FastQCFastQC Report
Fri 10 Feb 2017
ERR1631320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601076
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6960.11579234572666351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTT250.005494282629.67
TATTACA358.864452E-426.4285722
GGTATCA3950.022.0126571
ATACACA3100.021.48387137
AGTAGTG705.095242E-621.1428596
CGACGGT1052.2540917E-819.380957
ATTACAC500.007031072418.53
TCTAGCA609.2298293E-418.53
GTAGCAC609.2298293E-418.53
GCTAGAC500.007031072418.53
CAGTAGT801.6145805E-518.55
TTATTAC500.007031072418.51
CGGACGA609.2298293E-418.514
CTTATAC13750.018.0290937
CGGTCCA1156.389746E-817.69565210
GACGGTA953.6005968E-617.5263168
GCGATCG852.7196178E-517.41176413
CACATGT752.0651997E-417.26666628
TATTAGA752.0651997E-417.2666662
CCGGTCT650.001578748317.0769239
Produced by FastQC (version 0.11.5)