FastQCFastQC Report
Fri 10 Feb 2017
ERR1631319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961714
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT492385.119817326148938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT361703.760993393046166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346783.6058537153457264No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130351.3553925595343315No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA56150.5838534117211562No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49680.5165776935762607No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43030.4474303171213063No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA40150.41748378416036364No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA36200.37641128235629306No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA35880.3730838898050772No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33060.3437612429474875No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32670.3397059832756932No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31440.32691631815695726No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30170.31371072896931934No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA27160.2824124427844453No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA26230.2727422081824742No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA25290.26296799256327763No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA22590.2348931179123939No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA22040.22917416196499169No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT21550.2240790921209424No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.22095966160417757No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.21981587041469708No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT21020.21856809820799114No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT20080.20879388258879458No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT19840.20629833817538268No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT18570.19309274898774478No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC17890.1860220398164111No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA17590.18290260929964627No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC17220.17905531166230293No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA16270.16917711502588087No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.1682412858708514No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA16140.16782536180194943No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15680.16304223500957665No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT15650.16273029195790017No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG15090.1569073549932724No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14980.15576356380379197No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC14710.1529560763387036No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA14010.14567740513291894No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT13920.14474157597788948No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA13900.14453361394343847No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA13150.13673503765152634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC12260.12748072711845726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC12150.12633693592897682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12070.12550508779117286No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA11980.12456925863614339No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA11250.11697864437868223No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG11160.11604281522365277No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA10870.11302736572411341No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT10770.11198755555185845No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA10700.11125968843127998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10690.11115570741405448No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.10751637181116215No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA9990.10387703620826981No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT9990.10387703620826981No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT9810.1020053778982109No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA9740.10127751077763243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT355.630027E-737.01
CGTATGC250.00549590229.631
TGGATAC250.00549590229.627
CTCTAAG250.00549590229.61
GCGTATA250.00549590229.61
CTTATTC405.9391357E-527.75000228
GTTATAC405.9391357E-527.7500023
ATGCCCC3850.027.3896137
CGTGATA754.620233E-1027.1333332
TGATAAG358.868337E-426.4285725
ATTGACT3550.025.01408631
TATTGAC3650.024.32876830
TATGCTG1850.024.036
CCGTTTA1250.023.6827
TTTGAGC551.9022398E-523.5454543
GATAAGT400.001930958923.1250026
CTTACTG400.001930958923.12500228
GCAGCGT2100.022.904761
GATACAC1950.022.7692323
ATACACT1950.022.7692324
Produced by FastQC (version 0.11.5)