##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1344441
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06580281321382	34.0	33.0	34.0	31.0	34.0
2	33.208361690844	34.0	33.0	34.0	31.0	34.0
3	33.29430893583281	34.0	34.0	34.0	31.0	34.0
4	36.51818190608588	37.0	37.0	37.0	35.0	37.0
5	36.510210563349375	37.0	37.0	37.0	35.0	37.0
6	36.5570664685174	37.0	37.0	37.0	35.0	37.0
7	36.55832721554906	37.0	37.0	37.0	35.0	37.0
8	36.556494483580906	37.0	37.0	37.0	35.0	37.0
9	38.407962119572375	39.0	39.0	39.0	37.0	39.0
10	38.36851970447197	39.0	39.0	39.0	37.0	39.0
11	38.42315281964772	39.0	39.0	39.0	37.0	39.0
12	38.37812964644785	39.0	39.0	39.0	37.0	39.0
13	38.408611460078944	39.0	39.0	39.0	37.0	39.0
14	39.950603261876125	41.0	40.0	41.0	38.0	41.0
15	39.95016962440152	41.0	40.0	41.0	38.0	41.0
16	39.9177576405361	41.0	40.0	41.0	38.0	41.0
17	39.90225008014483	41.0	40.0	41.0	38.0	41.0
18	39.87438124841477	41.0	40.0	41.0	38.0	41.0
19	39.88470970462817	41.0	40.0	41.0	38.0	41.0
20	39.858141785321926	41.0	40.0	41.0	38.0	41.0
21	39.81864879157955	41.0	40.0	41.0	38.0	41.0
22	39.757223262307534	41.0	40.0	41.0	38.0	41.0
23	39.676983965826686	41.0	40.0	41.0	37.0	41.0
24	39.644975123489985	41.0	40.0	41.0	37.0	41.0
25	39.64311115177237	41.0	40.0	41.0	37.0	41.0
26	39.55776638766595	41.0	40.0	41.0	37.0	41.0
27	39.49409977827216	41.0	39.0	41.0	37.0	41.0
28	39.389267360932905	41.0	39.0	41.0	36.0	41.0
29	39.33458292331162	41.0	39.0	41.0	36.0	41.0
30	39.268725812438035	41.0	39.0	41.0	35.0	41.0
31	39.23330886219626	41.0	39.0	41.0	35.0	41.0
32	39.15030633549557	41.0	39.0	41.0	35.0	41.0
33	39.047510452299505	41.0	39.0	41.0	35.0	41.0
34	38.99200113653184	41.0	39.0	41.0	35.0	41.0
35	38.87660447725114	41.0	39.0	41.0	35.0	41.0
36	38.82188954368395	40.0	38.0	41.0	35.0	41.0
37	38.75467052849474	40.0	38.0	41.0	35.0	41.0
38	38.67540858988978	40.0	38.0	41.0	35.0	41.0
39	38.596790041362915	40.0	38.0	41.0	35.0	41.0
40	38.48637389070997	40.0	38.0	41.0	35.0	41.0
41	38.391936128100824	40.0	37.0	41.0	35.0	41.0
42	38.24885361276545	40.0	37.0	41.0	35.0	41.0
43	37.42700200306298	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	0.0
15	2.0
16	7.0
17	10.0
18	26.0
19	57.0
20	89.0
21	174.0
22	303.0
23	498.0
24	817.0
25	1228.0
26	1808.0
27	2552.0
28	3518.0
29	4814.0
30	6413.0
31	8629.0
32	11032.0
33	15104.0
34	23702.0
35	36277.0
36	62492.0
37	120864.0
38	266953.0
39	777069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71125248337413	17.29320959417334	11.609880983992603	27.385656938459924
2	20.441283775189838	19.32282636426589	31.773726031860082	28.46216382868419
3	20.51335834000897	20.469697071124727	28.26527902674792	30.75166556211838
4	14.796409808983807	14.776773395039278	32.709654049526904	37.717162746450015
5	17.35732546091647	33.64163990833365	31.462593003337446	17.538441627412436
6	36.215348981472594	33.03261355462977	15.21926213199389	15.532775331903743
7	31.637386839586117	28.480907678358513	19.621389112649794	20.260316369405572
8	29.642505695675748	29.556298863245022	19.03757769957923	21.7636177415
9	27.87136066216368	13.411521963403377	18.593526975151757	40.12359039928119
10	19.567463354658184	23.468192356525872	29.047090947092506	27.917253341723438
11	39.89769725856323	19.807191241564336	19.232454231907536	21.0626572679649
12	24.760922941207536	23.6962425275635	25.52406539223365	26.018769138995314
13	31.790833513705696	16.398785815071097	23.654887049710624	28.155493621512584
14	24.65403836985037	19.264586545634952	21.73252675275449	34.34884833176019
15	27.976460104980433	25.278833358994557	19.4810333811599	27.26367315486511
16	28.140394409274933	23.608324946948212	21.49785673004617	26.75342391373069
17	26.873771329496794	23.741986446411556	22.25155287587927	27.13268934821238
18	27.467549710251323	21.231054393610428	23.989301129614464	27.31209476652378
19	27.033689094575365	23.17677012230362	22.817810524969115	26.9717302581519
