##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631319.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961714
Sequences flagged as poor quality	0
Sequence length	43
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88536196831907	34.0	33.0	34.0	31.0	34.0
2	32.91861405781761	34.0	33.0	34.0	31.0	34.0
3	32.91044530910437	34.0	33.0	34.0	31.0	34.0
4	36.2794271477799	37.0	37.0	37.0	35.0	37.0
5	36.30866557001354	37.0	37.0	37.0	35.0	37.0
6	36.45266680114878	37.0	37.0	37.0	35.0	37.0
7	36.44335114181555	37.0	37.0	37.0	35.0	37.0
8	36.49343047933169	37.0	37.0	37.0	35.0	37.0
9	38.254635993653	39.0	39.0	39.0	37.0	39.0
10	38.240935454823365	39.0	39.0	39.0	37.0	39.0
11	38.30852415583011	39.0	39.0	39.0	37.0	39.0
12	38.30382733328204	39.0	39.0	39.0	37.0	39.0
13	38.3237927283995	39.0	39.0	39.0	37.0	39.0
14	39.584481457065195	41.0	39.0	41.0	37.0	41.0
15	39.49421761563209	41.0	39.0	41.0	37.0	41.0
16	39.41879914402826	40.0	39.0	41.0	37.0	41.0
17	39.58737732839493	41.0	39.0	41.0	37.0	41.0
18	39.750138814657994	41.0	40.0	41.0	38.0	41.0
19	39.81338734800575	41.0	40.0	41.0	38.0	41.0
20	39.81222588004334	41.0	40.0	41.0	38.0	41.0
21	39.7682439893773	41.0	40.0	41.0	37.0	41.0
22	39.64318705977037	41.0	40.0	41.0	37.0	41.0
23	39.53166949841637	41.0	40.0	41.0	35.0	41.0
24	39.46146879425692	41.0	39.0	41.0	35.0	41.0
25	39.41760648176069	41.0	39.0	41.0	35.0	41.0
26	39.141061687778276	41.0	39.0	41.0	35.0	41.0
27	38.94018491984103	41.0	38.0	41.0	35.0	41.0
28	38.73231750811572	40.0	37.0	41.0	35.0	41.0
29	38.43089109652142	40.0	37.0	41.0	35.0	41.0
30	38.024267089800084	40.0	35.0	41.0	35.0	41.0
31	37.552769326431765	39.0	35.0	41.0	35.0	41.0
32	37.15625747363561	39.0	35.0	41.0	35.0	41.0
33	36.80215011947419	38.0	35.0	41.0	34.0	41.0
34	36.502945782218	38.0	35.0	41.0	33.0	41.0
35	36.16096989333627	38.0	35.0	41.0	33.0	41.0
36	35.792490282973944	37.0	35.0	41.0	32.0	41.0
37	35.40288380953173	37.0	35.0	41.0	31.0	41.0
38	34.98928371636474	36.0	35.0	41.0	25.0	41.0
39	34.49561096126291	35.0	35.0	41.0	21.0	41.0
40	33.97813071245714	35.0	35.0	41.0	15.0	41.0
41	33.37783270286177	35.0	35.0	41.0	10.0	41.0
42	32.72753750075386	35.0	34.0	41.0	8.0	41.0
43	31.71578140694635	35.0	33.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	25.0
11	21.0
12	31.0
13	24.0
14	27.0
15	23.0
16	28.0
17	48.0
18	69.0
19	90.0
20	183.0
21	275.0
22	536.0
23	838.0
24	1397.0
25	2398.0
26	3733.0
27	6209.0
28	9919.0
29	14110.0
30	17684.0
31	20044.0
32	22151.0
33	26622.0
34	34942.0
35	45420.0
36	69132.0
37	212088.0
38	170312.0
39	303328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.039824729597356	14.39263648028416	17.305560696839184	18.26197809327929
2	16.95233718132418	25.995982173494408	33.984323821843084	23.06735682333833
3	20.131972707062598	29.09316075257301	30.582792805345456	20.192073735018933
4	11.350463859317843	19.9374242238337	29.842759905751603	38.86935201109685
5	10.11007430483491	44.96981431069944	28.29448255926398	16.625628825201673
6	23.993411762748593	33.266438878918265	21.930220418960314	20.809928939372828
7	25.798210278731514	36.269514637407795	18.891063247493538	19.041211836367154
8	37.135260586827265	27.43695111020532	17.583293993848486	17.84449430911893
9	32.847915284585646	11.725939312519106	19.01355288578517	36.41259251711008
10	21.472392000116457	32.491156414484976	21.436414568156437	24.600037017242133
11	29.916378465947258	29.28178231782006	15.760714723920003	25.041124492312683
12	23.98311764204327	36.0492828429242	24.94525399443078	15.02234552060176
13	35.135705625581	20.99407932087918	26.769912884703768	17.100302168836055
14	23.24578824889728	23.942876988377	31.41297724687381	21.398357515851906
