##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480204
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02763200639728	34.0	33.0	34.0	31.0	34.0
2	33.18914669598754	34.0	33.0	34.0	31.0	34.0
3	33.26753837952204	34.0	34.0	34.0	31.0	34.0
4	36.5045751388993	37.0	37.0	37.0	35.0	37.0
5	36.494918826165545	37.0	37.0	37.0	35.0	37.0
6	36.542604809622574	37.0	37.0	37.0	35.0	37.0
7	36.54374599128704	37.0	37.0	37.0	35.0	37.0
8	36.53934369559604	37.0	37.0	37.0	35.0	37.0
9	38.40081298781351	39.0	39.0	39.0	37.0	39.0
10	38.348487309560106	39.0	39.0	39.0	37.0	39.0
11	38.40933228377939	39.0	39.0	39.0	37.0	39.0
12	38.35490541519854	39.0	39.0	39.0	37.0	39.0
13	38.39054235283338	39.0	39.0	39.0	37.0	39.0
14	39.928936452007896	41.0	40.0	41.0	38.0	41.0
15	39.93362196066672	41.0	40.0	41.0	38.0	41.0
16	39.90080465802034	41.0	40.0	41.0	38.0	41.0
17	39.863485102164915	41.0	40.0	41.0	38.0	41.0
18	39.84438072152668	41.0	40.0	41.0	38.0	41.0
19	39.863143580644895	41.0	40.0	41.0	38.0	41.0
20	39.84800418155617	41.0	40.0	41.0	38.0	41.0
21	39.81645925481671	41.0	40.0	41.0	38.0	41.0
22	39.767463411383495	41.0	40.0	41.0	38.0	41.0
23	39.699508958692554	41.0	40.0	41.0	37.0	41.0
24	39.68178107637587	41.0	40.0	41.0	37.0	41.0
25	39.65850971670373	41.0	40.0	41.0	37.0	41.0
26	39.570949013336	41.0	40.0	41.0	37.0	41.0
27	39.51523727415848	41.0	39.0	41.0	37.0	41.0
28	39.42602102439796	41.0	39.0	41.0	37.0	41.0
29	39.37451999566851	41.0	39.0	41.0	36.0	41.0
30	39.31091161256466	41.0	39.0	41.0	36.0	41.0
31	39.26638470316782	41.0	39.0	41.0	35.0	41.0
32	39.18932995143731	41.0	39.0	41.0	35.0	41.0
33	39.11677120557096	41.0	39.0	41.0	35.0	41.0
34	39.0538916793696	41.0	39.0	41.0	35.0	41.0
35	38.95728898551449	40.0	39.0	41.0	35.0	41.0
36	38.895915069428824	40.0	39.0	41.0	35.0	41.0
37	38.830703201139514	40.0	38.0	41.0	35.0	41.0
38	38.75957717969863	40.0	38.0	41.0	35.0	41.0
39	38.67044422786982	40.0	38.0	41.0	35.0	41.0
40	38.58029712372242	40.0	38.0	41.0	35.0	41.0
41	38.483744408626336	40.0	37.0	41.0	35.0	41.0
42	38.3624771971912	40.0	37.0	41.0	35.0	41.0
43	37.51800068304304	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	4.0
18	8.0
19	14.0
20	19.0
21	51.0
22	101.0
23	165.0
24	261.0
25	378.0
26	552.0
27	812.0
28	1141.0
29	1652.0
30	2186.0
31	2808.0
32	3738.0
33	5255.0
34	8410.0
35	12775.0
36	21858.0
37	41483.0
38	102349.0
39	274182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49047488150869	16.28578687391192	11.707107812513016	28.51663043206637
2	21.346136225437522	17.947580611573414	31.394365727898975	29.311917435090084
3	20.893828456239433	19.37718136458672	27.163247286569874	32.56574289260398
4	15.489250401912521	14.57047421512524	31.649049154109505	38.291226228852736
5	17.847206603860027	33.42870946514398	31.45892162497605	17.265162306019942
6	38.12587983440371	31.52930837727299	14.694796378205929	15.650015410117366
7	33.28377106396448	26.918976101823393	18.854486843091685	20.942765991120442
8	30.611989904290677	29.64094426535389	17.67936127145963	22.067704558895805
9	28.238831829805665	13.26373791138766	17.1479204671348	41.34950979167187
10	19.79450400246562	23.290309951603902	28.93728498721377	27.977901058716714
11	40.438022173909424	19.71058133626542	17.99797586025939	21.85342062956577
12	25.369634571973577	22.491899276140973	25.104538904299005	27.03392724758644
