FastQCFastQC Report
Fri 10 Feb 2017
ERR1631315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129310
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76680.3601166575087704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65830.3091611836698273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58260.27360976090846334No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33450.15709314284909195No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC28230.13257815912196907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.12450042502031174No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC25100.11787856159976705No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24650.11576520093363578No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC24410.11463807524503243No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC22000.10331985478864046No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT21820.10247451052218795No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC21740.10209880195932017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG502.7031507E-422.25
GGTATCA34300.018.6078721
GCAGTCG10650.017.718319
GTCTAGA1900.017.5263161
AGTCGGT10600.017.45283111
CTTATAC25100.016.6573737
GTCCTAA2250.016.4444451
GGCAGTC11700.016.2863248
TACTATA2500.016.2799992
CTCTATG17300.016.1473981
TATTAGA1504.6766218E-916.0333332
CGCCTTA8800.015.97727325
CAGTCGG11950.015.94560710
TCGGTGA11450.015.83406213
GGACCGT1302.591787E-715.6538466
TCGTTTA6050.015.59504130
GTCGGTG11900.015.54621812
CGGTGAT12100.015.28925614
TCTATGG18700.015.1363632
TAACCGT750.004105934414.87
Produced by FastQC (version 0.11.5)