##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335973
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00413426078882	34.0	33.0	34.0	31.0	34.0
2	33.093159271727195	34.0	33.0	34.0	31.0	34.0
3	33.05231075116155	34.0	33.0	34.0	31.0	34.0
4	36.38596851532712	37.0	37.0	37.0	35.0	37.0
5	36.43158229976814	37.0	37.0	37.0	35.0	37.0
6	36.53067657222456	37.0	37.0	37.0	35.0	37.0
7	36.53837064287904	37.0	37.0	37.0	35.0	37.0
8	36.573331785589914	37.0	37.0	37.0	35.0	37.0
9	38.386834656356314	39.0	39.0	39.0	37.0	39.0
10	38.375673045155416	39.0	39.0	39.0	37.0	39.0
11	38.41099433585437	39.0	39.0	39.0	37.0	39.0
12	38.40852985210121	39.0	39.0	39.0	37.0	39.0
13	38.40560104532209	39.0	39.0	39.0	37.0	39.0
14	39.80283237045834	41.0	40.0	41.0	38.0	41.0
15	39.74701240873522	41.0	39.0	41.0	38.0	41.0
16	39.62734803094296	41.0	39.0	41.0	37.0	41.0
17	39.621118363678036	41.0	39.0	41.0	37.0	41.0
18	39.83134359010992	41.0	40.0	41.0	38.0	41.0
19	39.89270566384799	41.0	40.0	41.0	38.0	41.0
20	39.96211302693966	41.0	40.0	41.0	38.0	41.0
21	39.966437779226304	41.0	40.0	41.0	38.0	41.0
22	39.889363133347025	41.0	40.0	41.0	38.0	41.0
23	39.83339435014123	41.0	40.0	41.0	38.0	41.0
24	39.789983123643864	41.0	40.0	41.0	37.0	41.0
25	39.76073672586785	41.0	40.0	41.0	37.0	41.0
26	39.62198450470722	41.0	40.0	41.0	37.0	41.0
27	39.50804380113878	41.0	40.0	41.0	35.0	41.0
28	39.35975212293845	41.0	39.0	41.0	35.0	41.0
29	39.183499864572454	41.0	39.0	41.0	35.0	41.0
30	38.963973295473146	40.0	38.0	41.0	35.0	41.0
31	38.70931592717272	40.0	38.0	41.0	35.0	41.0
32	38.51430323270025	40.0	37.0	41.0	35.0	41.0
33	38.364270343152576	40.0	37.0	41.0	35.0	41.0
34	38.229932167168194	40.0	36.0	41.0	35.0	41.0
35	38.08962029686909	40.0	36.0	41.0	35.0	41.0
36	37.943191863631895	40.0	35.0	41.0	35.0	41.0
37	37.78027401011391	40.0	35.0	41.0	35.0	41.0
38	37.59051173755034	40.0	35.0	41.0	35.0	41.0
39	37.325237444675615	40.0	35.0	41.0	34.0	41.0
40	37.106428195122824	40.0	35.0	41.0	33.0	41.0
41	36.78184556497099	40.0	35.0	41.0	33.0	41.0
42	36.472755251166014	40.0	35.0	41.0	31.0	41.0
43	35.60408723320029	39.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	4.0
14	1.0
15	4.0
16	6.0
17	5.0
18	6.0
19	6.0
20	28.0
21	65.0
22	91.0
23	167.0
24	225.0
25	416.0
26	630.0
27	1074.0
28	1623.0
29	2347.0
30	3036.0
31	3632.0
32	4181.0
33	5552.0
34	7641.0
35	10780.0
36	17007.0
37	47170.0
38	60341.0
39	169924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87703773815158	12.413199870227668	18.855979498352546	24.85378289326821
2	19.622112491182328	20.379316195051388	36.44370232131749	23.5548689924488
3	17.73059144633646	24.131105773380597	32.12490289398255	26.013399886300387
4	11.8241644417855	20.746012328371624	32.97616177490453	34.45366145493834
5	12.507255047280585	40.68809100731309	30.292017513312082	16.512636432094247
6	24.408806660058993	31.769814836311248	23.837034523607553	19.984343980022203
7	29.360097388778268	33.436020156381616	17.225193691159706	19.978688763680417
8	36.231482887017705	28.19988510981537	16.68288820827864	18.88574379488828
9	33.15087819556929	12.534042914162747	15.407785744687816	38.907293145580155
10	21.44815208364958	26.821202894280194	20.31026302708849	31.42038199498174
11	30.968559973569302	28.96452988781837	13.86926925675575	26.197640881856575
12	25.465439187077532	32.54398418920568	23.4474198819548	18.543156741761987
13	35.70614305316201	18.69019236664851	25.876186479270658	19.727478100918823
14	23.796555080318953	21.965455557440627	26.00625645513181	28.231732907108608
15	33.76848734868575	23.251273167784316	20.741845326856623	22.238394156673305
16	21.919023254844884	26.921508573605617	27.24891583549869	23.910552336050813
17	15.72120378720909	32.97764998973132	27.46292112759061	23.83822509546898
18	22.320841258077287	22.08689388730642	30.737291389486654	24.85497346512964
19	20.96864926645891	31.809698993669134	31.2950743065663	15.926577433305653
20	25.29846148351207	21.889556601274506	34.26376524304036	18.548216672173062
21	31.116786170317255	19.24440356814387	30.427742705515026	19.21106755602385
22	27.759373521086516	21.266887517746962	25.270780687733836	25.702958273432685
23	22.184520779943625	25.284174621174916	25.854756185764927	26.676548413116528
24	20.27990344462204	25.412756382209285	33.766106204962895	20.541233968205777
25	19.25214228524316	24.586499510377323	28.5945596818792	27.566798522500317
26	26.298839490078073	21.760379554309424	27.53673658299923	24.404044372613278
27	23.070306244847057	22.35923720060838	26.538442077190727	28.032014477353833
28	19.849214073749973	23.115250332616014	32.14663083045364	24.888904763180374
29	20.707318742875174	23.990915936697295	34.76916299821712	20.532602322210415
30	19.821533277971742	25.007366663392595	36.77140722617591	18.399692832459753
31	20.076315656317618	27.97308117021308	30.051521997303354	21.89908117616594
32	18.22140469621071	26.958713944275285	36.71753384944624	18.102347510067773
33	20.345980182931367	23.789114006185024	36.62020459977439	19.244701211109223
