##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40582
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.916563993888914	34.0	33.0	34.0	31.0	34.0
2	33.01564733132916	34.0	33.0	34.0	31.0	34.0
3	33.065792715982454	34.0	33.0	34.0	31.0	34.0
4	36.350105958306635	37.0	37.0	37.0	35.0	37.0
5	36.36195850377015	37.0	37.0	37.0	35.0	37.0
6	36.44630624414765	37.0	37.0	37.0	35.0	37.0
7	36.451924498546155	37.0	37.0	37.0	35.0	37.0
8	36.467670395741955	37.0	37.0	37.0	35.0	37.0
9	38.28892119658962	39.0	39.0	39.0	37.0	39.0
10	38.26819772312848	39.0	39.0	39.0	37.0	39.0
11	38.30737765511803	39.0	39.0	39.0	37.0	39.0
12	38.29370164112168	39.0	39.0	39.0	37.0	39.0
13	38.30476565965206	39.0	39.0	39.0	37.0	39.0
14	39.722315312207385	41.0	40.0	41.0	37.0	41.0
15	39.70395741954561	41.0	40.0	41.0	37.0	41.0
16	39.619461830368145	41.0	39.0	41.0	37.0	41.0
17	39.66635454142231	41.0	40.0	41.0	37.0	41.0
18	39.71243408407668	41.0	40.0	41.0	38.0	41.0
19	39.74533044206791	41.0	40.0	41.0	38.0	41.0
20	39.72128037060766	41.0	40.0	41.0	38.0	41.0
21	39.69700852594747	41.0	40.0	41.0	37.0	41.0
22	39.61340002956976	41.0	40.0	41.0	37.0	41.0
23	39.55593612931842	41.0	40.0	41.0	37.0	41.0
24	39.48494406387068	41.0	40.0	41.0	36.0	41.0
25	39.45820807254448	41.0	39.0	41.0	36.0	41.0
26	39.28394362032428	41.0	39.0	41.0	35.0	41.0
27	39.163767187423	41.0	39.0	41.0	35.0	41.0
28	39.033931299590954	41.0	39.0	41.0	35.0	41.0
29	38.90303582869252	40.0	38.0	41.0	35.0	41.0
30	38.66928687595486	40.0	38.0	41.0	35.0	41.0
31	38.45786309201124	40.0	37.0	41.0	35.0	41.0
32	38.26107633926371	40.0	37.0	41.0	35.0	41.0
33	38.038317480656445	40.0	36.0	41.0	35.0	41.0
34	37.89276033709526	40.0	35.0	41.0	35.0	41.0
35	37.68678231728352	40.0	35.0	41.0	34.0	41.0
36	37.48767926666995	40.0	35.0	41.0	34.0	41.0
37	37.25373318219901	40.0	35.0	41.0	33.0	41.0
38	37.03903208318959	39.0	35.0	41.0	33.0	41.0
39	36.73963826326943	39.0	35.0	41.0	33.0	41.0
40	36.471070918141045	39.0	35.0	41.0	32.0	41.0
41	36.170962495687746	39.0	35.0	41.0	31.0	41.0
42	35.8130205509832	39.0	35.0	41.0	30.0	41.0
43	34.84796215070721	37.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	4.0
21	14.0
22	18.0
23	30.0
24	35.0
25	65.0
26	97.0
27	160.0
28	246.0
29	331.0
30	423.0
31	515.0
32	594.0
33	811.0
34	1107.0
35	1509.0
36	2493.0
37	6114.0
38	8589.0
39	17423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.20274998767926	15.635010595830664	13.607017889704794	24.555221526785274
2	20.787541274456657	20.735794194470454	31.343945591641614	27.132718939431278
3	21.82001872751466	22.201961460746144	27.972992952540537	28.00502685919866
4	15.105219062638609	16.805480262185206	29.542654378788626	38.54664629638756
5	16.487605342269973	36.351091616973044	28.27854713912572	18.882755901631263
6	31.90330688482578	29.954166872012223	18.13858360849638	20.003942634665613
7	31.558326351584448	30.402641565225963	17.342664235375292	20.6963678478143
8	34.24671036420088	27.76354048592972	16.87940466216549	21.11034448770391
9	30.47903011187226	12.323197476713814	17.39194716869548	39.80582524271845
10	22.28327830072446	25.06037159331723	23.51535163372924	29.140998472229068
11	36.279631363658766	23.769158740328226	15.975062835739983	23.97614706027303
12	24.813956926716276	27.37913360603223	23.611453353703613	24.195456113547877
13	35.33339904391109	17.90202552855946	23.318219900448476	23.44635552708097
14	25.163865753289638	20.348923167906953	23.397072593760782	31.09013848504263
15	32.09797447144054	23.017594007195306	19.895520181361196	24.988911340002957
16	26.346656152974223	23.95396974027894	23.79626435365433	25.9031097530925
