FastQCFastQC Report
Fri 10 Feb 2017
ERR1631314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344441
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA27120.2017195250665518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.17546325945132585No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22630.16832274528967803No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC22150.1647524882088541No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC22020.1637855435827976No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20930.1556780847950933No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT20160.1499507973946049No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC20000.14876071170099692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18860.14028135113404008No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC18700.1390912654404321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17460.1298681013149703No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG17420.12957057989156834No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC17310.12875239597721283No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG17190.12785983170700685No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC16610.12354577106767795No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG16440.12228130501821946No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT15710.11685153904113309No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA15490.1152151712124221No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA15100.11231433733425267No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG14710.10941350345608322No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14200.10562010530770782No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA14190.10554572495185732No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG14160.10532258388430582No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC13900.10338869463219286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC358.870183E-426.428573
TGTACGA451.3233755E-424.6666662
TCTAGAC1700.021.7647083
GGTATCA14900.021.7281881
CATATAC603.7268917E-521.5833343
GTTCTAG1750.021.1428571
GCCTAAT450.003826141120.5555551
ACGTAGC450.003826141120.5555551
TCGCGAA951.6766353E-719.47368411
CTAGTTT1457.2759576E-1219.1379329
CTAGACA1900.018.54
CTATACG609.2379266E-418.527
TAAGGTA609.2379266E-418.55
AGACCGA500.007035489718.4999986
CTCTAGT1653.6379788E-1217.93939627
CGCCTTA2700.017.81481625
ATCGCGA852.723671E-517.41176610
CTTATAC18850.017.37135337
GCCGTTA752.0675948E-417.26666615
AAGACGG6300.017.0317465
Produced by FastQC (version 0.11.5)