##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631316.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2481445
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.919174513237245	34.0	31.0	34.0	31.0	34.0
2	33.07240619880755	34.0	33.0	34.0	31.0	34.0
3	33.16585013973713	34.0	33.0	34.0	31.0	34.0
4	36.45171664090883	37.0	37.0	37.0	35.0	37.0
5	36.39937294600525	37.0	37.0	37.0	35.0	37.0
6	36.441103066963	37.0	37.0	37.0	35.0	37.0
7	36.45088446449549	37.0	37.0	37.0	35.0	37.0
8	36.440538476573124	37.0	37.0	37.0	35.0	37.0
9	38.255709878719856	39.0	39.0	39.0	37.0	39.0
10	38.19100322594295	39.0	38.0	39.0	37.0	39.0
11	38.25823622929382	39.0	39.0	39.0	37.0	39.0
12	38.20903949110296	39.0	38.0	39.0	37.0	39.0
13	38.239537043940125	39.0	38.0	39.0	37.0	39.0
14	39.73621942053924	41.0	40.0	41.0	38.0	41.0
15	39.716853688072874	41.0	40.0	41.0	38.0	41.0
16	39.6979449473996	41.0	40.0	41.0	38.0	41.0
17	39.67946700410447	41.0	40.0	41.0	38.0	41.0
18	39.652959868141345	41.0	40.0	41.0	37.0	41.0
19	39.652103915259055	41.0	40.0	41.0	37.0	41.0
20	39.62173330458664	41.0	40.0	41.0	37.0	41.0
21	39.5837078798845	41.0	40.0	41.0	37.0	41.0
22	39.53504188083959	41.0	39.0	41.0	37.0	41.0
23	39.47362524658012	41.0	39.0	41.0	37.0	41.0
24	39.44209805174001	41.0	39.0	41.0	37.0	41.0
25	39.431937036686286	41.0	39.0	41.0	37.0	41.0
26	39.34418332866535	41.0	39.0	41.0	36.0	41.0
27	39.286428270624576	40.0	39.0	41.0	36.0	41.0
28	39.210908563357236	40.0	39.0	41.0	36.0	41.0
29	39.17072310689941	40.0	39.0	41.0	36.0	41.0
30	39.10688731767176	40.0	39.0	41.0	35.0	41.0
31	39.07523277767591	40.0	39.0	41.0	35.0	41.0
32	38.988231050859476	40.0	39.0	41.0	35.0	41.0
33	38.91333557664989	40.0	38.0	41.0	35.0	41.0
34	38.86183413293464	40.0	38.0	41.0	35.0	41.0
35	38.76756285148371	40.0	38.0	41.0	35.0	41.0
36	38.703094366387326	40.0	38.0	41.0	35.0	41.0
37	38.65529963388268	40.0	38.0	41.0	35.0	41.0
38	38.57811234986067	40.0	38.0	41.0	35.0	41.0
39	38.51069316466817	40.0	38.0	41.0	35.0	41.0
40	38.42002502574105	40.0	38.0	41.0	35.0	41.0
41	38.33889125086391	40.0	38.0	41.0	34.0	41.0
42	38.20137460229826	40.0	37.0	41.0	34.0	41.0
43	37.33164184577937	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	2.0
13	0.0
14	3.0
15	4.0
16	14.0
17	22.0
18	32.0
19	92.0
20	166.0
21	309.0
22	591.0
23	930.0
24	1543.0
25	2376.0
26	3476.0
27	4902.0
28	7069.0
29	9696.0
30	12924.0
31	17462.0
32	23027.0
33	32085.0
34	48042.0
35	73278.0
36	125214.0
37	247326.0
38	636312.0
39	1234544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78056132616278	16.79299762839797	10.858834267936626	27.567606777502622
2	20.387757939426425	19.43339465513038	32.03826802528366	28.14057938015954
3	20.976971079350943	19.95619487838739	28.018513406503065	31.048320635758603
4	15.314302755047965	14.068818772932707	31.816743873025594	38.80013459899373
5	17.360328357066145	33.62286893322238	31.55213998295348	17.464662726758
6	37.87204632784527	31.930709727598234	14.551601989969553	15.645641954586944
7	32.40668239674867	28.00485201163032	19.132360378730944	20.45610521289007
8	29.1671183524116	30.08114223768812	19.125670728144286	21.626068681755992
9	27.89382799135181	13.060293498344713	18.19907352369285	40.84680498661062
10	18.711516878270523	24.231889080757384	30.118056213214476	26.93853782775762
11	39.61482120296843	19.5631577568715	19.325070674546485	21.496950365613586
12	24.81626632869155	23.40728889820246	25.85505622731916	25.921388545786826
13	31.96992075182001	16.771921199140017	23.447587998122064	27.810570050917917
