##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2129310
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.085716969346876	34.0	33.0	34.0	31.0	34.0
2	33.22909346220137	34.0	33.0	34.0	31.0	34.0
3	33.313252649919455	34.0	34.0	34.0	31.0	34.0
4	36.52539461139994	37.0	37.0	37.0	35.0	37.0
5	36.5172670959137	37.0	37.0	37.0	35.0	37.0
6	36.56810797864097	37.0	37.0	37.0	35.0	37.0
7	36.574109453297076	37.0	37.0	37.0	35.0	37.0
8	36.57803278996482	37.0	37.0	37.0	35.0	37.0
9	38.42454691895497	39.0	39.0	39.0	37.0	39.0
10	38.39547599926737	39.0	39.0	39.0	37.0	39.0
11	38.444720590238155	39.0	39.0	39.0	37.0	39.0
12	38.40722111857832	39.0	39.0	39.0	37.0	39.0
13	38.43318539808671	39.0	39.0	39.0	37.0	39.0
14	39.99306301102235	41.0	40.0	41.0	38.0	41.0
15	39.98310297702073	41.0	40.0	41.0	38.0	41.0
16	39.95228219470157	41.0	40.0	41.0	38.0	41.0
17	39.94716504407531	41.0	40.0	41.0	38.0	41.0
18	39.93018207776228	41.0	40.0	41.0	38.0	41.0
19	39.94571950537968	41.0	40.0	41.0	38.0	41.0
20	39.92101478882831	41.0	40.0	41.0	38.0	41.0
21	39.894633003179436	41.0	40.0	41.0	38.0	41.0
22	39.83512734172103	41.0	40.0	41.0	38.0	41.0
23	39.7745974047931	41.0	40.0	41.0	38.0	41.0
24	39.751557077175235	41.0	40.0	41.0	38.0	41.0
25	39.74522263080529	41.0	40.0	41.0	37.0	41.0
26	39.65159511766723	41.0	40.0	41.0	37.0	41.0
27	39.59987836435277	41.0	40.0	41.0	37.0	41.0
28	39.51956079669001	41.0	40.0	41.0	37.0	41.0
29	39.46578422117963	41.0	39.0	41.0	36.0	41.0
30	39.397536760734695	41.0	39.0	41.0	36.0	41.0
31	39.34009139110792	41.0	39.0	41.0	35.0	41.0
32	39.25999549149724	41.0	39.0	41.0	35.0	41.0
33	39.172648886258926	41.0	39.0	41.0	35.0	41.0
34	39.114475111655885	41.0	39.0	41.0	35.0	41.0
35	39.01456058535394	41.0	39.0	41.0	35.0	41.0
36	38.940992152387395	41.0	39.0	41.0	35.0	41.0
37	38.871668286909845	41.0	39.0	41.0	35.0	41.0
38	38.78622981153519	41.0	38.0	41.0	35.0	41.0
39	38.69023533445107	40.0	38.0	41.0	35.0	41.0
40	38.57899507352147	40.0	38.0	41.0	35.0	41.0
41	38.476123251194046	40.0	38.0	41.0	35.0	41.0
42	38.327434239260604	40.0	37.0	41.0	35.0	41.0
43	37.547541691909586	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	3.0
13	5.0
14	5.0
15	5.0
16	10.0
17	16.0
18	35.0
19	51.0
20	131.0
21	202.0
22	395.0
23	750.0
24	1151.0
25	1814.0
26	2650.0
27	3929.0
28	5740.0
29	8021.0
30	10723.0
31	13607.0
32	17457.0
33	23225.0
34	34943.0
35	52600.0
36	86632.0
37	177672.0
38	388737.0
39	1298796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.715100196777364	17.538968022504942	12.033381705810802	26.712550074906893
2	19.71544772719801	19.74874489858217	32.98946607116859	27.546341303051225
3	20.41276281988062	21.413086868516093	28.33899244356153	29.83515786804176
4	14.588012079030296	15.20976278700612	33.13533492070201	37.06689021326157
5	16.39526419356505	34.80371575768677	31.912309621426658	16.888710427321527
6	35.17740488702913	33.2821430416426	15.859456819345233	15.680995251983038
7	30.97881473341129	29.111214430965898	20.02921134076297	19.880759494859838
8	29.34213430641851	30.659791199966186	19.187999868502004	20.8100746251133
9	27.815536488345987	13.737501819838352	19.045183651041885	39.40177804077377
10	19.0749115910788	24.579887381358283	29.952191085375073	26.393009942187845
11	37.944498452550356	20.91682282053811	19.825483372547914	21.31319535436362
12	23.775918020391583	24.651553789725313	26.76561890941197	24.80690928047114
13	31.280790490816273	17.814456326227745	24.244802306850577	26.659950876105405
14	24.120301881830265	19.600011271256886	23.328918757719634	32.95076808919321
15	27.178522620003665	25.939341852524993	20.81716612423743	26.064969403233913
16	26.817372763947006	24.41809788147334	22.77921016667371	25.985319187905944