17	22.647972007293873	27.49002020600266	24.003252673599132	25.85875511310433
18	24.321127593514365	21.694347247548173	26.965156966142622	27.019368192794836
19	24.870632300034497	25.920358779754572	27.684687792617417	21.524321127593517
20	26.243161993001824	21.90133556749298	28.719629392341435	23.135873047163766
21	28.54713912572076	20.957567394411317	26.73845547286974	23.756838006998176
22	27.628012419299196	21.88901483416293	24.914986940022672	25.567985806515203
23	24.996303780000986	23.434034793750925	25.50884628653097	26.060815139717114
24	24.29648612685427	23.78640776699029	27.60090680597309	24.316199300182348
25	24.010645113597164	23.67059287368784	26.011532206396925	26.307229806318073
26	26.08545660637721	22.817998127248533	25.824257059780198	25.272288206594055
27	25.013552806663053	23.0151298605293	25.462027499876793	26.509289832930854
28	23.759302153664187	23.658272140357795	26.94544379281455	25.63698191316347
29	23.692770193681927	23.936720713616875	28.423931792420287	23.946577300280914
30	23.28865014045636	24.50347444679907	29.471194125474348	22.73668128727022
31	23.276329407126312	25.363461633236412	26.72120644620768	24.6390025134296
32	22.850032033906658	25.30678625991819	28.638312552363114	23.204869153812034
33	23.313291607116454	23.79133606032231	28.921689418954216	23.973682913607018
34	23.889901926962693	24.24966734020009	26.546252032920997	25.31417869991622
35	22.995416687201224	25.452170913212758	26.893696712828348	24.658715686757677
36	21.576068207579716	24.85584742003844	27.97052880587453	25.597555566507317
37	21.686954807550148	24.57986299344537	28.512641072396626	25.220541126607856
38	21.563747474249666	22.85249618057267	29.80385392538564	25.779902419792027
39	22.041791927455524	22.408949780690946	30.678625991819032	24.870632300034497
40	21.89147898082894	22.174855847420037	29.16317579222315	26.770489379527866
41	19.033068848257848	22.761322753930312	32.065940564782416	26.139667833029424
42	19.25977034153073	22.349810260706718	31.26509289832931	27.125326499433243
43	18.205115568478636	23.835690700310483	32.341924991375485	25.617268739835396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	5.5
2	9.0
3	34.0
4	59.0
5	59.0
6	83.0
7	107.0
8	110.5
9	114.0
10	188.5
11	263.0
12	263.0
13	456.5
14	650.0
15	1045.5
16	1441.0
17	1280.5
18	1120.0
19	1120.0
20	1230.0
21	1340.0
22	910.5
23	481.0
24	436.5
25	392.0
26	392.0
27	351.5
28	311.0
29	312.0
30	313.0
31	344.5
32	376.0
33	376.0
34	380.5
35	385.0
36	405.5
37	426.0
38	554.5
39	683.0
40	683.0
41	788.5
42	894.0
43	1154.0
44	1414.0
45	1746.5
46	2079.0
47	2079.0
48	2701.0
49	3323.0
50	3351.0
51	3379.0
52	2933.0
53	2487.0
54	2487.0
55	2372.5
56	2258.0
57	2322.5
58	2387.0
59	2360.5
60	2334.0
61	2334.0
62	2315.5
63	2297.0
64	2260.0
65	2223.0
66	2121.0
67	2019.0
68	2019.0
69	1864.0
70	1709.0
71	1516.0
72	1323.0
73	1118.5
74	914.0
75	914.0
76	717.0
77	520.0
78	412.5
79	305.0
80	220.5
81	136.0
82	136.0
83	99.0
84	62.0
85	44.5
86	27.0
87	20.5
88	14.0
89	14.0
90	8.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	40582.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.95283623281257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.39075858977233	71.86683751416885
2	3.6632617118703767	5.637967571829875
3	1.0374971981171348	2.395150559361293
4	0.5027378398283646	1.5474841062540041
5	0.3426302475263377	1.318318466315115
6	0.1729161996861891	0.7983835197870977
7	0.17932050337827019	0.965945493075748
8	0.09286240353517564	0.5716820265142181
9	0.10887316276537834	0.7540288797989256
>10	0.445099106599635	6.283573998324381