13	33.73274691589408	16.18520462136925	22.127054335240857	27.954994127495812
14	24.99729281721935	18.00026655338148	21.042307019516706	35.96013360988247
15	29.712996976285076	23.811963248952527	18.528583685267094	27.946456089495296
16	29.287344545234944	22.46503569316374	20.399455231526602	27.84816453007472
17	27.650956676745714	22.850913361821227	21.382370825732398	28.115759135700664
18	28.48122881108862	20.578545784708165	22.679944356981615	28.260281047221596
19	28.25736561961166	22.59643818043998	21.466709981591155	27.679486218357198
20	28.53953736328727	20.990245812196484	21.826765291417814	28.643451533098435
21	30.111994069187265	21.35321654963307	21.340097125388375	27.194692255791285
22	30.113035293333667	20.714946147887147	20.67017350959176	28.50184504918743
23	28.185521153509757	20.83052202813804	21.58999092052544	29.393965897826757
24	27.737586525726567	21.791571915269344	21.600611406818768	28.87023015218532
25	28.127212601311108	20.951720518779517	21.424228036417855	29.496838843491517
26	28.805466010278963	21.85654430200498	21.325936476997278	28.01205321071878
27	28.812546334474515	20.869255566384286	21.389659394757228	28.92853870438397
28	27.86669831988072	21.23805715904074	22.131219231826474	28.764025289252064
29	26.699694296590614	21.77220514614622	23.26407110311451	28.264029454148655
30	26.285703575980207	22.76428351284038	23.23637454082015	27.713638370359263
31	28.22592065039025	22.17744958392683	21.174125996451508	28.42250376923141
32	26.882325011869955	21.73909421829056	22.442337006772124	28.936243763067367
33	26.739052569324706	20.97983357073244	22.970862383487017	29.31025147645584
34	28.053493931745678	21.026063922832797	22.672447543127504	28.247994602294025
35	27.646375290501535	21.193909255233194	23.424627866490074	27.735087587775197
36	26.11098616421354	21.916310568008594	23.51458963273942	28.45811363503844
37	27.063289768515048	20.325320072302606	24.32820218073985	28.2831879784425
38	25.647641419063564	19.768056909146946	25.68637495730981	28.897926714479677
39	26.196991278706548	19.30512865365553	26.10765424694505	28.390225820692873
40	25.350267802850457	19.562102772988148	26.455423111844134	28.632206312317265
41	23.80342521095201	19.863849530616154	28.15532565326403	28.177399605167803
42	24.14203130336274	19.843025047688066	28.16698736370376	27.84795628524544
43	22.5547892145838	19.540653555572217	28.823791555255685	29.0807656745883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	13.5
4	25.0
5	25.0
6	36.5
7	48.0
8	38.5
9	29.0
10	66.0
11	103.0
12	103.0
13	187.5
14	272.0
15	408.5
16	545.0
17	506.5
18	468.0
19	468.0
20	565.0
21	662.0
22	548.5
23	435.0
24	543.5
25	652.0
26	652.0
27	872.0
28	1092.0
29	1509.0
30	1926.0
31	2582.5
32	3239.0
33	3239.0
34	4439.0
35	5639.0
36	7294.5
37	8950.0
38	11063.0
39	13176.0
40	13176.0
41	15079.5
42	16983.0
43	19445.5
44	21908.0
45	24479.5
46	27051.0
47	27051.0
48	31072.0
49	35093.0
50	37235.5
51	39378.0
52	40069.0
53	40760.0
54	40760.0
55	40143.5
56	39527.0
57	39301.0
58	39075.0
59	39080.5
60	39086.0
61	39086.0
62	37623.5
63	36161.0
64	34133.5
65	32106.0
66	28766.0
67	25426.0
68	25426.0
69	21847.0
70	18268.0
71	15664.5
72	13061.0
73	10028.5
74	6996.0
75	6996.0
76	5685.5
77	4375.0
78	3667.5
79	2960.0
80	2552.5
81	2145.0
82	2145.0
83	1658.5
84	1172.0
85	948.0
