829936135	14.921207820490862	32.78660043555925	37.48134191401375
5	16.641055130056035	34.55673281620868	31.66992928279541	17.132282770939877
6	35.638901803411066	32.96363814324516	15.39139158734431	16.006068465999462
7	31.08363716446032	29.062201776494483	19.659015831844766	20.19514522720043
8	30.429441847945775	29.069542662784155	19.152127633542957	21.348887855727114
9	27.923263268651972	13.588836958915508	18.820074877040156	39.66782489539237
10	19.854528103359677	24.53825825237967	29.029534832505444	26.577678811755206
11	38.25323610737269	20.853500379279126	19.336873271833017	21.556390241515157
12	24.705875155993834	24.708199769985562	25.44461301294443	25.141312061076178
13	32.02265886901412	17.354711625516924	23.64169134020114	26.98093816526782
14	24.37798223505518	19.624880710597793	22.800303423299972	33.196833631047056
15	27.747326693909514	25.676585019698045	20.21337509481978	26.362713191572663
16	27.03452663518242	24.104656568869746	22.33256661854308	26.52825017740475
17	26.306310715247022	24.428022609929773	22.80837839821861	26.457288276604597
18	26.081801942887907	22.03342550223897	25.247999608486065	26.63677294638706
19	26.84904690826339	23.518731494849145	24.374434140015172	25.257787456872293
20	27.22734724839112	22.16813076565444	23.693689284752978	26.91083270120146
21	28.682188562899157	22.76384368806127	23.547972691903002	25.005995057136566
22	28.33117185014804	22.480607825384784	23.313186678738347	25.875033645728827
23	26.578779944698656	22.429099273252255	23.77700834414075	27.215112437908335
24	25.678297893165634	23.575133971174786	24.364034551104805	26.382533584554775
25	26.123400298529376	22.650671691095507	23.785939755793184	27.439988254581937
26	26.153375584212203	23.805148408251156	24.097682726894558	25.94379328064208
27	27.045415616512102	22.22012871020628	23.78838671788974	26.946068955391883
28	25.51643135047838	23.355152078694303	24.468030440208484	26.660386130618836
29	25.525852154550126	23.57843737000514	25.062030489147723	25.833679986297014
30	25.28531578045856	23.828761592482934	24.78270976582573	26.103212861232777
31	25.580174713093694	23.960530501382536	24.015831844764723	26.443462940759048
32	24.914111630410847	23.005970587515602	24.379328064208288	27.70058971786527
33	24.374189443805516	23.109599432304794	25.25962267844471	27.25658844544498
34	26.578535248489	22.279712237257446	24.828223260821687	26.313529253431867
35	25.70203342550224	22.42041255780948	25.5942447451489	26.283309271539384
36	23.959674064648738	23.55935106565199	25.76638852864169	26.71458634105758
37	24.790907088849192	21.88734186507451	26.48726356228742	26.834487483788877
38	24.324393765140577	21.529596006557856	27.02755279320723	27.118457435094328
39	24.437565762106345	20.975603787897327	27.498960041108962	27.087870408887365
40	23.550052609685075	21.46621968825703	28.16306555411457	26.82066214794333
41	22.14182592311645	21.569603836836567	29.661462793941322	26.627107446105658
42	23.02762620207013	21.540607335992366	29.5265128343162	25.90525362762131
43	21.867521472092395	20.85105341718257	29.62904054616194	27.652384564563093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	165.0
2	144.0
3	313.0
4	482.0
5	482.0
6	606.5
7	731.0
8	684.5
9	638.0
10	1019.0
11	1400.0
12	1400.0
13	2344.5
14	3289.0
15	5152.5
16	7016.0
17	6312.0
18	5608.0
19	5608.0
20	6156.5
21	6705.0
22	5424.5
23	4144.0
24	4522.0
25	4900.0
26	4900.0
27	5575.0
28	6250.0
29	7479.5
30	8709.0
31	10291.5
32	11874.0
33	11874.0
34	14549.5
35	17225.0
36	20670.5
37	24116.0
38	27431.5
39	30747.0
40	30747.0
41	33317.0
42	35887.0
43	38108.5
44	40330.0
45	43820.5
46	47311.0
47	47311.0
48	52261.5
49	57212.0
50	58333.0