14	24.829806826264537	18.704142142985237	21.702072784204365	34.76397824654586
15	27.462950015011415	25.29586591683475	19.750669468797412	27.490514599356423
16	27.95927372962125	23.433846005049478	21.366582777373665	27.240297487955605
17	26.75150970503074	23.93520710714926	22.35290324790596	26.96037993991404
18	27.66251115781329	21.631912051244335	23.666089717886152	27.039487073056222
19	27.08228471717084	22.689199236735046	22.875098984664177	27.353417061429937
20	27.15115587893344	22.175667806459543	23.063174883988964	27.61000143061805
21	28.809020550526004	22.44381801732458	23.05741211270046	25.68974931944895
22	28.636137411870905	21.994926343320124	22.013705723882655	27.355230520926316
23	26.78705351115983	22.120216244970166	23.167670450080497	27.925059793789508
24	26.875590633683196	22.922853418068907	22.569188517174467	27.63236743107343
25	27.466818728603698	22.209680246791688	22.72264748966832	27.6008535349363
26	27.550399061836956	22.92063696757333	22.464491455583342	27.064472515006376
27	27.867875371003585	21.872860369663645	22.74388511532595	27.51537914400682
28	26.487671497857097	22.284112684343196	23.80044691701811	27.4277689007816
29	26.319906344891784	22.63507754554302	23.296023083324435	27.748993026240758
30	25.624988665878146	23.162552464390707	24.40166918871867	26.810789681012476
31	27.668032134502273	22.72123702117113	22.254815238701646	27.355915605624947
32	26.538166270056358	22.35471670740234	22.896699302221084	28.21041772032022
33	26.36637120709909	21.9439076828219	23.461773281293762	28.227947828785243
34	27.44505721464711	21.83356874724203	23.47922279155895	27.24215124655191
35	26.823967486686186	22.053561533703146	24.35415654991346	26.768314429697217
36	25.779414816770068	22.25836155949457	24.552790813417182	27.40943281031818
37	26.77701903527985	21.705417609497694	24.618357448986377	26.899205906236084
38	25.781429771766046	20.892101174920256	25.765108636298606	27.561360417015084
39	26.514389801103793	20.934334631636002	25.412128820102804	27.1391467471574
40	25.598149465331694	22.125535726159555	25.376585013973713	26.899729794535038
41	25.337978476250733	21.479339658948717	26.60393440112515	26.578747463675402
42	25.90732415991489	21.666649875375033	26.21194505620717	26.214080908502908
43	24.245510176530207	21.009089461986864	26.307212128417113	28.438188233065816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	231.0
1	214.0
2	197.0
3	356.5
4	516.0
5	516.0
6	611.0
7	706.0
8	609.5
9	513.0
10	753.0
11	993.0
12	993.0
13	1691.5
14	2390.0
15	3340.0
16	4290.0
17	4116.5
18	3943.0
19	3943.0
20	4715.5
21	5488.0
22	5008.5
23	4529.0
24	5369.5
25	6210.0
26	6210.0
27	7605.5
28	9001.0
29	11756.5
30	14512.0
31	18388.0
32	22264.0
33	22264.0
34	30826.5
35	39389.0
36	51000.5
37	62612.0
38	73875.0
39	85138.0
40	85138.0
41	94790.5
42	104443.0
43	112510.0
44	120577.0
45	137612.5
46	154648.0
47	154648.0
48	167413.5
49	180179.0
50	188831.5
51	197484.0
52	205055.0
53	212626.0
54	212626.0
55	213625.5
56	214625.0
57	211175.0
58	207725.0
59	203053.0
60	198381.0
61	198381.0
62	186944.5
63	175508.0
64	160966.0
65	146424.0
66	130382.5
67	114341.0
68	114341.0
69	93239.0
70	72137.0
71	61904.5
72	51672.0
73	38215.5
74	24759.0
75	24759.0
76	18743.5
77	12728.0
78	10398.5
79	8069.0
80	7566.0
81	7063.0
82	7063.0
83	5898.0
84	4733.0
85	4689.5
86	4646.0
87	4218.0
88	3790.0
89	3790.0
90	2732.5
91	1675.0
92	940.0
93	205.0
94	134.0
95	63.0
96	63.0
97	40.0
98	17.0