17	25.345581432482824	24.938360313904504	23.656771442392134	26.059286811220538
18	25.984285989358053	22.632073300740615	25.335813009848263	26.047827700053066
19	25.775204174122134	24.18783549600575	24.63981289713569	25.39714743273643
20	26.191254443927846	22.909158365855607	24.680013713362545	26.219573476854002
21	27.85498588744711	23.313702560923492	24.206198252015913	24.625113299613492
22	27.40188136062856	22.951660397030025	23.887456499992958	25.75900174234846
23	25.79591510865022	22.978758377126862	24.514232309997137	26.711094204225784
24	25.40719763679314	24.025717251128302	24.536352151635977	26.030732960442588
25	25.84884305244422	23.12744504088179	24.35385171722295	26.669860189451043
26	25.91402848810178	24.147399861927102	24.482907608568034	25.455664041403082
27	26.52915733265706	23.017127614109736	24.24682174037599	26.206893312857215
28	25.15279597616129	23.467226472425338	25.22389882168402	26.156078729729348
29	24.66996350930583	23.721440278775756	25.5400106137669	26.06858559815151
30	24.316468715217606	24.55720397687514	25.698606590867463	25.427720717039794
31	25.753319150335084	24.024073526165754	24.081134264151295	26.141473059347863
32	24.964659913305248	23.72237955018292	24.600974024449236	26.71198651206259
33	24.438761852431067	23.154167312415762	25.597306169604238	26.809764665548936
34	25.906185571851914	23.106968924205493	25.00157327960701	25.98527222433558
35	25.62557823896004	23.068599687222623	25.82991673358975	25.475905340227584
36	24.10165734439795	23.96884436742419	25.474355542405757	26.455142745772104
37	25.12551014178302	22.765825549121548	26.065720820359644	26.042943488735787
38	24.078081632077996	22.0979566150537	27.083750135020264	26.740211617848036
39	24.633660669418735	21.87929423146465	26.96051772639963	26.526527372716984
40	23.862237062710456	22.267260286196	27.61251297368631	26.257989677407235
41	22.851674955736833	22.481461130600994	28.51707830236086	26.14978561130131
42	23.696079950782178	22.599809327904342	28.281978669146348	25.422132052167136
43	22.29031939924201	21.920246464817243	28.563431346304675	27.226002789636077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	232.0
2	271.0
3	786.5
4	1302.0
5	1302.0
6	1659.5
7	2017.0
8	1932.0
9	1847.0
10	2754.5
11	3662.0
12	3662.0
13	6188.0
14	8714.0
15	12426.0
16	16138.0
17	14563.0
18	12988.0
19	12988.0
20	14472.5
21	15957.0
22	12894.5
23	9832.0
24	10857.5
25	11883.0
26	11883.0
27	14127.5
28	16372.0
29	19922.0
30	23472.0
31	27681.5
32	31891.0
33	31891.0
34	38905.0
35	45919.0
36	56606.0
37	67293.0
38	77051.0
39	86809.0
40	86809.0
41	94747.0
42	102685.0
43	110897.0
44	119109.0
45	128401.0
46	137693.0
47	137693.0
48	149541.5
49	161390.0
50	163995.0
51	166600.0
52	168743.0
53	170886.0
54	170886.0
55	165277.5
56	159669.0
57	153879.0
58	148089.0
59	144397.5
60	140706.0
61	140706.0
62	132909.0
63	125112.0
64	114373.0
65	103634.0
66	91931.0
67	80228.0
68	80228.0
69	68040.5
70	55853.0
71	48114.0
72	40375.0
73	30683.0
74	20991.0
75	20991.0
76	16811.5
77	12632.0
78	10465.0
79	8298.0
80	7441.5
81	6585.0
82	6585.0
83	5470.5
84	4356.0
85	3981.5
86	3607.0
87	3174.0
88	2741.0
89	2741.0
90	1990.5
91	1240.0
92	716.0
93	192.0
94	115.0
95	38.0
96	38.0
97	37.5
98	37.0
99	20.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2129310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.539228694452234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.55554815705206	42.383558662314236
2	7.4514603697369575	7.382791987280934
3	2.407126430130263	3.577415601560505
4	1.1676592326970745	2.3137975106307596
5	0.6955594502021928	1.7228739337076941
6	0.4836533167660275	1.437588736086155