>50	0.04803227769060809	2.496180572667685
>100	0.006404303692081078	0.830417426445222
>500	0.009606455538121618	4.534029865457592
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	707	1.7421516928687597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	611	1.5055936129318417	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	522	1.2862845596569907	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.4435463998817209	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	157	0.38687102656350103	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	94	0.23162978660489875	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.1995958799467744	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	79	0.1946675866147553	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	79	0.1946675866147553	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.1946675866147553	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	67	0.16509782662264058	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	67	0.16509782662264058	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.16263367995663103	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	63	0.15524123995860234	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	63	0.15524123995860234	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	62	0.15277709329259279	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	55	0.13552806663052586	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.13306391996451628	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	53	0.13059977329850672	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.12567147996648761	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	50	0.12320733330047805	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	47	0.11581489330244937	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.11088659997043022	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	43	0.1059583066384111	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	41	0.101030013306392	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.101030013306392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.002464146666009561	0.0
16	0.0	0.0	0.0	0.004928293332019122	0.0
17	0.0	0.0	0.0	0.007392439998028683	0.0
18	0.0	0.0	0.0	0.009856586664038243	0.0
19	0.0	0.0	0.0	0.009856586664038243	0.0
20	0.0	0.0	0.0	0.014784879996057366	0.0
21	0.0	0.0	0.0	0.019713173328076487	0.0
22	0.0	0.0	0.0	0.022177319994086047	0.0
23	0.0	0.0	0.0	0.03203390665812429	0.0
24	0.0	0.0	0.0	0.05421122665221034	0.0
25	0.0	0.0	0.0	0.05913951998422946	0.0
26	0.0	0.0	0.0	0.08624513331033463	0.0
27	0.0	0.0	0.0	0.17988270661869796	0.0
28	0.0	0.0	0.0	0.4115124932235967	0.0
29	0.0	0.0	0.0	0.7762061997930116	0.0
30	0.0	0.0	0.0	1.1729338130205509	0.0
31	0.0	0.0	0.0	2.0181361194618304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCGC	115	1.2187229E-10	20.913044	21
GCCTTAT	115	1.2187229E-10	20.913044	26
CCTTATT	120	2.1645974E-10	20.041668	27
TCGCCTT	115	2.8030627E-9	19.304348	24
CTTATAC	115	2.8030627E-9	19.304348	37
TTCCTCG	125	3.7289283E-10	19.24	20
CTTATTG	145	7.2759576E-12	19.13793	28
GGTGATT	155	0.0	19.096775	15
GTGATTC	155	0.0	19.096775	16
CGCCTTA	120	4.7548383E-9	18.5	25
CCTCGCC	120	4.7548383E-9	18.5	22
TTGATAT	120	4.7548383E-9	18.5	32
GATTCCT	160	1.8189894E-12	18.5	18
TGGGCAG	160	1.8189894E-12	18.5	6
GGGCAGT	165	1.8189894E-12	17.939394	7
TGATTCC	165	1.8189894E-12	17.939394	17
GGCAGTC	165	1.8189894E-12	17.939394	8
GTCGGTG	165	1.8189894E-12	17.939394	12
ATTCCTC	155	1.8189894E-11	17.903227	19
GATATGC	125	7.887138E-9	17.76	34
>>END_MODULE