99	11.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2481445.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.408380855954054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32661692579978	25.85745738987753
2	8.010166656722586	5.031727301480142
3	2.7329139960050353	2.575092108992804
4	1.4684006564952925	1.8448034827334845
5	0.9376253183527679	1.4724646549504452
6	0.6374368329875336	1.201251529325138
7	0.44852019693768125	0.9861105216904347
8	0.3602476333895903	0.9051835897565091
9	0.2925015219415371	0.8268299281877398
>10	2.0509757650077787	13.013990913880283
>50	0.3390588929343909	7.552277274534683
>100	0.33623811964153705	21.63538848251108
>500	0.0439907580353695	9.466517603022952
>1k	0.015306725749149035	7.63090521905679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3577	0.144149880412421	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3381	0.13625125682817874	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	3201	0.12899741884265015	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3178	0.12807053954449926	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2966	0.11952713036154337	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	2825	0.11384495727287931	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2644	0.1065508201874311	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2538	0.10227911559595317	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	2520	0.10155373179740029	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2487	0.10022386150005339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.0299099919603294E-5	0.0
4	0.0	0.0	0.0	4.0299099919603294E-5	0.0
5	0.0	0.0	0.0	4.0299099919603294E-5	0.0
6	0.0	0.0	0.0	4.0299099919603294E-5	0.0
7	0.0	0.0	0.0	4.0299099919603294E-5	0.0
8	0.0	0.0	0.0	4.0299099919603294E-5	0.0
9	0.0	0.0	0.0	4.0299099919603294E-5	0.0
10	0.0	0.0	0.0	4.0299099919603294E-5	0.0
11	0.0	0.0	0.0	4.0299099919603294E-5	0.0
12	0.0	0.0	0.0	1.2089729975880989E-4	0.0
13	0.0	0.0	0.0	1.2089729975880989E-4	0.0
14	0.0	0.0	0.0	1.2089729975880989E-4	0.0
15	0.0	0.0	0.0	2.8209369943722306E-4	0.0
16	0.0	0.0	0.0	6.85084698633256E-4	0.0
17	0.0	0.0	0.0	0.0014104684971861154	0.0
18	0.0	0.0	0.0	0.001611963996784132	0.0
19	0.0	0.0	0.0	0.0018134594963821484	0.0
20	0.0	0.0	0.0	0.002337347795336991	0.0
21	0.0	0.0	0.0	0.0031030306938094536	0.0
22	0.0	0.0	0.0	0.004352302791317156	0.0
23	0.0	4.0299099919603294E-5	0.0	0.0063672577872973205	0.0
24	0.0	4.0299099919603294E-5	0.0	0.00906729748191074	0.0
25	0.0	4.0299099919603294E-5	0.0	0.01075985967853408	0.0
26	0.0	4.0299099919603294E-5	0.0	0.015434555269208063	0.0
27	0.0	4.0299099919603294E-5	0.0	0.03800205122418591	0.0
28	0.0	4.0299099919603294E-5	0.0	0.1334706189337261	0.0
29	0.0	4.0299099919603294E-5	0.0	0.2616620557779842	0.0
30	0.0	4.0299099919603294E-5	0.0	0.43337652053541387	0.0
31	0.0	4.0299099919603294E-5	0.0	0.844064647816091	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGTA	635	0.0	23.889765	8
TCTAGAC	835	0.0	22.598803	3
CTAGACA	860	0.0	21.94186	4
ACTGATC	465	0.0	21.086021	8
GTCTAGA	645	0.0	20.937984	1
GTTCTAG	965	0.0	20.896374	1
GGTATCA	1740	0.0	20.626438	1
GACATGT	750	0.0	20.226665	7
TAATCCG	55	5.145404E-4	20.181818	9
TAGAACT	705	0.0	19.41844	4
GTATTTT	785	0.0	18.853504	12
TTAACGG	50	0.0070371227	18.5	35
GTATATC	50	0.0070371227	18.5	1
CTAGAAC	745	0.0	18.127516	3
TCTAGTT	1035	0.0	18.05314	28
TATTTTC	820	0.0	18.048782	13
ATACACA	485	0.0	17.927835	37
AGTTTTT	1055	0.0	17.886255	31
CTCTAGT	1045	0.0	17.880383	27
TCGTTTA	705	0.0	17.843971	30
>>END_MODULE