7	0.34205231220045657	1.1861505403695214
8	0.24431419429731238	0.9682509395676318
9	0.19279332355260315	0.8595749291612338
>10	1.1982711453998527	11.293019620420823
>50	0.1276015798499099	4.429642268904269
>100	0.11089203990405601	11.119079623001763
>500	0.015663761131240093	5.143838862228192
>1k	0.0071198914232909515	5.238225109761878
>5k	2.8479565693163806E-4	0.944191675004392
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7668	0.3601166575087704	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6583	0.3091611836698273	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5826	0.27360976090846334	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3345	0.15709314284909195	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2823	0.13257815912196907	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2651	0.12450042502031174	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2510	0.11787856159976705	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2465	0.11576520093363578	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2441	0.11463807524503243	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2200	0.10331985478864046	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2182	0.10247451052218795	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2174	0.10209880195932017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.696357035847293E-5	0.0	0.0	0.0
4	0.0	4.696357035847293E-5	0.0	0.0	0.0
5	0.0	4.696357035847293E-5	0.0	4.696357035847293E-5	0.0
6	0.0	4.696357035847293E-5	0.0	4.696357035847293E-5	0.0
7	0.0	4.696357035847293E-5	0.0	4.696357035847293E-5	0.0
8	0.0	4.696357035847293E-5	0.0	4.696357035847293E-5	0.0
9	0.0	4.696357035847293E-5	0.0	4.696357035847293E-5	0.0
10	0.0	4.696357035847293E-5	0.0	9.392714071694586E-5	0.0
11	0.0	4.696357035847293E-5	0.0	5.165992739432023E-4	0.0
12	0.0	4.696357035847293E-5	0.0	0.0010331985478864046	0.0
13	0.0	4.696357035847293E-5	0.0	0.0011740892589618232	0.0
14	0.0	4.696357035847293E-5	0.0	0.0016437249625465527	0.0
15	0.0	4.696357035847293E-5	0.0	0.0023951420882821197	0.0
16	0.0	4.696357035847293E-5	0.0	0.00432064847297951	0.0
17	0.0	4.696357035847293E-5	0.0	0.0061991912873184275	0.0
18	0.0	4.696357035847293E-5	0.0	0.00704453555377094	0.0
19	0.0	4.696357035847293E-5	0.0	0.007655061968431088	0.0
20	0.0	4.696357035847293E-5	0.0	0.0085004062348836	0.0
21	0.0	4.696357035847293E-5	0.0	0.0102380583381471	0.0
22	0.0	4.696357035847293E-5	0.0	0.013243726841089367	0.0
23	0.0	4.696357035847293E-5	0.0	0.017188666751201093	0.0
24	0.0	4.696357035847293E-5	0.0	0.023153040186727154	0.0
25	0.0	4.696357035847293E-5	0.0	0.026628344393254153	0.0
26	0.0	4.696357035847293E-5	0.0	0.03550445919100554	0.0
27	0.0	4.696357035847293E-5	0.0	0.07077410053021871	0.0
28	0.0	4.696357035847293E-5	0.0	0.1835805965312707	0.0
29	0.0	4.696357035847293E-5	0.0	0.34151908364681516	0.0
30	0.0	4.696357035847293E-5	0.0	0.5642203342866938	0.0
31	0.0	4.696357035847293E-5	0.0	1.073164546261465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	50	2.7031507E-4	22.2	5
GGTATCA	3430	0.0	18.607872	1
GCAGTCG	1065	0.0	17.71831	9
GTCTAGA	190	0.0	17.526316	1
AGTCGGT	1060	0.0	17.452831	11
CTTATAC	2510	0.0	16.65737	37
GTCCTAA	225	0.0	16.444445	1
GGCAGTC	1170	0.0	16.286324	8
TACTATA	250	0.0	16.279999	2
CTCTATG	1730	0.0	16.147398	1
TATTAGA	150	4.6766218E-9	16.033333	2
CGCCTTA	880	0.0	15.977273	25
CAGTCGG	1195	0.0	15.945607	10
TCGGTGA	1145	0.0	15.834062	13
GGACCGT	130	2.591787E-7	15.653846	6
TCGTTTA	605	0.0	15.595041	30
GTCGGTG	1190	0.0	15.546218	12
CGGTGAT	1210	0.0	15.289256	14
TCTATGG	1870	0.0	15.136363	2
TAACCGT	75	0.0041059344	14.8	7
>>END_MODULE