51	59454.0
52	60940.5
53	62427.0
54	62427.0
55	60429.0
56	58431.0
57	58340.0
58	58249.0
59	58386.5
60	58524.0
61	58524.0
62	54913.0
63	51302.0
64	48204.5
65	45107.0
66	40185.5
67	35264.0
68	35264.0
69	31329.5
70	27395.0
71	23345.0
72	19295.0
73	14184.0
74	9073.0
75	9073.0
76	7228.5
77	5384.0
78	4652.5
79	3921.0
80	3472.0
81	3023.0
82	3023.0
83	2483.0
84	1943.0
85	1798.5
86	1654.0
87	1468.5
88	1283.0
89	1283.0
90	926.0
91	569.0
92	331.0
93	93.0
94	58.5
95	24.0
96	24.0
97	20.5
98	17.0
99	10.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	817340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.89871716121307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.64402426503848	46.0051114336294
2	5.853514013966002	6.075797364200372
3	1.61125959300197	2.508669176715016
4	0.8563087304279601	1.777652984126326
5	0.511129221229308	1.3263475442705481
6	0.3598693746016658	1.1206055332460692
7	0.26152048941106687	0.9500804538264884
8	0.20158196229671382	0.8369476196831575
9	0.16116381122581958	0.7527775549888751
>10	1.206804623270766	12.479253816698417
>50	0.1705517162291566	6.243989458034575
>100	0.1518640698999357	15.470550437670713
>500	0.009225387423725763	3.058090665545974
>1k	0.0011827419774007389	1.3941259573641138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3660	0.4477940636699537	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2755	0.33706902880074385	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2628	0.32153081948760615	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1160	0.1419238016003132	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1153	0.14106736486651822	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	946	0.11574130716715197	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	920	0.11256025644162772	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	911	0.11145912349817702	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	854	0.1044852815229892	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	839	0.10265005995057136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.223481048278562E-4	0.0
12	0.0	0.0	0.0	2.446962096557124E-4	0.0
13	0.0	0.0	0.0	2.446962096557124E-4	0.0
14	0.0	0.0	0.0	2.446962096557124E-4	0.0
15	0.0	0.0	0.0	6.117405241392811E-4	0.0
16	0.0	0.0	0.0	0.0012234810482785621	0.0
17	0.0	0.0	0.0	0.0018352215724178433	0.0
18	0.0	0.0	0.0	0.0023246139917292683	0.0
19	0.0	0.0	0.0	0.0029363545158685492	0.0
20	0.0	0.0	0.0	0.0036704431448356866	0.0
21	0.0	0.0	0.0	0.005016272297942105	0.0
22	0.0	0.0	0.0	0.006729145765532092	0.0
23	0.0	0.0	0.0	0.009543152176572785	0.0
24	0.0	0.0	0.0	0.015293513103482027	0.0
25	0.0	0.0	0.0	0.019331000562801282	0.0
26	0.0	0.0	0.0	0.028874152739374066	0.0
27	0.0	0.0	0.0	0.07756869846086084	0.0
28	0.0	1.223481048278562E-4	0.0	0.2687987863068001	0.0
29	0.0	1.223481048278562E-4	0.0	0.5246286735018475	0.0
30	0.0	1.223481048278562E-4	0.0	0.8291531064183816	0.0
31	0.0	1.223481048278562E-4	0.0	1.5521080578461839	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGTCG	270	0.0	22.61111	9
CAGTCGG	310	0.0	20.887096	10
AGTCGGT	310	0.0	20.887096	11
CAGTTCG	45	0.0038248764	20.555555	9
AACGACT	45	0.0038248764	20.555555	4
CCGCTTA	75	9.260402E-6	19.733334	25
CGCTTAT	75	9.260402E-6	19.733334	26
TCGGTGA	320	0.0	19.65625	13
GGTATCA	1555	0.0	19.154339	1
AAGACGG	290	0.0	19.13793	5
ACGGACC	300	0.0	19.116667	8
GCTTTAT	300	0.0	19.116667	1
CGGACCA	310	0.0	19.096775	9
CACTATC	50	0.0070331865	18.499998	32
ACAGCGT	50	0.0070331865	18.499998	8
TCCGGTC	50	0.0070331865	18.499998	8
CGGTGAT	345	0.0	18.231884	14
CTCTATG	510	0.0	18.137255	1
GTCGGTG	350	0.0	17.971428	12
CGCCTTA	250	0.0	17.76	25
>>END_MODULE