34	22.123503972045373	25.89285448533067	28.45139341554232	23.53224812708164
35	19.383998118896457	27.706095430287554	29.722328877618143	23.187577573197846
36	14.968167084855033	26.18662809213836	32.781800918526194	26.06340390448042
37	16.873379706107336	26.02649617677611	33.955109487964805	23.145014629151746
38	17.93775095022517	20.336455608039934	36.007357734103635	25.718435707631265
39	18.382429540469026	20.19567048542591	38.862349057811194	22.55955091629387
40	17.5921874674453	19.983153408160774	34.30007768481396	28.124581439579966
41	12.42748673256482	22.64973673479714	39.84159441383683	25.081182118801216
42	12.809660300083639	21.37909891568668	36.872308191432055	28.938932592797634
43	10.750268622776234	25.920535281108897	40.325264232542494	23.003931863572372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	34.5
2	60.0
3	262.0
4	464.0
5	464.0
6	731.0
7	998.0
8	1073.5
9	1149.0
10	1853.0
11	2557.0
12	2557.0
13	4715.0
14	6873.0
15	10834.5
16	14796.0
17	12980.0
18	11164.0
19	11164.0
20	11829.0
21	12494.0
22	8443.5
23	4393.0
24	3733.0
25	3073.0
26	3073.0
27	2837.5
28	2602.0
29	2633.5
30	2665.0
31	2697.0
32	2729.0
33	2729.0
34	2650.0
35	2571.0
36	2637.5
37	2704.0
38	3584.0
39	4464.0
40	4464.0
41	6769.5
42	9075.0
43	13574.0
44	18073.0
45	25469.0
46	32865.0
47	32865.0
48	44844.0
49	56823.0
50	51425.0
51	46027.0
52	34901.0
53	23775.0
54	23775.0
55	17604.0
56	11433.0
57	10671.0
58	9909.0
59	9635.5
60	9362.0
61	9362.0
62	9122.5
63	8883.0
64	8701.5
65	8520.0
66	7971.5
67	7423.0
68	7423.0
69	6822.5
70	6222.0
71	5453.5
72	4685.0
73	3983.5
74	3282.0
75	3282.0
76	2636.0
77	1990.0
78	1527.5
79	1065.0
80	787.0
81	509.0
82	509.0
83	351.5
84	194.0
85	130.0
86	66.0
87	42.0
88	18.0
89	18.0
90	13.0
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	335973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.75573743197042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.01507469378312	38.07408760924468
2	6.507707625526571	5.694990922931445
3	2.0268888263001528	2.660640458621525
4	0.9874529551462209	1.7282692892720521
5	0.6119746419708997	1.3388700874551391
6	0.4520085330030942	1.1866780012256115
7	0.3150400549917937	0.9649366948742114
8	0.2194719937311546	0.7682527145097164
9	0.19558425022220902	0.77021397886966
>10	1.3478492100528026	11.445754995397575
>50	0.1526373168172614	4.702263305708818
>100	0.13696473517977473	12.212586709900016
>500	0.02112391438096028	6.314706394750304
>1k	0.008176999115210432	6.374934483505182
>5k	0.002044249778802608	5.76281435373407
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7790	2.3186387001336417	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5937	1.7671062853264994	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5601	1.6670982489664348	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2949	0.8777491048387817	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2239	0.6664225994350736	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	2068	0.6155256523589693	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	2056	0.6119539367746814	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	1846	0.5494489140496409	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1791	0.5330785509549875	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	1734	0.5161129019296193	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	1483	0.44140451762492816	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	1472	0.4381304450059975	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	1424	0.4238435826688454	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	1232	0.36669613332023704	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	1087	0.3235379033434234	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	957	0.28484431784696984	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	937	0.27889145853982317	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	925	0.27531974295553513	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	879	0.2616281665490977	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	853	0.253889449449807	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	816	0.2428766597315856	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	797	0.2372214433897962	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	755	0.22472043884478812	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	731	0.21757700767621205	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	730	0.21727936471085474	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	695	0.206861860923348	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	681	0.2026948594083453	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	678	0.2018019305122733	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	651	0.19376557044762527	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	651	0.19376557044762527	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	643	0.19138442672476658	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	633	0.1884079970711932	