15	28.569720311859864	27.734128857435785	26.315931763497257	17.38021906720709
16	17.18515067889206	28.858267634660617	31.46611154667604	22.490470139771286
17	14.2753458928538	34.42437148674138	31.049771553705156	20.250511066699666
18	16.722955057324736	24.519867652961274	37.81415264829253	20.943024641421463
19	17.056214217532446	30.723167178599876	40.27267981957214	11.94793878429554
20	20.12448607382236	24.73874769422094	41.315505441326636	13.821260790630063
21	23.301834017181825	24.03500416963879	38.259607326086545	14.403554487092837
22	20.784869514221484	25.471085998540104	35.07643644576246	18.667608041475948
23	16.795533807348132	28.71123847630377	36.09649022474457	18.39673749160353
24	15.328049711244715	28.965887987489005	39.759429518547094	15.946632782719186
25	14.748459521229803	28.634812428643023	37.54089053502393	19.07583751510324
26	18.078659559910744	27.52523099382977	37.392301661408695	17.003807784850796
27	16.200034521697717	28.376939505923797	36.30008505647209	19.12294091590639
28	14.345013174394882	29.27096829202861	39.60387391677776	16.780144616798758
29	14.59945472354567	30.05924838361509	40.296075548447874	15.045221344391368
30	13.83748182931724	31.005995545453224	41.757528745552214	13.398993879677326
31	14.350004263221706	32.580684070316124	38.03823174041347	15.031079926048701
32	13.4249891339837	32.269780828811896	40.81202935592078	13.493200681283623
33	14.74741971105755	30.746874850527288	40.17888894203474	14.32681649638042
34	14.872612855797046	31.922484231278737	35.7340123986965	17.470890514227722
35	13.597077717491896	33.17763909020769	36.121133725827015	17.1041494664734
36	11.792071239474522	32.79925216852411	37.20108057073101	18.207596021270355
37	12.830009753419416	33.023643203696736	37.19546559580083	16.95088144708302
38	13.67724708177275	29.99727569734869	38.9054334240741	17.420043796804453
39	14.196008376710747	30.30079628662991	38.56666326995344	16.936532066705904
40	13.199974212707726	30.178722572407178	36.67129728796711	19.95000592691798
41	10.815481525692668	31.21790885855878	38.37024312841448	19.596366487334073
42	11.067115587378368	30.37015162511932	37.31161239204171	21.251120395460603
43	10.43231147721672	32.53805185325367	38.09417352768079	18.935463141848825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	286.0
2	490.0
3	2453.5
4	4417.0
5	4417.0
6	6336.0
7	8255.0
8	8427.0
9	8599.0
10	13527.5
11	18456.0
12	18456.0
13	30639.5
14	42823.0
15	68051.0
16	93279.0
17	81552.5
18	69826.0
19	69826.0
20	74160.0
21	78494.0
22	54420.5
23	30347.0
24	26356.0
25	22365.0
26	22365.0
27	20072.5
28	17780.0
29	16948.5
30	16117.0
31	15306.0
32	14495.0
33	14495.0
34	13878.0
35	13261.0
36	12748.0
37	12235.0
38	13864.0
39	15493.0
40	15493.0
41	19041.5
42	22590.0
43	29441.0
44	36292.0
45	47067.0
46	57842.0
47	57842.0
48	74091.5
49	90341.0
50	83936.5
51	77532.0
52	59291.0
53	41050.0
54	41050.0
55	32882.5
56	24715.0
57	23006.5
58	21298.0
59	20838.5
60	20379.0
61	20379.0
62	20399.5
63	20420.0
64	19851.0
65	19282.0
66	18448.0
67	17614.0
68	17614.0
69	16236.0
70	14858.0
71	13246.0
72	11634.0
73	9839.0
74	8044.0
75	8044.0
76	6673.5
77	5303.0
78	4201.5
79	3100.0
80	2355.0
81	1610.0
82	1610.0
83	1129.5
84	649.0
85	446.5
86	244.0
87	162.0
88	80.0
89	80.0
90	49.5
91	19.0
92	10.5
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	961714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.15385052021266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.21963123245338	29.606443272130996
2	8.311410575854932	5.843561699914377
3	2.396426393037925	2.5273084561064274
4	1.1484754860275541	1.6149334224776486
5	0.6987482119953335	1.2281845097874908
