FastQCFastQC Report
Fri 10 Feb 2017
ERR1631321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480204
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9110.18971103947488982No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7650.15930729439988003No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC7380.15368468400929605No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6500.13535913903257782No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6240.1299447734712747No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6190.12890354932487028No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC5940.12369742859284803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3650.024.8356171
CCGCTTA1650.021.3030325
CGCTTAT1650.021.3030326
GTATTAG1303.274181E-1119.9230771
GCAGTCG4750.018.6947369
ACTATCC609.226143E-418.58
GCGAAAG609.226143E-418.518
TAGAACT609.226143E-418.54
TGATTGA500.00702906318.56
TCTAATA609.226143E-418.52
TACTATA609.226143E-418.52
TGTAGGA701.217322E-418.52
TCTTTCG701.217322E-418.510
TATACTC609.226143E-418.55
CTCTATG7400.018.51
TCGGTGA4800.018.513
AGTCGGT5050.018.31683211
CTTATTG4750.018.30526428
CAGTCGG5150.017.6019410
GGCAGTC5050.017.584168
Produced by FastQC (version 0.11.5)