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	625	0.18602685334833455	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.18424099555619053	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	613	0.1824551377640465	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	607	0.18066927997190252	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	600	0.17858577921440114	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	598	0.1779904932836865	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	591	0.17590699252618514	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	591	0.17590699252618514	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	583	0.17352584880332644	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	578	0.1720376339765398	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	573	0.1705494191497531	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	541	0.16102484425831837	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	533	0.1586437005354597	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	515	0.15328612715902767	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	499	0.1485238397133103	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	496	0.14763091081723828	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	490	0.1458450530250943	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTA	473	0.1407851226140196	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT	471	0.14018983668330492	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	468	0.1392969077872329	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT	464	0.13810633592580354	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	457	0.13602283516830221	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA	452	0.13453462034151556	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.13364169144544352	No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	441	0.13126054772258486	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC	438	0.13036761882651285	No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT	429	0.12768883213829682	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT	425	0.12649826027686747	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	423	0.1259029743461528	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	414	0.12322418765793679	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	410	0.12203361579650747	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA	408	0.1214383298657928	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	402	0.11965247207364876	No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	369	0.10983025421685672	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.10953261125149938	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTT	368	0.10953261125149938	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT	363	0.1080443964247127	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA	355	0.10566325270185402	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	352	0.10477032380578201	No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT	344	0.10238918008292333	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	341	0.10149625118685132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.976429653573353E-4	0.0
11	0.0	0.0	0.0	0.0014882148267866763	0.0
12	0.0	0.0	0.0	0.0029764296535733526	0.0
13	0.0	0.0	0.0	0.0029764296535733526	0.0
14	0.0	0.0	0.0	0.005952859307146705	0.0
15	0.0	0.0	0.0	0.010119860822149399	0.0
16	0.0	0.0	0.0	0.025299652055373496	0.0
17	0.0	0.0	0.0	0.04107472921931227	0.0
18	0.0	0.0	0.0	0.046134659630386966	0.0
19	0.0	0.0	0.0	0.0565521634178937	0.0
20	0.0	0.0	0.0	0.06637438127468577	0.0
21	0.0	0.0	0.0	0.08006595768112318	0.0
22	0.0	0.0	0.0	0.10447268084042467	0.0
23	0.0	0.0	0.0	0.15566727088188634	2.976429653573353E-4
24	0.0	0.0	0.0	0.22352986698335878	2.976429653573353E-4
25	0.0	0.0	0.0	0.24585308938515893	2.976429653573353E-4
26	0.0	0.0	0.0	0.2851419608123272	2.976429653573353E-4
27	0.0	0.0	0.0	0.3830664964148905	2.976429653573353E-4
28	0.0	0.0	0.0	0.6673155283311456	2.976429653573353E-4
29	0.0	0.0	0.0	1.303378545299771	2.976429653573353E-4
30	0.0	0.0	0.0	2.2668488241614653	2.976429653573353E-4
31	0.0	0.0	0.0	4.225934822143446	2.976429653573353E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTTA	20	0.0018400828	37.0	25
GACCGTT	25	1.2317665E-4	37.0	7
TCTGCAA	30	3.594106E-4	30.833332	10
AAACACG	30	3.594106E-4	30.833332	15
ACCGTTA	30	3.594106E-4	30.833332	8
TGGACGT	25	0.005490876	29.6	26
TAAGTTC	50	2.7110946E-7	29.6	30
GGACGTA	25	0.005490876	29.6	27
GACGGGG	25	0.005490876	29.6	1
GGACCAC	25	0.005490876	29.6	6
GTAAGTT	50	2.7110946E-7	29.6	29
ATGCCCC	340	0.0	28.294119	37
GCTTCTG	40	5.927814E-5	27.75	27
GGACCGT	40	5.927814E-5	27.75	6
GTCGGGT	60	4.303729E-8	27.749998	24
TATGCTG	75	4.6020432E-10	27.133333	36
GGTAAGT	55	6.2395884E-7	26.90909	28
TCGGGTA	55	6.2395884E-7	26.90909	25
CAAACAC	35	8.85628E-4	26.428572	14
ATATCTG	35	8.85628E-4	26.428572	35
>>END_MODULE