20	27.440698401789295	21.61448512801975	22.696496164576953	28.248320305614005
21	29.802795362533573	22.43237152095183	22.339172935071154	25.425660181443437
22	29.16111603261132	21.815088947748542	21.93543636351465	27.088358656125482
23	26.988540218574116	21.578038753653004	23.156389904800584	28.277031122972296
24	26.436712358519266	23.076654163328847	22.988067159510905	27.498566318640982
25	27.069019763604352	21.737807758019876	22.810446869739913	28.382725608635855
26	27.06284619406876	23.418803800241143	22.913538042948705	26.604811962741394
27	27.990443611880327	21.57417097514878	22.722157387345373	27.71322802562552
28	26.336224497765244	22.343710136778036	23.915962098745872	27.404103266710848
29	25.820024828162786	22.723868135529933	24.10354935620083	27.352557680106454
30	25.019840959923123	23.757829462207713	24.613129174132595	26.609200403736573
31	27.224325946620194	23.015290369752186	22.174420446862303	27.585963236765316
32	25.947215236667137	22.316635687248453	22.954149717243077	28.781999358841333
33	25.196866206847307	21.723824251119982	24.341045832431472	28.73826370960124
34	26.99649891665012	21.83271709208511	24.089119567165834	27.08166442409894
35	26.963920320787597	21.60094790325496	25.122560231352658	26.312571544604786
36	24.89317121390972	22.81282704112713	24.642509414693542	27.651492330269605
37	25.869636525515066	21.248161875456045	25.512610817432673	27.369590781596216
38	24.690559124572964	20.48345743695707	26.80147362361011	28.024509814859854
39	25.271767225188757	19.955877572909483	26.773060327675218	27.99929487422654
40	24.436922111122765	20.958896671553457	27.516120082621697	27.08806113470208
41	23.23471241951116	20.796301213664268	28.991008158781234	26.97797820804334
42	24.351979744741495	21.12208717228945	28.643875038026955	25.882058044942095
43	22.293429016223097	19.982282599236413	28.983570123196184	28.740718261344306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	35.5
2	44.0
3	150.0
4	256.0
5	256.0
6	332.0
7	408.0
8	416.5
9	425.0
10	629.5
11	834.0
12	834.0
13	1517.5
14	2201.0
15	3400.0
16	4599.0
17	4270.5
18	3942.0
19	3942.0
20	4416.5
21	4891.0
22	4196.0
23	3501.0
24	4029.0
25	4557.0
26	4557.0
27	5545.0
28	6533.0
29	8315.5
30	10098.0
31	12489.5
32	14881.0
33	14881.0
34	19515.0
35	24149.0
36	30730.5
37	37312.0
38	44403.5
39	51495.0
40	51495.0
41	56047.0
42	60599.0
43	64730.0
44	68861.0
45	74659.0
46	80457.0
47	80457.0
48	88359.5
49	96262.0
50	99338.0
51	102414.0
52	106213.5
53	110013.0
54	110013.0
55	107463.5
56	104914.0
57	103328.0
58	101742.0
59	100757.0
60	99772.0
61	99772.0
62	93490.0
63	87208.0
64	81967.5
65	76727.0
66	68611.5
67	60496.0
68	60496.0
69	51989.0
70	43482.0
71	37710.0
72	31938.0
73	24033.0
74	16128.0
75	16128.0
76	12851.5
77	9575.0
78	8115.5
79	6656.0
80	6245.5
81	5835.0
82	5835.0
83	4810.5
84	3786.0
85	3631.0
86	3476.0
87	3006.0
88	2536.0
89	2536.0
90	1857.5
91	1179.0
92	662.5
93	146.0
94	104.5
95	63.0
96	63.0
97	40.0
98	17.0
99	11.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1344441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.932746496255604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.14917181290637	40.03000816240853
2	6.701217499946546	6.156106492826329
3	1.9696516353406073	2.714145277561062
4	0.9753718318279224	1.7920602836376167
5	0.6018672800163103	1.3822708598690032
6	0.3926872079623688	1.0822321185394734
7	0.29829944778968764	0.9591199040707729
8	0.21036908500168863	0.773026388162545
9	0.1706923483881639	0.705633153062767
>10	1.168521102796544	10.667870281580953
>50	0.172486889780074	5.600710197740124
>100	0.1594605401302844	15.554707243494317
>500	0.020622695701162062	6.366909969989733
