ERR1631318.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40582
Sequences flagged as poor quality	0
Sequence length	43
%GC	50

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	707	1.7421516928687597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	611	1.5055936129318417	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	522	1.2862845596569907	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.4435463998817209	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	157	0.38687102656350103	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	94	0.23162978660489875	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.1995958799467744	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	79	0.1946675866147553	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	79	0.1946675866147553	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.1946675866147553	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	67	0.16509782662264058	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	67	0.16509782662264058	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.16263367995663103	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	63	0.15524123995860234	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	63	0.15524123995860234	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	62	0.15277709329259279	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	55	0.13552806663052586	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.13306391996451628	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	53	0.13059977329850672	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.12567147996648761	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	50	0.12320733330047805	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	47	0.11581489330244937	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.11088659997043022	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	43	0.1059583066384111	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	41	0.101030013306392	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.101030013306392	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCGC	115	1.2187229E-10	20.913044	21
GCCTTAT	115	1.2187229E-10	20.913044	26
CCTTATT	120	2.1645974E-10	20.041668	27
TCGCCTT	115	2.8030627E-9	19.304348	24
CTTATAC	115	2.8030627E-9	19.304348	37
TTCCTCG	125	3.7289283E-10	19.24	20
CTTATTG	145	7.2759576E-12	19.13793	28
GGTGATT	155	0.0	19.096775	15
GTGATTC	155	0.0	19.096775	16
CGCCTTA	120	4.7548383E-9	18.5	25
CCTCGCC	120	4.7548383E-9	18.5	22
TTGATAT	120	4.7548383E-9	18.5	32
GATTCCT	160	1.8189894E-12	18.5	18
TGGGCAG	160	1.8189894E-12	18.5	6
GGGCAGT	165	1.8189894E-12	17.939394	7
TGATTCC	165	1.8189894E-12	17.939394	17
GGCAGTC	165	1.8189894E-12	17.939394	8
GTCGGTG	165	1.8189894E-12	17.939394	12
ATTCCTC	155	1.8189894E-11	17.903227	19
GATATGC	125	7.887138E-9	17.76	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content