FastQCFastQC Report
Fri 10 Feb 2017
ERR1631317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335973
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77902.3186387001336417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59371.7671062853264994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56011.6670982489664348No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA29490.8777491048387817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22390.6664225994350736No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT20680.6155256523589693No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT20560.6119539367746814No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT18460.5494489140496409No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA17910.5330785509549875No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT17340.5161129019296193No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC14830.44140451762492816No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC14720.4381304450059975No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA14240.4238435826688454No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA12320.36669613332023704No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT10870.3235379033434234No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT9570.28484431784696984No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT9370.27889145853982317No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9250.27531974295553513No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT8790.2616281665490977No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA8530.253889449449807No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA8160.2428766597315856No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA7970.2372214433897962No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT7550.22472043884478812No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA7310.21757700767621205No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT7300.21727936471085474No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA6950.206861860923348No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC6810.2026948594083453No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.2018019305122733No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6510.19376557044762527No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT6510.19376557044762527No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA6430.19138442672476658No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA6330.1884079970711932No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT6250.18602685334833455No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.18424099555619053No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6130.1824551377640465No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT6070.18066927997190252No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG6000.17858577921440114No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT5980.1779904932836865No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC5910.17590699252618514No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA5910.17590699252618514No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT5830.17352584880332644No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT5780.1720376339765398No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT5730.1705494191497531No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5410.16102484425831837No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT5330.1586437005354597No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC5150.15328612715902767No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT4990.1485238397133103No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT4960.14763091081723828No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC4900.1458450530250943No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTA4730.1407851226140196No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT4710.14018983668330492No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT4680.1392969077872329No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT4640.13810633592580354No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4570.13602283516830221No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA4520.13453462034151556No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.13364169144544352No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT4410.13126054772258486No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC4380.13036761882651285No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT4290.12768883213829682No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT4250.12649826027686747No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT4230.1259029743461528No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG4140.12322418765793679No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT4100.12203361579650747No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA4080.1214383298657928No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT4020.11965247207364876No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA3690.10983025421685672No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.10953261125149938No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTT3680.10953261125149938No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT3630.1080443964247127No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA3550.10566325270185402No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.10477032380578201No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT3440.10238918008292333No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT3410.10149625118685132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA200.001840082837.025
GACCGTT251.2317665E-437.07
TCTGCAA303.594106E-430.83333210
AAACACG303.594106E-430.83333215
ACCGTTA303.594106E-430.8333328
TGGACGT250.00549087629.626
TAAGTTC502.7110946E-729.630
GGACGTA250.00549087629.627
GACGGGG250.00549087629.61
GGACCAC250.00549087629.66
GTAAGTT502.7110946E-729.629
ATGCCCC3400.028.29411937
GCTTCTG405.927814E-527.7527
GGACCGT405.927814E-527.756
GTCGGGT604.303729E-827.74999824
TATGCTG754.6020432E-1027.13333336
GGTAAGT556.2395884E-726.9090928
TCGGGTA556.2395884E-726.9090925
CAAACAC358.85628E-426.42857214
ATATCTG358.85628E-426.42857235
Produced by FastQC (version 0.11.5)