6	0.4779198796009713	1.0080434404878347
7	0.3455613796594566	0.8503469160274894
8	0.27829426259595075	0.7826491926344689
9	0.2180557917600591	0.6898950637739762
>10	1.5114790306256705	10.550403912956696
>50	0.198626978450166	4.876305332186734
>100	0.15925678565302667	11.069399518872796
>500	0.02072114311470545	4.97079245132746
>1k	0.01391276751987366	9.944706736802045
>5k	2.960163302100779E-4	0.5843032912033933
>10k+	0.0011840653208403116	13.852722783310226
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49238	5.119817326148938	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36170	3.760993393046166	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34678	3.6058537153457264	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13035	1.3553925595343315	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	5615	0.5838534117211562	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4968	0.5165776935762607	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4303	0.4474303171213063	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	4015	0.41748378416036364	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3620	0.37641128235629306	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	3588	0.3730838898050772	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3306	0.3437612429474875	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3267	0.3397059832756932	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3144	0.32691631815695726	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3017	0.31371072896931934	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	2716	0.2824124427844453	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2623	0.2727422081824742	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	2529	0.26296799256327763	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	2259	0.2348931179123939	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	2204	0.22917416196499169	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	2155	0.2240790921209424	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	0.22095966160417757	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2114	0.21981587041469708	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	2102	0.21856809820799114	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	2008	0.20879388258879458	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	1984	0.20629833817538268	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	1857	0.19309274898774478	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	1789	0.1860220398164111	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	1759	0.18290260929964627	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	1722	0.17905531166230293	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1627	0.16917711502588087	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1618	0.1682412858708514	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	1614	0.16782536180194943	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1568	0.16304223500957665	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	1565	0.16273029195790017	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	1509	0.1569073549932724	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1498	0.15576356380379197	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	1471	0.1529560763387036	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	1401	0.14567740513291894	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	1392	0.14474157597788948	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	1390	0.14453361394343847	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	