86	724.0
87	565.0
88	406.0
89	406.0
90	306.5
91	207.0
92	127.5
93	48.0
94	32.0
95	16.0
96	16.0
97	10.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	480204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.56450722947697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1744333636994	56.68328914231384
2	5.987087241480715	7.611325004892206
3	1.687964999362458	3.2188399021523715
4	0.7612468959433276	1.9355313528242626
5	0.45754308877692673	1.4541750487179093
6	0.3200378215495961	1.2205827852957218
7	0.22762921631565383	1.0128397276295589
8	0.16299270210276695	0.8288440632930655
9	0.12815440989853685	0.7331464723036413
>10	0.9142027683037677	11.431596303179779
>50	0.10531564953334432	4.666789932780462
>100	0.07108806320727015	8.177760660669044
>500	0.0023037798261615326	1.0252796039481973
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	911	0.18971103947488982	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	765	0.15930729439988003	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	738	0.15368468400929605	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	650	0.13535913903257782	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	624	0.1299447734712747	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	619	0.12890354932487028	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	594	0.12369742859284803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.0824482928088895E-4	0.0
5	0.0	0.0	0.0	2.0824482928088895E-4	0.0
6	0.0	0.0	0.0	2.0824482928088895E-4	0.0
7	0.0	0.0	0.0	2.0824482928088895E-4	0.0
8	0.0	0.0	0.0	2.0824482928088895E-4	0.0
9	0.0	0.0	0.0	2.0824482928088895E-4	0.0
10	0.0	0.0	0.0	4.164896585617779E-4	0.0
11	0.0	0.0	0.0	4.164896585617779E-4	0.0
12	0.0	0.0	0.0	4.164896585617779E-4	0.0
13	0.0	0.0	0.0	4.164896585617779E-4	0.0
14	0.0	0.0	0.0	4.164896585617779E-4	0.0
15	0.0	0.0	0.0	4.164896585617779E-4	0.0
16	0.0	0.0	0.0	0.0010412241464044449	0.0
17	0.0	0.0	0.0	0.0012494689756853336	0.0
18	0.0	0.0	0.0	0.0012494689756853336	0.0
19	0.0	0.0	0.0	0.0014577138049662226	0.0
20	0.0	0.0	0.0	0.0016659586342471116	0.0
21	0.0	0.0	0.0	0.0027071827806515562	0.0
22	0.0	0.0	0.0	0.003331917268494223	0.0
23	0.0	0.0	0.0	0.005206120732022224	0.0
24	0.0	0.0	0.0	0.007705058683392892	0.0
25	0.0	0.0	0.0	0.008954527659078225	0.0
26	0.0	0.0	0.0	0.012494689756853337	0.0
27	0.0	0.0	0.0	0.035401620977751125	0.0
28	0.0	4.164896585617779E-4	0.0	0.13889930113035293	2.0824482928088895E-4
29	0.0	4.164896585617779E-4	0.0	0.309451816311401	4.164896585617779E-4
30	0.0	4.164896585617779E-4	0.0	0.500828814420538	4.164896585617779E-4
31	0.0	4.164896585617779E-4	0.0	1.0803741743092519	4.164896585617779E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	365	0.0	24.835617	1
CCGCTTA	165	0.0	21.30303	25
CGCTTAT	165	0.0	21.30303	26
GTATTAG	130	3.274181E-11	19.923077	1
GCAGTCG	475	0.0	18.694736	9
ACTATCC	60	9.226143E-4	18.5	8
GCGAAAG	60	9.226143E-4	18.5	18
TAGAACT	60	9.226143E-4	18.5	4
TGATTGA	50	0.007029063	18.5	6
TCTAATA	60	9.226143E-4	18.5	2
TACTATA	60	9.226143E-4	18.5	2
TGTAGGA	70	1.217322E-4	18.5	2
TCTTTCG	70	1.217322E-4	18.5	10
TATACTC	60	9.226143E-4	18.5	5
CTCTATG	740	0.0	18.5	1
TCGGTGA	480	0.0	18.5	13
AGTCGGT	505	0.0	18.316832	11
CTTATTG	475	0.0	18.305264	28
CAGTCGG	515	0.0	17.60194	10
GGCAGTC	505	0.0	17.58416	8
>>END_MODULE