>1k	0.009580622412350878	6.215199667056822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2712	0.2017195250665518	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2359	0.17546325945132585	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2263	0.16832274528967803	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2215	0.1647524882088541	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2202	0.1637855435827976	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2093	0.1556780847950933	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2016	0.1499507973946049	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2000	0.14876071170099692	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1886	0.14028135113404008	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1870	0.1390912654404321	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1746	0.1298681013149703	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	1742	0.12957057989156834	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1731	0.12875239597721283	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	1719	0.12785983170700685	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1661	0.12354577106767795	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1644	0.12228130501821946	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1571	0.11685153904113309	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	1549	0.1152151712124221	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1510	0.11231433733425267	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1471	0.10941350345608322	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1420	0.10562010530770782	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	1419	0.10554572495185732	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	1416	0.10532258388430582	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1390	0.10338869463219286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	7.438035585049846E-5	0.0	0.0	0.0
11	0.0	7.438035585049846E-5	0.0	7.438035585049846E-5	0.0
12	0.0	7.438035585049846E-5	0.0	7.438035585049846E-5	0.0
13	0.0	7.438035585049846E-5	0.0	1.4876071170099692E-4	0.0
14	0.0	7.438035585049846E-5	0.0	1.4876071170099692E-4	7.438035585049846E-5
15	0.0	7.438035585049846E-5	0.0	3.719017792524923E-4	7.438035585049846E-5
16	0.0	7.438035585049846E-5	0.0	9.6694462605648E-4	7.438035585049846E-5
17	0.0	7.438035585049846E-5	0.0	0.0017107481845614646	7.438035585049846E-5
18	0.0	7.438035585049846E-5	0.0	0.001785128540411963	7.438035585049846E-5
19	0.0	7.438035585049846E-5	0.0	0.00193388925211296	7.438035585049846E-5
20	0.0	7.438035585049846E-5	0.0	0.0023801713872159506	7.438035585049846E-5
21	0.0	7.438035585049846E-5	0.0	0.0029008338781694397	7.438035585049846E-5
22	0.0	7.438035585049846E-5	0.0	0.00386777850422592	7.438035585049846E-5
23	0.0	7.438035585049846E-5	0.0	0.005950428468039877	7.438035585049846E-5
24	0.0	7.438035585049846E-5	0.0	0.009000023057910313	7.438035585049846E-5
25	0.0	7.438035585049846E-5	0.0	0.011677715868528258	7.438035585049846E-5
26	0.0	7.438035585049846E-5	0.0	0.018966990741877108	7.438035585049846E-5
27	0.0	7.438035585049846E-5	0.0	0.05942990432454827	7.438035585049846E-5
28	0.0	7.438035585049846E-5	0.0	0.20915756065160168	7.438035585049846E-5
29	0.0	7.438035585049846E-5	0.0	0.40916633753359205	7.438035585049846E-5
30	0.0	7.438035585049846E-5	0.0	0.679390170338453	7.438035585049846E-5
31	0.0	7.438035585049846E-5	0.0	1.3645820084332447	7.438035585049846E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAC	35	8.870183E-4	26.42857	3
TGTACGA	45	1.3233755E-4	24.666666	2
TCTAGAC	170	0.0	21.764708	3
GGTATCA	1490	0.0	21.728188	1
CATATAC	60	3.7268917E-5	21.583334	3
GTTCTAG	175	0.0	21.142857	1
GCCTAAT	45	0.0038261411	20.555555	1
ACGTAGC	45	0.0038261411	20.555555	1
TCGCGAA	95	1.6766353E-7	19.473684	11
CTAGTTT	145	7.2759576E-12	19.13793	29
CTAGACA	190	0.0	18.5	4
CTATACG	60	9.2379266E-4	18.5	27
TAAGGTA	60	9.2379266E-4	18.5	5
AGACCGA	50	0.0070354897	18.499998	6
CTCTAGT	165	3.6379788E-12	17.939396	27
CGCCTTA	270	0.0	17.814816	25
ATCGCGA	85	2.723671E-5	17.411766	10
CTTATAC	1885	0.0	17.371353	37
GCCGTTA	75	2.0675948E-4	17.266666	15
AAGACGG	630	0.0	17.031746	5
>>END_MODULE