FastQCFastQC Report
Fri 10 Feb 2017
ERR1631316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481445
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT35770.144149880412421No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33810.13625125682817874No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT32010.12899741884265015No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT31780.12807053954449926No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC29660.11952713036154337No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA28250.11384495727287931No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26440.1065508201874311No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC25380.10227911559595317No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG25200.10155373179740029No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC24870.10022386150005339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGTA6350.023.8897658
TCTAGAC8350.022.5988033
CTAGACA8600.021.941864
ACTGATC4650.021.0860218
GTCTAGA6450.020.9379841
GTTCTAG9650.020.8963741
GGTATCA17400.020.6264381
GACATGT7500.020.2266657
TAATCCG555.145404E-420.1818189
TAGAACT7050.019.418444
GTATTTT7850.018.85350412
TTAACGG500.007037122718.535
GTATATC500.007037122718.51
CTAGAAC7450.018.1275163
TCTAGTT10350.018.0531428
TATTTTC8200.018.04878213
ATACACA4850.017.92783537
AGTTTTT10550.017.88625531
CTCTAGT10450.017.88038327
TCGTTTA7050.017.84397130
Produced by FastQC (version 0.11.5)