1315	0.13673503765152634	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	1226	0.12748072711845726	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	1215	0.12633693592897682	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1207	0.12550508779117286	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1198	0.12456925863614339	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA	1125	0.11697864437868223	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	1116	0.11604281522365277	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	1087	0.11302736572411341	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	1077	0.11198755555185845	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	1070	0.11125968843127998	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1069	0.11115570741405448	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1034	0.10751637181116215	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	999	0.10387703620826981	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	999	0.10387703620826981	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	981	0.1020053778982109	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	974	0.10127751077763243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.0796203445099063E-4	0.0
2	0.0	0.0	0.0	4.1592406890198126E-4	0.0
3	0.0	0.0	0.0	4.1592406890198126E-4	0.0
4	0.0	0.0	0.0	4.1592406890198126E-4	1.0398101722549531E-4
5	0.0	0.0	0.0	4.1592406890198126E-4	1.0398101722549531E-4
6	0.0	0.0	0.0	4.1592406890198126E-4	1.0398101722549531E-4
7	0.0	0.0	0.0	5.199050861274765E-4	1.0398101722549531E-4
8	0.0	0.0	0.0	5.199050861274765E-4	1.0398101722549531E-4
9	0.0	0.0	0.0	0.0022875823789608968	1.0398101722549531E-4
10	0.0	0.0	0.0	0.009150329515843587	2.0796203445099063E-4
11	0.0	0.0	0.0	0.02235591870348149	2.0796203445099063E-4
12	0.0	0.0	0.0	0.0399287106145902	2.0796203445099063E-4
13	0.0	0.0	0.0	0.04450387537251199	2.0796203445099063E-4
14	0.0	0.0	0.0	0.055109939129512515	2.0796203445099063E-4
15	0.0	0.0	0.0	0.07538623748848411	2.0796203445099063E-4
16	0.0	0.0	0.0	0.11427513793081935	2.0796203445099063E-4
17	0.0	0.0	0.0	0.15888299432055683	2.0796203445099063E-4
18	0.0	0.0	0.0	0.1794712357312049	2.0796203445099063E-4
19	0.0	0.0	0.0	0.20557047105480422	2.0796203445099063E-4
20	0.0	0.0	0.0	0.24009216877366868	2.0796203445099063E-4
21	0.0	0.0	0.0	0.29426627874815176	2.0796203445099063E-4
22	0.0	0.0	0.0	0.4124926953335399	2.0796203445099063E-4
23	0.0	0.0	0.0	0.5696080123612633	2.0796203445099063E-4
24	0.0	0.0	0.0	0.7594773498150178	2.0796203445099063E-4
25	0.0	0.0	0.0	0.8163549662373637	2.0796203445099063E-4
26	0.0	0.0	0.0	0.8784316335209844	3.119430516764859E-4
27	0.0	0.0	0.0	0.9572492445779098	3.119430516764859E-4
28	0.0	0.0	0.0	1.186111463491225	3.119430516764859E-4
29	0.0	0.0	0.0	1.6028673805310103	5.199050861274765E-4
30	0.0	0.0	0.0	2.288934132184828	5.199050861274765E-4
31	0.0	0.0	0.0	3.316890468476075	5.199050861274765E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	35	5.630027E-7	37.0	1
CGTATGC	25	0.005495902	29.6	31
TGGATAC	25	0.005495902	29.6	27
CTCTAAG	25	0.005495902	29.6	1
GCGTATA	25	0.005495902	29.6	1
CTTATTC	40	5.9391357E-5	27.750002	28
GTTATAC	40	5.9391357E-5	27.750002	3
ATGCCCC	385	0.0	27.38961	37
CGTGATA	75	4.620233E-10	27.133333	2
TGATAAG	35	8.868337E-4	26.428572	5
ATTGACT	355	0.0	25.014086	31
TATTGAC	365	0.0	24.328768	30
TATGCTG	185	0.0	24.0	36
CCGTTTA	125	0.0	23.68	27
TTTGAGC	55	1.9022398E-5	23.545454	3
GATAAGT	40	0.0019309589	23.125002	6
CTTACTG	40	0.0019309589	23.125002	28
GCAGCGT	210	0.0	22.90476	1
GATACAC	195	0.0	22.769232	3
ATACACT	195	0.0	22.769232	4
>>END_MODULE